Genetic diversity of porcine reproductive and respiratory syndrome virus in Thailand and Southeast Asia from 2008 to 2013

Jantafong, Tippawan; Sangtong, Pradit; Saenglub, Wimontiane; Mungkundar, Chatthapon; Romlamduan, Narin; Lekchareonsuk, Chalermpol; Lekcharoensuk, Porntippa

doi:10.1016/j.vetmic.2015.01.017

Cited by 40 publications

(27 citation statements)

References 26 publications

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“…It provides useful information for vaccine selection and renewal. The similar result was also obtained for ORF5 sequence of those strains [13,14] .…”

Section: Discussionsupporting

confidence: 86%

“…Those HP-PRRSV isolates share high sequence identity and have similar deletions/mutations in difference regions of viral genome such as two deletions in non-structural protein 2, one deletion in the 5'-untranslated region, and one deletion in the 3' untranslated region, and some other point mutations [9] . Recently, several studies on genetic variation and phylogenetic relationship based on major structural genes of PRRSV isolates such as ORF5, ORF7 have been done [10][11][12][13][14] . In this study, the 372-bp complete ORF7 of 36 PRRSV isolated from Vietnam collected during 2008-2012 were sequenced and analyzed with ORF7 sequences of PRRS vaccine viruses as well as its parental virus and other published PRRSV strains.…”

Section: Introductionmentioning

confidence: 99%

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Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Thúy¹,

Thu²,

Son³

et al. 2015

Kafkas Univ Vet Fak Derg

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Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases for the swine industry worldwide. The 372-bp complete nuclear capsid protein (N-protein) encoding gene (ORF7) of 36 field PRRSV isolates from Vietnam collected during 2008-2012 were sequenced and compared with certain vaccines and published strains of PRRSV. The ORF7 nucleotide sequence (nt) similarity and amino acid (aa) identity among 36 strains showed the highest and ranged from 95.1 to 100%. These isolates shared similarities with VR-2332 (nt 91.9-94.3%, aa 92.6-96.7%) and LV (nt 62.7-65.3%, aa 58.5-60.1%). There were higher level of similarity with QN07 (nt 96.2-99.1%, aa 96.7-99.1%) from the 2007 PRRS outbreak in Quang Nam province, CH-1a (nt 93.0-96.2%, aa 92.6-95.9%) isolated in China in 1995 and JXA1 (nt 96.7-99.7%, aa 97.5-100%), the highly pathogenic strain from China isolated in 2006. Six aa mutations located in both variable and conserved regions of N-protein amino acid sequence were detected in most of the 36 isolates and highly pathogenic PRRSV strains in China in comparison to the prototype strain VR-2332. Results of sequence analyses indicated that PRRSVs isolated in Vietnam during 2008-2012 were classified as North American genotype. Phylogenetic tree also clustered those 36 PRRSV isolates, other recently reported Vietnamese strains, highly pathogenic Chinese strains and JXA1-R vaccine strain in the same cluster and separated from the prototype strain (VR-2332) and Ingelvac MLV/Besta vaccine strains. The result on genetic characterization of ORF7 of circulating PRRSV strains may assist the development of effective strategies for monitoring and controlling PRRS in Vietnam. Keywords: Genetic variation of ORF7, North American genotype, Phylogenetic analysis, PRRSV Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni'nin Genetik Analizi

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“…It provides useful information for vaccine selection and renewal. The similar result was also obtained for ORF5 sequence of those strains [13,14] .…”

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Thúy¹,

Thu²,

Son³

et al. 2015

Kafkas Univ Vet Fak Derg

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show abstract

“…Since the first outbreak of HP-PRRSV infection in China in 2006, this disease spread subsequently to South East Asian countries including Vietnam and the Philippines in 2007, in Thailand in 2008, and Cambodia and Laos in 2010(An et al, 2011Ni et al, 2012;Tornimbene et al, 2014;Jantafong et al, 2015). Vaccination is now considered to be one of the main strategies to control the HP-PRRSV.…”

Section: Introductionmentioning

confidence: 99%

Comparison of two genetically distant type 2 porcine reproductive and respiratory syndrome virus (PRRSV) modified live vaccines against Vietnamese highly pathogenic PRRSV

Tien

Park

Choi

et al. 2015

Veterinary Microbiology

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“…Since the discovery of PRRS in mainland China in 1996, the epidemic pathogenic strains have mainly been North American genotypes. However, there have been a variety of subtypes of genotypes, due to the constant variation in PRRSV genomes, bringing huge economic losses to China's pig farming industry and posing a problem for disease prevention and control (Chen et al., ; Fan, Wang, Bai, Zhang, & Jiang, ; Jantafong et al., ; Leng et al., ; Shen et al., ; Shi, Holmes, Brar, & Leung, ; Shi, Mo et al., ; Xie et al., ). Recently, the strain JX2014T2 was isolated by our laboratory from the lung tissues of infected pigs from a pig farm in Jiangxi Province and was subjected to complete full‐length sequence splicing.…”

Section: Discussionmentioning

confidence: 99%

The emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus with additional 120aa deletion in Nsp2 region in Jiangxi, China

Wang

et al. 2018

Transbound Emerg Dis

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Highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS), which emerged in China in 2006, was characterized by high fever, high morbidity and high mortality. The causative agent of the disease was a highly pathogenic variant of porcine reproductive and respiratory syndrome virus (also called HP-PRRSV), which has a discontinuous deletion of 1 + 29 amino acids (aa) in the Nsp2 coding region, compared to classical PRRSV. In 2014, fattened pigs on a pig farm in Jiangxi Province suffered from clinical symptoms of high fever, dyspnoea and death. A PRRSV, termed JX2014T2, was isolated from samples of the dead pigs. Genomic analysis of the isolated PRRSV indicated that the genome of the virus was 14,960 bp in length and belonged to the North American genotype. In the Nsp2-coding region, there was a discontinuous deletion of 1 + 29 aa, similar to HP-PRRSV; however, an additional continuous deletion of 120 amino acids between aa 628 and 747 was found. Further analysis of the pathogenicity of PRRSV JX2014T2 was performed in piglets, and the results indicated that all infected piglets suffered from typical clinical symptoms of PRRS, such as high fever, cough, mental depression, anorexia, dyspnoea and palpebral swelling and died within 15 days postinfection (dpi). This demonstrated that the newly isolated PRRSV JX2014T2 strain containing an additional deletion of 120 aa is highly pathogenic to piglets, suggesting that a highly pathogenic variant with new genetic features is circulating in China.

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Genetic diversity of porcine reproductive and respiratory syndrome virus in Thailand and Southeast Asia from 2008 to 2013

Cited by 40 publications

References 26 publications

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Comparison of two genetically distant type 2 porcine reproductive and respiratory syndrome virus (PRRSV) modified live vaccines against Vietnamese highly pathogenic PRRSV

The emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus with additional 120aa deletion in Nsp2 region in Jiangxi, China

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