Genetic diversity of rhizobia nodulating common bean (Phaseolus vulgaris L.) in the Central Black Sea Region of Turkey

Gürkanlı, Cem Tolga; Özkoç, İbrahim; Gündüz, İslam

doi:10.1007/s13213-012-0551-3

Cited by 10 publications

(6 citation statements)

References 88 publications

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“…I have also been isolated from nodules of P. vulgaris , including Rhizobium sp. 1648, 1652, and 1706 from China 65 , CTG-412 and CTG-419 from Turkey 71 , L1, B1 and G2 from Iran 5 , GR12 from Spain 44 , 72 , Rhizobium sp. 9 T and 13 T from Croatia 73 .…”

Section: Resultsmentioning

confidence: 99%

“…According to the recA - atpD phylogeny (Supplementary Fig. S7 ), PVTN21 was phylogenetically related to several strains isolated from P. vulgaris nodules, such as the strains JJW1, L101, 1587, 1617 and 1532 from China 6 , 65 , 70 , NAK368 and NAK378 from Kenya 74 , RHM67 and RHM19 from Morocco 63 , Kim5 from USA 75 , IE4803, IE950, IE4874, and IE4794 from Mexico 43 and CTG-423 and CTG-430 from Turkey 71 . All strains were grouped in a well-supported cluster (Clade 2) containing R. sophoriradicis CCBAU 03470 T as well as the strains JJW1, L101, Kim5, and IE4803, which were recently assigned to R. sophoriradicis based on genomic data 6 .…”

Section: Resultsmentioning

confidence: 99%

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Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Efstathiadou

Ntatsi

Savvas

et al. 2021

Sci Rep

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Phaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the common bean nodulating rhizobia are mainly affiliated to the genus Rhizobium, though strains belonging to Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia have also been reported. This is the first report on the characterization of bean-nodulating rhizobia at the species and symbiovar level in Greece. The goals of this research were to isolate and characterize rhizobia nodulating local common bean genotypes grown in five different edaphoclimatic regions of Greece with no rhizobial inoculation history. The genetic diversity of the rhizobial isolates was assessed by BOX-PCR and the phylogenetic affiliation was assessed by multilocus sequence analysis (MLSA) of housekeeping and symbiosis-related genes. A total of fifty fast-growing rhizobial strains were isolated and representative isolates with distinct BOX-PCR fingerpriniting patterns were subjected to phylogenetic analysis. The strains were closely related to R. anhuiense, R. azibense, R. hidalgonense, R. sophoriradicis, and to a putative new genospecies which is provisionally named as Rhizobium sp. I. Most strains belonged to symbiovar phaseoli carrying the α-, γ-a and γ-b alleles of nodC gene, while some of them belonged to symbiovar gallicum. To the best of our knowledge, it is the first time that strains assigned to R. sophoriradicis and harbored the γ-b allele were found in European soils. All strains were able to re-nodulate their original host, indicating that they are true microsymbionts of common bean.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Efstathiadou

Ntatsi

Savvas

et al. 2021

Sci Rep

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“…Aligned sequences from the selected genes were concatenated and 3120 bp positions were obtained (Table S5). The housekeeping genes gyrB , recA and glnII have been used widely in phylogenetic studies of Ensifer strains, while, recently, the core genes truA , thrA and SMc00019 were demonstrated to produce phylogenies congruent with the reference species tree of rhizobia . The discriminatory power of the selected gene sequences used for the Ensifer taxa was estimated by the number of parsimony‐informative sites contained in each gene (Table S5).…”

Section: Resultsmentioning

confidence: 99%

A novel symbiovar (aegeanense) of the genus Ensifer nodulates Vigna unguiculata

et al. 2017

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“…Currently, MLSA is used widely in bacterial species definition [45] and the atpD, gln II , gyrB, recA and ropB genes are those that can differentiate defined rhizobial species [15,37,45]. In the present study, these five genes were amplified from the representative isolates with the previously reported methods and primer sets atpD255F/atpD782R, glnII12F/glnII6810R, gryB343F/gryB1043R, recA41F/recA640R and rpoB454F/rpoB1364R, respectively [37,45].…”

Section: Methodsmentioning

confidence: 99%

Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico

Verástegui-Valdés

Zhang

Rivera-Orduña

et al. 2014

Systematic and Applied Microbiology

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In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of “R. leguminosarum” and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that “R. leguminosarum” and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the “R. leguminosarum” population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.

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Genetic diversity of rhizobia nodulating common bean (Phaseolus vulgaris L.) in the Central Black Sea Region of Turkey

Cited by 10 publications

References 88 publications

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

A novel symbiovar (aegeanense) of the genus Ensifer nodulates Vigna unguiculata

Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico

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