Genetic diversity of subgenotype 2.1 isolates of classical swine fever virus

Gong, Wenjie; Wu, Jianmin; Lu, Zexi; Zhang, Li; Qin, Shaomin; Chen, Fenglian; Peng, Zhicheng; Wang, Qin; Ma, Ling; Bai, Anbin; Guo, Huancheng; Shi, Jishu; Tu, Changchun

doi:10.1016/j.meegid.2016.04.002

Cited by 46 publications

(37 citation statements)

References 25 publications

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“…Nevertheless, development of new subunit vaccines remains a major area of research with the goal of fulfilling the following: early onset of protection with sterile immunity, long‐lasting immunity (the animal life cycle in the pork industry is about 7–8 months), safety without viral shedding among animals and low‐cost production. Importantly, subunit vaccines provide alternatives to protect pig populations against emerging subgenotypes for which current live attenuated CSFV vaccines are not highly protective, such as the emerging subgenotype 2.1d recently reported in India (Gong et al ., ) and south‐east Asia (Luo et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Plant‐made E2 glycoprotein single‐dose vaccine protects pigs against classical swine fever

Laughlin

Madera

Peres³

et al. 2018

Plant Biotechnology Journal

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Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa and South America had significant adverse impacts on animal health, food security and the pig industry. The disease is generally contained by prevention of exposure through import restrictions (e.g. banning import of live pigs and pork products), localized vaccination programmes and culling of infected or at-risk animals, often at very high cost. Current CSFV-modified live virus vaccines are protective, but do not allow differentiation of infected from vaccinated animals (DIVA), a critical aspect of disease surveillance programmes. Alternatively, first-generation subunit vaccines using the viral protein E2 allow for use of DIVA diagnostic tests, but are slow to induce a protective response, provide limited prevention of vertical transmission and may fail to block viral shedding. CSFV E2 subunit vaccines from a baculovirus/insect cell system have been developed for several vaccination campaigns in Europe and Asia. However, this expression system is considered expensive for a veterinary vaccine and is not ideal for wide-spread deployment. To address the issues of scalability, cost of production and immunogenicity, we have employed an Agrobacterium-mediated transient expression platform in Nicotiana benthamiana and formulated the purified antigen in novel oil-in-water emulsion adjuvants. We report the manufacturing of adjuvanted, plant-made CSFV E2 subunit vaccine. The vaccine provided complete protection in challenged pigs, even after single-dose vaccination, which was accompanied by strong virus neutralization antibody responses.

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Section: Discussionmentioning

confidence: 99%

Plant‐made E2 glycoprotein single‐dose vaccine protects pigs against classical swine fever

Laughlin

Madera

Peres³

et al. 2018

Plant Biotechnology Journal

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“…In mainland China, CSFV isolates circulating in the late 1980s and 1990s consisted of four subgenotypes (1.1, 2.1, 2.2, and 2.3) (5). More recently, the genetic diversity of subgenotype 2.1 isolates was analyzed, and 10 sub-subgenotypes (2.1a to 2.1j) have been identified (6, 7). A greater understanding of the mechanisms of emergence of subgenotype 2.1 isolates will require analysis of additional complete genomes.…”

Section: Genome Announcementmentioning

confidence: 99%

“…GD317/2011 was isolated from a pig kidney collected from Guangdong, China, in 2011 and classified as sub-subgenotype 2.1i by phylogenetic analysis of the complete E2 gene sequences (6). To obtain the entire genome sequence of this 2.1i isolate, total RNA was extracted from a 10% homogenate of the kidney sample using the TRIzol reagent (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Sub-Subgenotype 2.1i Isolate of Classical Swine Fever Virus from China

Zhang

Bao

et al. 2017

Genome Announc

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“…-6]. It has been reported that subgenotype 2.1 isolates are distributed in many Asian and European countries and play a dominant role in CSF outbreaks occurring in China, Korea and other countries [3,5,7,8]. With more sequences of 2.1 isolates deposited in the GenBank database, CSFV subgenotype 2.1 has been further divided into 10 sub-subgenotypes (2.1a-2.1j) based on a nucleotide sequence of 190-nt and the 1,119-nt E2 gene [8].…”

mentioning

confidence: 99%

“…It has been reported that subgenotype 2.1 isolates are distributed in many Asian and European countries and play a dominant role in CSF outbreaks occurring in China, Korea and other countries [3,5,7,8]. With more sequences of 2.1 isolates deposited in the GenBank database, CSFV subgenotype 2.1 has been further divided into 10 sub-subgenotypes (2.1a-2.1j) based on a nucleotide sequence of 190-nt and the 1,119-nt E2 gene [8]. Except for viruses in the silent sub-subgenotypes (2.1a, 2.1e, and 2.1f), CSFV isolates of subgenotype 2.1 are active and still circulate to cause CSFV infection in domestic pigs and wild boar in many countries, and W. Gong Kidney sample GD19/2011 from a CSF-suspected pig on a pig farm in Guangdong province was collected on July 9, 2011, and submitted to our laboratory for diagnosis.…”

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confidence: 99%

Complete genome sequence of a novel sub-subgenotype 2.1g isolate of classical swine fever virus from China

Gong

Zhang

et al. 2016

Arch Virol

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Current subgenotype 2.1 isolates of classical swine fever virus (CSFV) play a dominant role in CSF outbreaks in China, and a novel sub-subgenotype 2.1g of CSFV was recently identified, but the complete genome sequence of this new sub-subgenotype has not been reported. In this study, complete genome of 2.1g isolate GD19/2011 collected from Guangdong province of China in 2011 was sequenced. It was found to be 12,298 nucleotides (nt) in length, including a 375-nt 5'UTR, a 11,697-nt opening reading frame (ORF), and a 227-nt 3'UTR. GD19/2011 shared 91.0-93.7 % and 95.6-97.5 % nt and amino acid sequence identity, respectively, with other subgenotype 2.1 isolates. The topology of a phylogenetic tree constructed based on complete genome sequences of GD19/2011 and other CSFV isolates was identical to that obtained with full-length E2 gene sequences, but it was significantly different from those obtained with the 5'UTR and core sequences. Serial passages of GD9/2011 in PK-15 cells generated a highly cell-adapted virus stock with an infectious titer of 10(7.8) TCID50/ml at the 12(th) passage in which two amino acid substitutions, S476R and N2494S, were observed in comparison with the complete polyprotein sequence of the original isolate from kidney tissue, GD19/2011. This is the first report of the complete genome sequence of a 2.1g isolate, and the GD19/2011 isolate will be useful for further analysis of the evolution and virulence of CSFV isolates.

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Genetic diversity of subgenotype 2.1 isolates of classical swine fever virus

Cited by 46 publications

References 25 publications

Plant‐made E2 glycoprotein single‐dose vaccine protects pigs against classical swine fever

Plant‐made E2 glycoprotein single‐dose vaccine protects pigs against classical swine fever

Complete Genome Sequence of a Sub-Subgenotype 2.1i Isolate of Classical Swine Fever Virus from China

Complete genome sequence of a novel sub-subgenotype 2.1g isolate of classical swine fever virus from China

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