Genetic diversity of Plasmodium falciparum isolates based on MSP-1 and MSP-2 genes from Kolla-Shele area, Arbaminch Zuria District, southwest Ethiopia

Mohammed, Hussein; Mindaye, Tedla; Belayneh, Meseret; Kassa, Moges; Assefa, Ashenafi; Tadesse, Mekonnen; Woyessa, Adugna; Mengesha, T; Kebede, Amha

doi:10.1186/s12936-015-0604-8

Cited by 62 publications

(110 citation statements)

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“…The MOI reported in this study was high compared to those reported from a moderate transmission region in Kolla-Shele Southwest Ethiopia [16], but consistentwith findings from Cote d’Ivoire [30]. Thisisinagreement withprevious observations of an increased MOIwith increase ingendemicity [31].…”

Section: Discussionsupporting

confidence: 90%

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Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

et al. 2017

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BackgroundIn malaria endemic regions, Plasmodium falciparum infection is characterized by extensive genetic diversity. Describing this diversity provides important information about the local malaria situation. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Pawe district, North West Ethiopia, using the highly polymorphic merozoite surface protein 2 gene.MethodsAtotal of 92 isolates from patients with uncomplicated P. falciparum attending Pawe Health Centre were collected from September to December 2013. Genomic DNA was extracted using the Chelex method and analyzed by length polymorphism following gel electrophoresis of DNA products from nested PCR of msp2 (block 3), targeting allelic families of FC27 and 3D7/IC.ResultsThere were twenty-two different msp2 alleles, 11 corresponding to the 3D7/ IC and 11 to the FC27 allelic family. The frequency of isolates of the msp2 3D7/IC allelic familywas higher (51%) compared to FC27 (49%). The majority of the isolates (76%) contained multiple infections andthe overall mean multiplicity of infection was 2.8 (CI 95% 2.55–3.03). The heterozygosity index was 0.66 for msp2. There was no statically significant difference in the multiplicity of infection by age or parasite density.ConclusionsThe results of this study show that P.falciparum polymorphismsare extensive in Northwest Ethiopia and most of the infections are composed of multiple clones.

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Section: Discussionsupporting

confidence: 90%

“…falciparum . To our knowledge this is the second such study, after our previous report from southwest Ethiopia [16]. This study determined the genetic polymorphism of P .…”

Section: Discussionmentioning

confidence: 89%

Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

et al. 2017

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“…Indeed, the numbers of alleles (bands) detected may be underestimated due to sensitivity of the PCR technique used as minor fragments (<50 bp) cannot be detected on the agarose gel. The number of alleles detected for msp-1 and msp-2 were 10 and 19, respectively (Additional file 1: Figure S1 and Additional file 2: Figure S2), which is high compared with the reported values in other areas of Ethiopia [46,50].…”

Section: Discussionmentioning

confidence: 73%

“…Samples from participants with multi-clonal infection produced more than one amplified fragment after either the three msp-1 or the two msp-2 allelic family PCR reactions [46]. Samples from participants with clonal infection produced a single product after the three msp-1 PCR reactions as well as a single product after the two msp-2 reactions.…”

Section: Resultsmentioning

confidence: 91%

Genetic diversity and genotype multiplicity of Plasmodium falciparum infection in patients with uncomplicated malaria in Chewaka district, Ethiopia

Abamecha

El-Abid

Yilma

et al. 2020

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Background: Genetic diversity in Plasmodium falciparum poses a major threat to malaria control and elimination interventions. Characterization of the genetic diversity of Plasmodium falciparum strains can be used to assess intensity of parasite transmission and identify potential deficiencies in malaria control programmes, which provides vital information to evaluating malaria elimination efforts. In this study, we investigated the P. falciparum genetic diversity and genotype multiplicity of infection in parasite isolates from cases with uncomplicated P. falciparum malaria in Southwest Ethiopia.Methods: A total of 80 P. falciparum microscopy and qPCR positive blood samples were collected from study participants aged six months to sixty years, who visited the health facilities during the evaluation of a therapeutic efficacy study of arthemeter-lumefantrine from September-December, 2017. Polymorphic regions of the msp-1 and msp-2 were genotyped by nested polymerase chain reactions (nPCR) followed by gel electrophoresis for fragment analysis.Results: Of 80 qPCR-positive samples analyzed for polymorphisms on msp-1 and msp-2 genes, the efficiency of msp-1 and msp-2 gene amplification reactions with family-specific primers were 95 % and 98.8%, respectively. A total of 29 msp alleles (10 for msp-1and 19 for msp-2) were detected. In msp-1, K1 was the predominant allelic family detected in 47.7% (42/88) of the samples followed by Mad20 and RO33. For msp-2, the frequency of FC27 and IC/3D7 were 77% (57/74) and 76% (56/74), respectively. Eighty percent (80%) of isolates had multiple genotypes and the overall mean multiplicity of infection was 3.2 (95% CI: 2.87- 3.46). The heterozygosity index was 0.43, and 0.85 for msp-1 and msp-2, respectively. There was no significant association between multiplicity of infection and age or parasite density.Conclusions: The study revealed high levels of genetic diversity and mixed-strain infections of P. falciparum populations in Chewaka district, Ethiopia; reflecting both the endemicity level and malaria transmission remained high and more strengthened control efforts are needed in Ethiopia.

