Genetic Diversity of Pectobacterium carotovorum subsp. brasiliensis Isolated in Korea

Lee, Dong Hwan; Kim, Jin-Beom; Lim, Joong‐Soo; Han, Sang-Wook; Heu, Sunggi

doi:10.5423/ppj.oa.12.2013.0117

Cited by 52 publications

(51 citation statements)

References 27 publications

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“…PCR amplification failed to amplify the car F and car G genes in Pcb (XT3, XT10, G4P5 and G4P7) (results not shown), however, because of the possibility of sequence variation between closely related bacterial strains, one cannot rule out the presence or absence of a gene in a given bacterial genome solely based of PCR. Furthermore, because of the genetic heterogeneity of Pcb strains and SREs in general, as seen by the recent taxonomic reclassification of several SREs (34, 78), it is possible that the genome sequences of Pcb strains XT3, XT10, G4P5 and G4P7 do not encode homologs of CarF and CarG, or do not encode the cognate CarF and CarG homologs providing resistance to carbapenem produced by Pcb 1692. Alternatively, if present, the carF and G genes of Pcb strains XT3, XT10, G4P5 and G4P7may not be transcribed if these strains have a truncated car gene cluster similar the ones described in our comparative analysis.…”

Section: Discussionmentioning

confidence: 99%

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

Nkomo

et al. 2018

Preprint

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17Plant microbial communities' complexity provide a rich model for investigation on biochemical and 18 regulatory strategies involved in interbacterial competition. Within these niches, the soft rot 19 Enterobacteriaceae (SRE) comprise an emerging group of plant-pathogens inflicting soft rot/black-20 leg diseases and causing economic losses worldwide in a variety of crops. In this report, a range of 21 molecular and computational techniques are utilized to survey the contribution of antimicrobial 22Pcb1692 inhibits growth of other SRE and several potato endophytes using either the type VI 51 secretion, carbapenem or bacteriocins. Carbapenem plays a role in both inter and intrabacterial 52 competition in vitro, while the Pcb1692T6SS plays a role in interbacterial competition in planta (in 53 potato tubers). We also demonstrate that carbapenem regulation requires the presence of 54 environmental iron and oxygen in a complex network consisting of Pcb1692 Fur, SlyA, and ExpI. 55

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Section: Discussionmentioning

confidence: 99%

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

Nkomo

et al. 2018

Preprint

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“…P. wasabie and P. carotovurum ssp. brasilensis are also found to infect potato in several regions worldwide (Waleron et al, 2013; Lee et al, 2014). In Europe, Dickeya dianthicola is reported to be very important in potato disease, although more recently, a new Dickeya species called D. solani is being more frequently identified.…”

Section: Phytopathogens Targeted For Phage Biocontrol and How They Armentioning

confidence: 99%

Bacteriophages and Bacterial Plant Diseases

et al. 2017

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Losses in crop yields due to disease need to be reduced in order to meet increasing global food demands associated with growth in the human population. There is a well-recognized need to develop new environmentally friendly control strategies to combat bacterial crop disease. Current control measures involving the use of traditional chemicals or antibiotics are losing their efficacy due to the natural development of bacterial resistance to these agents. In addition, there is an increasing awareness that their use is environmentally unfriendly. Bacteriophages, the viruses of bacteria, have received increased research interest in recent years as a realistic environmentally friendly means of controlling bacterial diseases. Their use presents a viable control measure for a number of destructive bacterial crop diseases, with some phage-based products already becoming available on the market. Phage biocontrol possesses advantages over chemical controls in that tailor-made phage cocktails can be adapted to target specific disease-causing bacteria. Unlike chemical control measures, phage mixtures can be easily adapted for bacterial resistance which may develop over time. In this review, we will examine the progress and challenges for phage-based disease biocontrol in food crops.

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“…fastidiosa, allowing systemic expansion by the pathogen (Sun et al, 2011). Extracellular degradative enzymes are structurally and functionally diverse, and include proteases, cellulases, pectinases and xylanases (Dejean et al, 2013;Lee et al, 2014;Toth et al, 2003). These cell wall-degrading enzymes are particularly associated with Phytoplasma, Xylella and Xanthomonas spp., and soft-rot pathogens from the genera Erwinia and Pectobacterium.…”

Section: Getting Inside the Plant-stomatal Hijacking And Cell Wall-damentioning

confidence: 99%

Bacterial pathogenesis of plants: future challenges from a microbial perspective

Pfeilmeier

Caly

Malone

2016

Molecular Plant Pathology

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Summary Future Challenges in Plant Pathology Plant infection is a complicated process. On encountering a plant, pathogenic microorganisms must first adapt to life on the epiphytic surface, and survive long enough to initiate an infection. Responsiveness to the environment is critical throughout infection, with intracellular and community‐level signal transduction pathways integrating environmental signals and triggering appropriate responses in the bacterial population. Ultimately, phytopathogens must migrate from the epiphytic surface into the plant tissue using motility and chemotaxis pathways. This migration is coupled with overcoming the physical and chemical barriers to entry into the plant apoplast. Once inside the plant, bacteria use an array of secretion systems to release phytotoxins and protein effectors that fulfil diverse pathogenic functions (Fig. 1 ) (Melotto and Kunkel, 2013 ; Phan Tran et al ., 2011 ). As our understanding of the pathways and mechanisms underpinning plant pathogenicity increases, a number of central research challenges are emerging that will profoundly shape the direction of research in the future. We need to understand the bacterial phenotypes that promote epiphytic survival and surface adaptation in pathogenic bacteria. How do these pathways function in the context of the plant‐associated microbiome, and what impact does this complex microbial community have on the onset and severity of plant infections? The huge importance of bacterial signal transduction to every stage of plant infection is becoming increasingly clear. However, there is a great deal to learn about how these signalling pathways function in phytopathogenic bacteria, and the contribution they make to various aspects of plant pathogenicity. We are increasingly able to explore the structural and functional diversity of small‐molecule natural products from plant pathogens. We need to acquire a much better understanding of the production, deployment, functional redundancy and physiological roles of these molecules. Type III secretion systems (T3SSs) are important and well‐studied contributors to bacterial disease. Several key unanswered questions will shape future investigations of these systems. We need to define the mechanism of hierarchical and temporal control of effector secretion. For successful infection, effectors need to interact with host components to exert their function. Advanced biochemical, proteomic and cell biological techniques will enable us to study the function of effectors inside the host cell in more detail and on a broader scale. Population genomics analyses provide insight into evolutionary adaptation processes of phytopathogens. The determination of the diversity and distribution of type III effectors (T3Es) and other virulence genes wi...

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Genetic Diversity of Pectobacterium carotovorum subsp. brasiliensis Isolated in Korea

Cited by 52 publications

References 27 publications

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

The impact of type VI secretion system, bacteriocins and antibiotics on competition amongst Soft-RotEnterobacteriaceae: Regulation of carbapenem biosynthesis by iron and the transcriptional regulator Fur

Bacteriophages and Bacterial Plant Diseases

Bacterial pathogenesis of plants: future challenges from a microbial perspective

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