Genetic diversity of Orientia tsutsugamushi strains from patients in north India

Kumar, Abhay; Biswal, Manisha; Zaman, Kamran; Sharma, Navneet; Suri, Vikas; Bhalla, Ashish

doi:10.1016/j.ijid.2019.04.030

Cited by 18 publications

(12 citation statements)

References 17 publications

(23 reference statements)

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“…Here, the possibility of one strain outgrowing other in cell culture is non-deniable. In accordance with the previous report (Varghese et al, 2013(Varghese et al, , 2015Chunchanur et al, 2019;Kumar et al, 2019;Kannan et al, 2020;Behera et al, 2021), our study confirms the prevalence of Gilliam followed by Kato-and Karp-like strains. The prevalence of Gilliam strain was observed in four districts of UP, namely, Kushinagar, Gorakhpur, Basti, and Siddharthnagar.…”

Section: Discussionsupporting

confidence: 93%

“…A subsequent study found out the existence of antibodies against OT during AES epidemic period (July–November 2016) as well as during the lean period (December–June 2016), antibody titer being higher during the epidemic period ( Kamble et al, 2020 ). Apart from these studies, various other reports were based on either serological or molecular evidences but none involved isolation of pathogen in cell culture that is a definite method for diagnosis of any disease of unknown etiology ( Janardhanan et al, 2014 ; Varghese et al, 2015 ; Jain et al, 2017 ; Chunchanur et al, 2019 ; Kumar et al, 2019 ; Gupte et al, 2020 ; Kannan et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Genotypic Characterization of Orientia tsutsugamushi Isolated From Acute Encephalitis Syndrome and Acute Febrile Illness Cases in the Gorakhpur Area, Uttar Pradesh, India

Nanaware

Desai²,

Banerjee³

et al. 2022

Front. Microbiol.

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Scrub typhus infections caused by Orientiatsutsugamushi (OT), continue to remain underdiagnosed globally, due to the lack of distinctive symptoms. The elusive nature of the Acute Encephalitis Syndrome (AES) outbreak in Gorakhpur, Uttar Pradesh that claimed numerous pediatric lives was the driving force of this study which involved serological diagnosis (IgM–ELISA), isolation of OT in cell culture, confirmation by PCR, and characterization by Sanger sequencing. In total, 12 out of 36 patients were seropositive, of which 4 were positive by PCR. Upon enrichment in cell culture, additional 3 patients (including two seronegative) were detected positive by PCR. In total, three of these 7 patients were found to be infected with two strains of OT. Taken together, this study for the first time reports the occurrence of dual infections in addition to three circulating OT genotypes (Gilliam, Kato, and Karp-like) and highlights the significance of enriching OT in cell culture systems for efficient molecular detection.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Genotypic Characterization of Orientia tsutsugamushi Isolated From Acute Encephalitis Syndrome and Acute Febrile Illness Cases in the Gorakhpur Area, Uttar Pradesh, India

Nanaware

Desai²,

Banerjee³

et al. 2022

Front. Microbiol.

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“…44 Molecular characterization of Orientia species was done using PCR amplification of partial 56-kDa, 47-kDa and 16SrRNA genes. Kumar et al (2019) 45 reported genetic diversity of Orientia tsutsugamushi strains in north India covering different states such as Punjab, Haryana, Himachal Pradesh, Uttar Pradesh and Jammu and Kashmir. Molecular characterization of scrub typhus causative agent was done by nested PCR amplification of 483bp region of the 56-kDa antigen gene.…”

Section: O Tsutsugamushi In Indiamentioning

confidence: 99%

Volume 21, Issue 3

2021

Sci Vis

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“…This vital information will help strategize preventive/containment measures and treatment protocols in areas with virulent circulating strains and in turn ensure reduction in morbidity and mortality in such hot spots. As on date, very limited studies have been undertaken to study the antigenic distribution in our country, [18][19][20][21][22] more studies are required for the preparation of an antigenic map of O tsutsugamushi for India. The reports indicate the variable distribution of O tsutsugamushi strains in the country but the recent studies demonstrate the predominance of Karp like strains followed by Gilliam like strains in India along with reports of the presence of other strains as well.…”

Section: Special Issue On XIII Annual Conference Of Ismocdmentioning

confidence: 99%

Scrub Typhus in India: A Critical Commentary

Tilak

2020

JCD

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Scrub typhus in India, although, dates back to World War II, was nearly forgotten barring occasional reporting of outbreaks from the Indian Armed Forces, which kept the interest alive in the disease. Scrub typhus at that time was considered a formidable foe. It was credited with inflicting heavy casualties amongst troops in the China-Burma-India operational corridor, which forced the commanders to withdraw troops from these areas. This tended to alter the course of the war itself. An old foe indeed; scrub typhus continues to take a heavy toll on the vulnerable Armed Forces personnel who are posted in areas that enhance their vulnerability to the disease and has predictably also impacted the unsuspecting civil population too who are residing in such areas. Scrub typhus has undoubtedly emerged as a major public health challenge in India. Slow, yet steady, appreciation of the impact of scrub typhus though is being felt, however, professional efforts are still dismal and have failed to bring the disease to limelight and contain its insistent march. Scrub typhus, although an old foe, has reemerged albeit with new challenges.

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Genetic diversity of Orientia tsutsugamushi strains from patients in north India

Cited by 18 publications

References 17 publications

Genotypic Characterization of Orientia tsutsugamushi Isolated From Acute Encephalitis Syndrome and Acute Febrile Illness Cases in the Gorakhpur Area, Uttar Pradesh, India

Genotypic Characterization of Orientia tsutsugamushi Isolated From Acute Encephalitis Syndrome and Acute Febrile Illness Cases in the Gorakhpur Area, Uttar Pradesh, India

Volume 21, Issue 3

Scrub Typhus in India: A Critical Commentary

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