Genetic diversity of Rehmannia glutinosa cultivars based on sequence-related amplified polymorphism markers

Zhou, Yanqing; Gu, Fengping; Zhou, Chune; Yao, Huanling; Duan, Hongying; Wang, Fang; Liu, Yanju; Xing, Yanmei; Chu, Suxia

doi:10.1016/j.scienta.2010.05.022

Cited by 11 publications

(9 citation statements)

References 24 publications

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“…The clustering analysis indicated cultivars and wild strains were easily distinguished. Grouped results of cultivated strains were basically consistent with that grouped based on RAPD, ISSR (Zhou et al, 2007;Wang et al, 2008), SRAP (Zhou et al, 2010;Zhou et al, 2012) markers, but with a handful of difference (Fig. 6).…”

Section: Discussionsupporting

confidence: 81%

“…The tuberous root is its main medicinal tissue, containing various bioactive components including sugars, amino acids, vitamins, iridoid, aucubin, approximately 70 of which have been identified to date (Zhang et al, 2008). More than 50 cultivars of R. glutinosa are common in Henan, Shandong, Jiangsu, and other provinces in China, at total acreage of more than 8,600 to 9,300 hectares with an annual output of approximately 80 thousand ton (Zhou et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…The genetic relationship among R. glutinosa germplasms remains unclear, due to their complex genetic backgrounds, continuous vegetative propagation and the high heterozygosity of each cultivar (Zhou et al, 2010), making it difficult to select varieties of R. glutinosa with high heterosis. Moreover, biological characteristics for most R. glutinosa cultivars lack definition and among them there is no obvious difference in appearance (Wu et al, 2007); thus, a cultivar is easily mixed with another one in the main production regions (Zhou et al, 2007;Zhou et al, 2010), significantly increasing rate of genetic variation. The cultivars of R. glutinosa in production are thus gradually graded with prolonged planting times.…”

Section: Introductionmentioning

confidence: 99%

“…Although a few molecular markers, including AFLP (Yuan and Hong, 2003), RAPD (Chen et al, 2002;Zhang et al, 2006;Wu et al, 2007;Zhou et al, 2007;Wang et al, 2008), ISSR (Zhou et al, 2007, Wang et al, 2008, SRAP (Zhou et al, 2010) have been reported to identify genetic relationships in various R. glutinosa germplasms, their utilization has been restricted by limited polymorphic information content (Zhang et al, 2012). A recently published set of 15 polymorphic EST-SSR markers that were identified based on Rehmannia species are available (Liu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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SSR Development and Utilization with Rehmannia glutinosa Transcriptome

Li¹,

Yang²,

Feng³

et al. 2016

IJAB

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Rehmannia glutinosa Libosch is a well-known medicinal plant in China. Despite its common use, very few molecular markers are available for this species, severely impeding any improvement of key agronomic traits. In this study, gene-based SSR (simple sequence repeat) markers were mined from the R. glutinosa transcriptome, assembled from leaf and root libraries, to obtain polymorphic information content (PIC). A total of 1,812 SSR (≥18 bp) loci were identified from 1,747 transcripts, in which 1,018 transcripts had significant homology to known proteins, and 35 transcripts were identified as non-coding RNA. Of all SSR loci, AG/GT was the most frequent SSRs repeat type, and bias of base composition presented SSRs loci from noncoding RNA. A set of 279 SSR primers (≥20 bp) were synthesized, of which 204 were successfully amplified in the Wen85-5 genome and 91 had a polymorphic information content between 0.33 and 0.90. Seven pairs of polymorphic SSR primers were selected to evaluate the genetic diversity of 36 R. glutinosa germplasms, and results demonstrated that cultivars are differ considerably from wild strains and that distinct genetic relationships exist among wild strains. The SSRs identified in this study provide critical information for future R. glutinosa breeding.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

