Genetic Diversity of Ralstonia solanacearum Race 3 in Western Europe determined by AFLP, RC-PFGE and Rep-PCR

Wolf, J. M. Van Der; Bonants, P.J.M.; Smith, J. J.; Hagenaar, M.; Nijhuis, E.H.; Beckhoven, J.R.C.M. van; Saddler, G. S.; Trigalet, André; Feuillade, R.

doi:10.1007/978-3-662-03592-4_6

Cited by 30 publications

(17 citation statements)

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“…Although AFLP clustering was approximately the same as PCR-RFLP clustering, the AFLP procedure was more efficient for assessing intraspecific diversity since it permitted a clearer separation between biovar 2 and N2 and also between biovars 3, 4 and 5. AFLP and PCR-RFLP confirmed that biovar 2 strains are the least genetically diverse of all biovars (Cook et al, 1989 ;Cook & Sequeira, 1994 ;Smith et al, 1995 ;Van der Wolf et al, 1998) ; nevertheless AFLP, in contrast to PCR-RFLP, demonstrated genetic diversity among biovar 2 strains. In contrast, PCR-RFLP permitted strains isolated from musaceous plants to be grouped into two main clusters whereas AFLP grouped them into only one cluster.…”

Section: Clustering Of the Pcr-rflp And Aflp Profilesmentioning

confidence: 83%

“…Secondly, we have used the very powerful DNA fingerprinting technique AFLP (Vos et al, 1995), which allows very fine whole genome analysis. AFLP methodology has already been used to study the diversity of race 3 isolates of R. solanacearum (Van der Wolf et al, 1998) S. POUSSIER and OTHERS with 19 previously sequenced R. solanacearum 16S rRNA gene sequences (Taghavi et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

et al. 2000

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The genetic diversity among strains in a worldwide collection of Ralstonia solanacearum, causal agent of bacterial wilt, was assessed by using three different molecular methods. PCR-RFLP analysis of the hrp gene region was extended from previous studies to include additional strains and showed that five amplicons were produced not only with all R. solanacearum strains but also with strains of the closely related bacteria Pseudomonas syzygii and the blood disease bacterium (BDB). However, the three bacterial taxa could be discriminated by specific restriction profiles. The PCR-RFLP clustering, which agreed with the biovar classification and the geographical origin of strains, was confirmed by AFLP. Moreover, AFLP permitted very fine discrimination between different isolates and was able to differentiate strains that were not distinguishable by PCR-RFLP. AFLP and PCR-RFLP analyses confirmed the results of previous investigations which split the species into two divisions, but revealed a further subdivision. This observation was further supported by 16S rRNA sequence data, which grouped biovar 1 strains originating from the southern part of Africa.

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Section: Clustering Of the Pcr-rflp And Aflp Profilesmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

et al. 2000

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“…The disease causes wilting and death in species mainly from tropical and subtropical areas. In recent years, the pathogen has been found in the temperate climates of Northern and Western Europe ( Van der Wolf et al, 1998). The pathogen infects a wide range of crops, such as potato, tomato, eggplant, pepper, groundnut, banana, and ginger (Elphinstone, 2005;Hayward, 1991).…”

Section: Introductionmentioning

confidence: 99%

QTL Analysis for Resistance to Ralstonia solanacearum in Capsicum Accession ‘LS2341’

Mimura¹,

Kageyama

Minamiyama

et al. 2009

J. Japan. Soc. Hort. Sci.

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A Malaysian pepper accession, 'LS2341' (Capsicum annuum L.) is highly resistant to bacterial wilt (BW) caused by Ralstonia solanacearum E. F. Smith (species complex). Quantitative trait locus (QTL) analysis was performed using a double haploid (DH) population derived from a cross between the susceptible cultivar 'California Wonder' and 'LS2341'. A linkage map was constructed using simple sequence repeats (SSRs) and amplified fragment length polymorphism (AFLP). The map spans 974 cM, and consists of 15 linkage groups (LGs). Growth chamber evaluations of BW resistance detected a QTL on LG 11. This LG corresponds to pepper chromosome 1 (P1). The QTL explained 33% of the resistance derived from 'LS2341', and was named Bw1. An SSR marker, CAMS451 was mapped in the centre of the QTL. Although BW-resistance is thought to be polygenically controlled, use of this linkage marker may improve the efficiency of breeding BW-resistant cultivars.

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“…Infected geranium cuttings have been accidentally introduced to North America and Europe from the highland tropics, although the bacterium has not become established in North America (25,29,40,41,53). R3bv2 most likely originated in the Andes with potato plants, since isolates from around the world are essentially clonal (14,22,39,48). Phylotype II, sequevar 7 (formerly race 1), strains that infect tomato, pepper, and tobacco plants are endemic to the warm temperate and subtropical zones of the southeastern United States, but these have never become established north of the mid-Atlantic states.…”

mentioning

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Moderate Temperature Fluctuations Rapidly Reduce the Viability of Ralstonia solanacearum Race 3, Biovar 2, in Infected Geranium, Tomato, and Potato Plants

Scherf

Milling

Allen

2010

Appl Environ Microbiol

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Most Ralstonia solanacearum strains are tropical plant pathogens, but race 3, biovar 2 (R3bv2), strains can cause bacterial wilt in temperate zones or tropical highlands where other strains cannot. R3bv2 is a quarantine pathogen in North America and Europe because of its potential to damage the potato industry in cooler climates. However, R3bv2 will not become established if it cannot survive temperate winters. Previous experiments showed that in water at 4°C, R3bv2 does not survive as long as native U.S. strains, but R3bv2 remains viable longer than U.S. strains in potato tubers at 4°C. To further investigate the effects of temperature on this high-concern pathogen, we assessed the ability of R3bv2 and a native U.S. strain to survive typical temperate winter temperature cycles of 2 days at 5°C followed by 2 days at ؊10°C. We measured pathogen survival in infected tomato and geranium plants, in infected potato tubers, and in sterile water. The population sizes of both strains declined rapidly under these conditions in all three plant hosts and in sterile water, and no culturable R. solanacearum cells were detected after five to seven temperature cycles in plant tissue. The fluctuations played a critical role in loss of bacterial viability, since at a constant temperature of ؊20°C, both strains could survive in infected geranium tissue for at least 6 months. These results suggest that even when sheltered in infected plant tissue, R3bv2 is unlikely to survive the temperature fluctuations typical of a northern temperate winter.

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Genetic Diversity of Ralstonia solanacearum Race 3 in Western Europe determined by AFLP, RC-PFGE and Rep-PCR

Cited by 30 publications

References 5 publications

Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

QTL Analysis for Resistance to Ralstonia solanacearum in Capsicum Accession ‘LS2341’

Moderate Temperature Fluctuations Rapidly Reduce the Viability of Ralstonia solanacearum Race 3, Biovar 2, in Infected Geranium, Tomato, and Potato Plants

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