Genetic diversity of Pleuronectes yokohamae population revealed by fluorescence microsatellite labeled

Pan, Ting; Zhang, Yan; Gao, Tianxiang; Li, Fenghui

doi:10.1016/j.bse.2014.01.015

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…A similar mechanism of genetic diversity stabilization over time has also been reported in other heavily exploited marine fish species [53,54]. The estimated levels of genetic variability in the fish examined in our study were high and comparable with those reported in many other flounder species [55][56][57][58]. This suggests that the currently observed decline in the body condition indices of the European flounder in the Baltic Sea might not be directly associated with the reduction of the species' genetic variability but rather, with the qualitative changes in the gene pool caused by directional selection pressure associated with environmental changes and intensive fishery exploitation [12].…”

Section: Discussionsupporting

confidence: 90%

Population Genetic Study on the European Flounder (Platichthys flesus) from the Southern Baltic Sea Using SNPs and Microsatellite Markers

Kuciński

Jakubowska-Lehrmann

Góra

et al. 2023

Animals

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The European flounder (Platichthys flesus), which is closely related to the recently discovered Baltic flounder (Platichthys solemdali), is currently the third most commercially fished species in the Baltic Sea. According to the available data from the Polish Fisheries Monitoring Center and fishermen’s observations, the body condition indices of the species in the Baltic Sea have declined in recent years. The aim of the present study was to obtain information on the current patterns of genetic variability and the population structure of the European flounder and to verify whether the Baltic flounder is present in the southern Baltic Sea. Moreover, we aimed to verify whether the observed decline in the body condition indices of the species in the Baltic Sea might be associated with adaptive alterations in its gene pool due to increased fishing pressure. For this purpose, 190 fish were collected from four locations along the central coastline of Poland, i.e., Mechelinki, Władysławowo, the Vistula Lagoon in 2018, and the Słupsk Bank in 2020. The fish were morphologically analyzed and then genetically screened by the application of nineteen microsatellite DNA and two diagnostic SNP markers. The examined European flounder specimens displayed a high level of genetic diversity (PIC = 0.832–0.903, I = 2.579–2.768). A lack of significant genetic differentiation (Fst = 0.004, p > 0.05) was observed in all the examined fish, indicating that the European flounder in the sampled area constitutes a single genetic cluster. A significant deficiency in heterozygotes (Fis = 0.093, p < 0.05) and overall deviations from Hardy–Weinberg expectations (H-WE) were only detected in fish sampled from the Słupsk Bank. The estimated effective population size (Ne) among the sampled fish groups varied from 712 (Słupsk Bank) to 10,115 (Władysławowo and Mechelinki). However, the recorded values of the Garza–Williamson indicator (M = 0.574–0.600) and the lack of significant (p > 0.05) differences in Heq > He under the SMM model did not support the species’ population size changes in the past. The applied SNP markers did not detect the presence of the Baltic flounder among the fish sampled from the studied area. The analysis of an association between biological traits and patterns of genetic diversity did not detect any signs of directional selection or density-dependent adaptive changes in the gene pool of the examined fish that might be caused by increased fishing pressure.

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Section: Discussionsupporting

confidence: 90%

Population Genetic Study on the European Flounder (Platichthys flesus) from the Southern Baltic Sea Using SNPs and Microsatellite Markers

Kuciński

Jakubowska-Lehrmann

Góra

et al. 2023

Animals

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“…Then, the polymorphism of primers was detected by capillary electrophoresis combined with uorescence modi cation, and the EST-SSR loci with high polymorphism were screened. Compared to the screening of polymorphic SSR loci by silver staining, the use of uorescence has many advantages, including simple experimental operation; automatic identi cation and collection of data through software to improve e ciency; and the ability to identify alleles that are only 1-2 bp apart to improve data accuracy(Li et al, 2009;Pan et al, 2014). PIC is an important indicator for measuring the polymorphism information of SSR loci(Wang et al, 2021).…”

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confidence: 99%

Inferring the impact of large-scale aquaculture of Haliotis discus hannai in northern and southern China using EST-SSR markers

杨,

Zhou,

et al. 2024

Preprint

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The Pacific abalone Haliotis discus hannai originated in cold waters, but as an economically important aquaculture shellfish in China, there has been a large-scale transfer of fish to Fujian Province in southern China for culture. Our goal was to clarify the current status of the genetic structure of Pacific abalone in China. In this study, eighteen polymorphic EST-SSR loci were successfully developed based on the hemolymph transcriptome data of Pacific abalone, and thirteen highly polymorphic EST-SSR loci were selected for the genetic variation analysis of six populations collected from Zhangzhou in Fujian Province (ZZ), Dalian in Liaoning Province (DL), Rongcheng in Shandong Province (RC), and the Changshan Archipelago, including Nanhuangcheng Island (NH), Daqin Island (DQ), and Tuoji Island (TJ). The results showed that the average number of observed alleles was 8.0769 (RC)-11.3848 (DQ) in each population. The number of observed alleles in the DQ, NH, and TJ populations was significantly higher than that in the RC population. If the NH and TJ cultured populations of the Changshan Archipelago were excluded, then compared with the DQ wild population, the number of alleles in the cultured population decreased by 22.79%. The pairwise Fst values and analysis of molecular variance (AMOVA) revealed significant population differentiation among most populations, with the RC and ZZ cultured populations exhibiting the largest population differentiation (Fst = 0.1334). The UPGMA phylogenetic tree and structural analysis divided the six populations into two groups (group 1: NH, DQ, and ZZ; group 2: DL, TJ, and RC), and there was no relationship between geographical distance and genetic distance. These results may reflect the large-scale culture from north to south in China and the exchange of juveniles between hatcheries. Different breeding conditions have led to a higher degree of genetic differentiation between the RC and ZZ populations. In conclusion, this study provides valuable information on the genetic structure of Pacific abalone populations in China and contributes to the management and restoration of Pacific abalone.

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Development of EST-SSRs from the ark shell (Scapharca broughtonii) transcriptome and their application in genetic analysis of four populations

Wang

Liu

et al. 2021

Genes Genom

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Genetic diversity of Pleuronectes yokohamae population revealed by fluorescence microsatellite labeled

Cited by 4 publications

References 30 publications

Population Genetic Study on the European Flounder (Platichthys flesus) from the Southern Baltic Sea Using SNPs and Microsatellite Markers

Population Genetic Study on the European Flounder (Platichthys flesus) from the Southern Baltic Sea Using SNPs and Microsatellite Markers

Inferring the impact of large-scale aquaculture of Haliotis discus hannai in northern and southern China using EST-SSR markers

Development of EST-SSRs from the ark shell (Scapharca broughtonii) transcriptome and their application in genetic analysis of four populations

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