Genetic Diversity of Opuntia Cultivars Using PCR-RFLP Analysis Based on Fruitfull Gene

Valadez-Moctezuma, Ernestina; Samah, Samir; Santiago-Santiago, D.G.; Pérez-Martínez, Enrique; Gómez‐Sánchez, Rubén; Maldonado-Gómez, J.; Montes-Vázquez, V.

doi:10.17660/actahortic.2015.1067.48

Cited by 2 publications

(1 citation statement)

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“…Nowadays, studies on genetic diversity, awareness on identification of cactus pear genotypes, establishing cactus pear germplasm collection in order to breed new high quality cactus pear variety are increased. Studies related to the genetic diversity of the genus Opuntia have already been conducted, using chloroplast simple sequence repeats (cpSSR) and amplified fragment length polymorphism (AFLPs) (Labra et al 2003), nuclear ribosomal internal transcribed spacer (nrITS) (Griffith, 2004;Srikanth and Whang, 2015), RAPDs (Zoghlami et al 2007;, inter simple sequence repeats (ISSRs) (Valadez-Moctezuma et al 2015a;Ganopoulos et al 2015), microsatellites (Caruso et al 2010;Bendhifi-Zarroug et al 2015;Samah et al 2016) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (Valadez-Moctezuma et al 2015b). The present study is the first molecular research over Opuntia spp.…”

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confidence: 99%

Molecular Characterization of Turkish Cactus Pear (Opuntia spp.) by RAPD Markers

Tütüncü¹,

SIMSEK²,

KAÇAR³

et al. 2016

J PROF ASSOC CACTUS

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Opuntia ficus-indica Mill. commonly known as cactus pear is the most agronomicallyimportant species in Cactaceae. Turkey has important genetic resources of Opuntia spp.which should be characterized for further breeding strategies. In this study, molecularcharacterization of plant materials collected from different regions of Turkey in whichOpuntia species grown naturally, was performed by using Random Amplified PolymorphicDNA (RAPD) markers. DNA was successfully amplified by 50 RAPD primers. Among 250bands generated by the RAPD primers, 180 were polymorphic. The number of bandsdetected by a single primer set ranged from one to 12 (average of five bands/primer). Thepercentage of polymorphism was 72% based on RAPD data. All data were scored asdiscrete characters and unweighted pair group method with arithmetic mean (UPGMA)dendrogram and principle coordinate analysis (PCoA) were performed. Based on theresults, Opuntia genotypes showed high genetic diversity and we showed that RAPDmarkers are powerful tool to discriminate Turkish Opuntia genotypes. The high geneticdiversity existing in the Turkish germplasm suggests that it would be beneficial to utilizethis pool in Opuntia breeding programs and germplasm management activities.

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