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“…Malaria is generally by Plasmodium falciparum, and it is important public health issue of many developing countries [1][2][3][4] . Globally, every year more than 200 million people suffer from the disease and leading to approximately 627,000 deaths.…”

Section: Introduction;mentioning

confidence: 99%

Frequency of Plasmodium Vivax in Patients Having Uncomplicated Malaria.

2019

JMPB

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Background; Malaria which is potentially life threatening disease that is spread by protozoan parasites. Malaria yet remains to be a important public health concern among most of the endemic regions of world. There are 5 most common human species of plasmodium referred as "Plasmodium falciparum, P. vivax, P. ovale, P. knowlesi, and P. malariae" which can spread the disease. The major complications are caused by P. falciparum and P. vivax, with P. falciparum being the more virulent. This study was conducted to determine the frequency of plasmodium falciparum among patients with uncomplicated malaria in our population. Objective; To determine the frequency of Plasmodium vivax in patients with uncomplicated malaria. Study Design; Descriptive, Cross-sectional study. Setting; Department of Medicine, Nishtar Hospital, Multan. Results; Of these study cases, 93 (56.4 %) were male patients while 72 (43.6%) were female patients. Mean age of our study cases was noted to be 35.52 ± 10.39 years. Majority of our study cases i.e. 101 (61.2%) were from rural areas while 64 (38.8%) were from urban areas, 129 (78.2%) were poor and 65 (39.4%) had positive history of using anti -malarial drugs. Mean duration of disease was 4.37 ± 2.82 days and majority of our patients i.e. 107 (64.8%) had disease duration up to 5 days. Plasmodium vivax was diagnosed in 129 (78.2%) patients. Conclusion; High frequency of plasmodium vivax was noted in our study, among patients with uncomplicated malaria. Plasmodium vivax was significantly associated with female gender and previous use of anti-malarial drugs. 43 malarial drugs. Mean duration of disease was 4.37 ± 2.82 days and majority of our patients i.e. 107 (64.8%) had disease duration up to 5 days. A study conducted in KPK by Shah et al 21 has also documented similar findings.Plasmodium vivax was diagnosed in 129 (78.2%) of our study cases. A study conducted by Khattak et al 18 also reported 86 % cases with malaria were diagnosed with plasmodium vivax which is in compliance with that of our study results. A study conducted by Leghari et al 19 in Bahawalpur has also reported plasmodium vivax being more prevalent causative agent which is in compliance with our study results. Yasinzai et al 20 reported plasmodium falciparum in 70 % malaria patients which is different from our study results. In another study by Yasinzai et al 15 ,in Qallat district while P. falciparum was 25 % which is close to our study results. Fazal et al 13 reported plasmodium vivax 87.8% from Mardan. Conclusion;High frequency of plasmodium vivax was noted in our study, among patients with uncomplicated malaria. Plasmodium falciparum was significantly associated with female gender and previous use of anti-malarial drugs.

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Genetic diversity of Plasmodium falciparum isolates based on MSP-1 and MSP-2 genes from Kolla-Shele area, Arbaminch Zuria District, southwest Ethiopia

Cited by 62 publications

References 26 publications

Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

Genetic diversity and genotype multiplicity of Plasmodium falciparum infection in patients with uncomplicated malaria in Chewaka district, Ethiopia

Frequency of Plasmodium Vivax in Patients Having Uncomplicated Malaria.

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