Genetic diversity of Plasmodium falciparum isolates from naturally infected children in north-central Nigeria using the merozoite surface protein-2 as molecular marker

Oyedeji, Segun Isaac; Awobode, Henrietta Oluwatoyin; Anumudu, Chiaka I.; Kun, Jürgen F.T.

doi:10.1016/s1995-7645(13)60102-9

Cited by 16 publications

(25 citation statements)

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“…FC27 allelic type has been shown to be more prevalent in asymptomatic rather than symptomatic Plasmodium -infected individuals [24], suggesting a lower risk of developing symptomatic malaria with increasing carriage of isolates belonging to the FC27 allelic family. Such observations are supported by previous studies in Senegal [5, 13] and Nigeria [25] but contrast with data from Benin and Gabon [26, 27], emphasizing the need for multicentric studies using P. falciparum isolates from both asymptomatic and symptomatic individuals to comprehensively establish the potential relationship between specific allele carriage and disease outcome.…”

Section: Discussionmentioning

confidence: 99%

Spatio-temporal analysis of the genetic diversity and complexity of Plasmodium falciparum infections in Kedougou, southeastern Senegal

et al. 2017

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BackgroundGenetic analyses of the malaria parasite population and its temporal and spatial dynamics could provide an assessment of the effectiveness of disease control strategies. The genetic diversity of Plasmodium falciparum has been poorly documented in Senegal, and limited data are available from the Kedougou Region. This study examines the spatial and temporal variation of the genetic diversity and complexity of P. falciparum infections in acute febrile patients in Kedougou, southeastern Senegal. A total of 263 sera from patients presenting with acute febrile illness and attending Kedougou health facilities between July 2009 and July 2013 were obtained from a collection established as part of arbovirus surveillance in Kedougou. Samples identified as P. falciparum by nested PCR were characterized for their genetic diversity and complexity using msp-1 and msp-2 polymorphic markers.ResultsSamples containing only P. falciparum accounted for 60.83% (160/263) of the examined samples. All three msp-1 allelic families (K1, MAD20 and RO33) and two msp-2 allelic families (FC27 and 3D7) were detected in all villages investigated over the 5-year collection period. The average genotype per allelic family was comparable between villages. Frequencies of msp-1 and msp-2 allelic types showed no correlation with age (Fisher’s exact test, P = 0.59) or gender (Fisher’s exact test, P = 0.973), and were similarly distributed throughout the 5-year sampling period (Fisher’s exact test, P = 0.412) and across villages (Fisher’s exact test, P = 0.866). Mean multiplicity of infection (MOI) for both msp-1 and msp-2 was highest in Kedougou village (2.25 and 2.21, respectively) and among younger patients aged ≤ 15 years (2.12 and 2.00, respectively). The mean MOI was highest in 2009 and decreased progressively onward.ConclusionCharacterization of the genetic diversity and complexity of P. falciparum infections in Kedougou revealed no spatio-temporal variation in the genetic diversity of P. falciparum isolates. However, mean MOI varied with time of sera collection and decreased over the course of the study (July 2009 to July 2013). This suggests a slow progressive decrease of malaria transmission intensity in Kedougou Region despite the limited impact of preventive and control measures implemented by the National Malaria Control Programme on malaria morbidity and mortality.

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Section: Discussionmentioning

confidence: 99%

Spatio-temporal analysis of the genetic diversity and complexity of Plasmodium falciparum infections in Kedougou, southeastern Senegal

et al. 2017

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“…The relative importance of hypervariable locus position 197 was calculated using machine-learning random forest analysis 9 , and relative risk ratio models 22 , and was measured directly in this analysis. The effectiveness of a malaria vaccine displaying significant strain-specific efficacy would be limited by simultaneous or sequential infection with multiple parasite clones, which is common [39][40][41] .…”

Section: Discussionmentioning

confidence: 99%

Microarray analyses reveal strain-specific antibody responses to Plasmodium falciparum apical membrane antigen 1 variants following natural infection and vaccination