SSR Development and Utilization with Rehmannia glutinosa Transcriptome

Li¹,

Yang²,

Feng³

et al. 2016

IJAB

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“…However, little is known about the presence of flavonoids in the plants; only one study by Chung et al (2006) reports the production of small amounts of hesperidin, naringin and myricetin and traces of quercetin, naringenin and kaempferol. Annual demand of fresh Rehmannia root is 2 9 10 7 kg, which sells at a price of 1,200 USD/kg (Zhou et al 2010). However, owing to its difficulty of cultivation (poor seed viability, virus infection, low and unpredictable yields and slow plant growth rate) (Park et al 2009;Xu and Davey 1983;Zhao et al 2007), low distribution and increasing demand access to this species is very limited and there is a need to develop an effective propagation method using in vitro culture techniques.…”

mentioning

confidence: 99%

Micropropagation of Rehmannia glutinosa Libosch.: production of phenolics and flavonoids and evaluation of antioxidant activity

2014

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Rehmannia glutinosa Libosch., a valuable medicinal plant, was successfully propagated in vitro using shoot tip explants. Shoot multiplication was performed in glass tubes and in a nutrient sprinkle bioreactor. A mixture of 0.1 mg L -1 indole-3-acetic acid (IAA) and 1.0 mg L -1 of 6-benzylaminopurine in Murashige and Skoog (MS) agar-solidified medium proved the best combination for multiple shoot induction, yielding 8.2 shoots per explant after 4 weeks of culture in glass tubes. The number of shoots increased to 21 per explant when the same combination of growth regulators was used in a nutrient sprinkle bioreactor. The shoots rooted with a frequency of 93 % after 6 weeks of culture on MS agar medium supplemented with IAA (0.1 mg L -1 ) before being acclimatized in the greenhouse. The antioxidant activities of methanolic extracts from the leaves and roots of the in vitro-regenerated plants of R. glutinosa cultivated in the greenhouse were evaluated using four in vitro assays: scavenging of free radicals (DPPH and ABTS), transition metal reduction and total antioxidant activity phosphomolybdenum test. In all cases, the methanolic extract from leaves demonstrated better antioxidant activity than those taken from roots. A strong correlation was found between total phenolic and flavonoid content, and the antioxidant capacity of the studied extracts.

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Chloroplast DNA sequencing and detailed microsatellite genotyping of all remnant populations suggests that only a single genet survives of the critically endangered plant Rehmannia japonica

et al. 2016

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Rehmannia japonica (Thunb.) Makino ex T. Yamaz. is an endangered perennial herb species in Japan. Although earlier the Japanese considered it a variety of R. glutinosa, recent Japanese taxonomists have consistently regarded it as an independent species. According to the historical literature, Rehmannia japonica seems to have been known in China and Japan in the past. However, Chinese taxonomists do not recognize R. japonica at present. In Japan, only two populations are known, and although these populations flower every year, seed reproduction has not been observed. In this study, we aimed to reveal the phylogenetic relationships and levels of genetic diversity of R. japonica. A haplotype network based on two chloroplast DNA regions (trnL-trnF and rps16) showed that the sequences of R. japonica were distinguishable by three or four sites of indels from the most closely related species, R. chingii, consistent with the separate species status of R. japonica. An analysis of genetic diversity using twelve microsatellite loci showed that all of the ramets of R. japonica collected from two geographically isolated populations had an identical multilocus genotype, including identical heterozygous genotypes at six loci. This result indicated asexual origin of all sampled ramets. This study also suggests that the absence of sexual reproduction of R. japonica is explained by self-incompatibility combined with only a single genet remaining in the R. japonica populations.

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Genetic diversity of Rehmannia glutinosa cultivars based on sequence-related amplified polymorphism markers

Cited by 11 publications

References 24 publications

SSR Development and Utilization with Rehmannia glutinosa Transcriptome

SSR Development and Utilization with Rehmannia glutinosa Transcriptome

Micropropagation of Rehmannia glutinosa Libosch.: production of phenolics and flavonoids and evaluation of antioxidant activity

Chloroplast DNA sequencing and detailed microsatellite genotyping of all remnant populations suggests that only a single genet survives of the critically endangered plant Rehmannia japonica

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