Genetic diversity of pinto and fresh bean (Phaseolusvulgaris L.) germplasm collected from Erzincan province of Turkey by inter-primer binding site (iPBS) retrotransposon markers

Abstract

In this study, genetic diversity among 71 bean genotypes (41 pinto beans and 30 fresh bean genotypes) and 4 commercial cultivars (Aleyna, Gina, Perolar, and Serra) commonly grown at Erzincan province in Turkey were investigated. The iPBS (inter-primer binding site) markers were used to investigate the genetic diversity of the genotypes. Polymorphism ratio was identified as 100% in all primers. Twenty-seven primers were studied and number of alleles in primers varied between 13-69 (with an average value of 37.14). According to cluster analysis based on iPBS data, genotypes were divided into 2 groups. Polymorphic information content (PIC) values varied between 0.19 (iPBS 2077) and 0.42 (iPBS 2381, iPBS 2385, and iPBS 2389) with an average PIC value of 0.33. According to genetic structure analysis, genotypes were divided into 2 subpopulations. Majority of the genotypes of the regions from where beans were collected were placed in the same groups.

iPBS-Retrotransposon Markers in the Analysis of Genetic Diversity among Common Bean (Phaseolus vulgaris L.) Germplasm from Türkiye

“…The plants were grown for tissue sampling in the greenhouse of Atatürk University, Department of Field Crops, Faculty of Agriculture. to their universality [26]. The universality of the iPBS-retrotransposon marker has been proven and molecular characterization and phylogenetic studies are available for these markers, also in beans [8,24,26].…”

“…Due to limitations in both LTR and non-LTR retrotransposons, inter primer binding site (iPBS) retrotransposons have been developed as a universal marker used in the characterization of both animal and plant species [ 25 ]. iPBS markers are the dominant markers and have become a preferred marker in genetic diversity assessment in recent years due to their universality [ 26 ]. The universality of the iPBS-retrotransposon marker has been proven and molecular characterization and phylogenetic studies are available for these markers, also in beans [ 8 , 24 , 26 ].…”

“…iPBS markers are the dominant markers and have become a preferred marker in genetic diversity assessment in recent years due to their universality [ 26 ]. The universality of the iPBS-retrotransposon marker has been proven and molecular characterization and phylogenetic studies are available for these markers, also in beans [ 8 , 24 , 26 ]. In our previous studies [ 26 ] and in the studies of other researchers [ 8 , 9 ], it has been observed that retrotransposon markers are quite efficient for genetic diversity studies in terms of the total number of amplified and polymorphic bands.…”

“…The universality of the iPBS-retrotransposon marker has been proven and molecular characterization and phylogenetic studies are available for these markers, also in beans [ 8 , 24 , 26 ]. In our previous studies [ 26 ] and in the studies of other researchers [ 8 , 9 ], it has been observed that retrotransposon markers are quite efficient for genetic diversity studies in terms of the total number of amplified and polymorphic bands. The local varieties evaluated so far represent only a small subset of the available resources.…”

Bazı Gernik Buğdayların iPBS Markörleri Kullanılarak Moleküler Karakterizasyonu

“…Several molecular marker systems have been used for determine genetic variation in different cereals such as rice, chickpea, lentil, barley, wheat and oat (Tarang et al, 2020;Andeden et al, 2013;Khazaei et al, 2016;Hou et al, 2005;Gurcan et al, 2017;Boczkowska et al, 2014). Recently, iPBS molecular markers developed based on retrotransposons (RTNs) have been used by several researchers for determination of genetic diversity in various plant species (Yıldız et al, 2015;Sipahi and Yumurtacı, 2020;Shirmohammadli et al, 2018;Öztürk et al, 2020;Karık et al, 2019;Hossein-Pour et al, 2019;Borno et al, 2017;Ali et al, 2019). Inter-primer binding site (iPBS) markers based on retrotransposon have advantages compared with other retrotransposon markers because of universal markers depends upon the presence of tRNA as a reverse transcriptase primer binding site, cost-effective and high efficiency (Kalendar et al, 2010;Nadeem et al, 2018).…”