Bailey

Berry

Travassos

et al. 2020

Sci Rep

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Vaccines based on Plasmodium falciparum apical membrane antigen 1 (AMA1) have failed due to extensive polymorphism in AMA1. To assess the strain-specificity of antibody responses to malaria infection and AMA1 vaccination, we designed protein and peptide microarrays representing hundreds of unique AMA1 variants. Following clinical malaria episodes, children had short-lived, sequenceindependent increases in average whole-protein seroreactivity, as well as strain-specific responses to peptides representing diverse epitopes. Vaccination resulted in dramatically increased seroreactivity to all 263 AMA1 whole-protein variants. High-density peptide analysis revealed that vaccinated children had increases in seroreactivity to four distinct epitopes that exceeded responses to natural infection. A single amino acid change was critical to seroreactivity to peptides in a region of AMA1 associated with strain-specific vaccine efficacy. Antibody measurements using whole antigens may be biased towards conserved, immunodominant epitopes. Peptide microarrays may help to identify immunogenic epitopes, define correlates of vaccine protection, and measure strain-specific vaccine-induced antibodies.Plasmodium falciparum apical membrane antigen 1 (AMA1) is a malaria parasite surface protein involved in red blood cell invasion 1 . AMA1 has been a leading target for vaccine development because of its high antigenicity and the ability of AMA1 antibodies to inhibit parasite growth both in vitro and in non-human primates 2-6 . However, in vitro and molecular epidemiological studies provided early evidence that AMA1 exhibits immune evasion that is both domain-and sequence-specific 7-12 . The AMA1 protein contains a hydrophobic cleft that is the binding site of red blood cell invasion machinery [13][14][15][16] . Synthetic peptides that bind to this hydrophobic cleft prevent interaction with the red blood cell invasion complex, obstructing parasite invasion 17,18 . The hydrophobic cleft is in the

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“…leukocyte count of 8,000 per µl of blood as described Five species of apicomplexan parasites of the earlier (Oyedeji et al, 2013). genus Plasmodium, are acknowledged to cause malaria in humans, namely: Plasmodium falciparum, DNA extraction and PCR ® P. malariae, P. ovale, P. vivax, and P. knowlesi.…”

Section: Materials and Methods Increase In Il-18 Concentrations Has Bmentioning

confidence: 99%

Single nucleotide polymorphism (rs1035130C>T) in the interleukin 18 receptor 1 gene and susceptibility to severe <i>P. falciparum</i> malaria in Lafia, North-central Nigeria

Oyedeji¹,

Awobode²,

Bassi³

et al. 2017

Nig. J. Biotechnol

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The interleukin 18 receptor 1 (IL18R1) gene encodes a potent cytokine receptor that is critical for IL18 binding and subsequent signal transduction. This gene is a member of the IL1 receptor family that resides in a cluster of genes on human chromosome 2. Several polymorphisms have been shown to exist on this gene locus and some were found to be associated with inflammatory diseases but there is paucity of data on association with malaria. This study therefore, was aimed at determining the possible association of the rs1035130C>T coding polymorphism with severe malaria in Lafia, North-central Nigeria. The rs1035130C>T polymorphism was genotyped in a total of 214 participants including 98 severe malaria cases and 116 asymptomatic controls. DNA ® was extracted from blood spotted on filter paper using the QIAamp DNA Mini Kit. The ABI PRISM® 3100 Genetic Analyzer was used to sequence the polymorphic locus. Our data showed a significantly higher frequency (P=0.021) of the CT heterozygous genotype in the asymptomatic control group (25.0%) than in the severe malaria group (13.27%). In addition, the frequency of the minor T allele was significantly higher (P=0.013) in the asymptomatic controls compared to the severe malaria cases. However, the TT homozygous genotype was not found in the severe malaria group. These results suggest a contributory role of the rs1035130C>T polymorphism in regulating host susceptibility or resistance to severe malaria and consequently have implications for understanding the molecular mechanisms of malaria pathogenesis.

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Genetic diversity of Plasmodium falciparum isolates from naturally infected children in north-central Nigeria using the merozoite surface protein-2 as molecular marker

Abstract: This study showed a high genetic diversity of P. falciparum isolates in North-central Nigeria and is comparable to reports from similar areas with high malaria transmission intensity.

Cited by 16 publications

References 47 publications

Spatio-temporal analysis of the genetic diversity and complexity of Plasmodium falciparum infections in Kedougou, southeastern Senegal

Spatio-temporal analysis of the genetic diversity and complexity of Plasmodium falciparum infections in Kedougou, southeastern Senegal

Microarray analyses reveal strain-specific antibody responses to Plasmodium falciparum apical membrane antigen 1 variants following natural infection and vaccination

Single nucleotide polymorphism (rs1035130C>T) in the interleukin 18 receptor 1 gene and susceptibility to severe <i>P. falciparum</i> malaria in Lafia, North-central Nigeria

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