Genetic Diversity of Oat Genotypes Using SCoT Markers

Chňapek, Milan; Mikolášová, Lucia; Vivodík, Martin; Gálová, Zdenka; Hromadová, Zuzana; Ražná, Katarí­na; Balážová, Želmí­ra

doi:10.3390/iecps2021-11926

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…All the primers, except SCoT-40, successfully distinguished the control (untreated) treatments of both cultivars of C. roseus L., i.e., victory carmine and local variety. SCoT markers approved their ability to fingerprint in many crops, including Artemisia herba-alba (Omar et al, 2015), barley (Habiba et al, 2021), oat (Chnapek et al, 2022), Thymus (Alqahtani et al, 2020, and maize (Al-Tamimi, 2020). Total data for analysis of 10 SCoT primers indicated that the highest molecular size was 2488 bp produced by primer SCoT-54, whereas the lowest was 143 bp produced by primer SCoT-12.…”

Section: Resultsmentioning

confidence: 99%

Physical and Chemical Mutagenesis in Catharanthus Roseus Plant Using Scot Markers

AL-HAIDARI¹,

AL-TAMIMI²

2023

SABRAOJBG

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The current study comprising molecular identification of the mutagenic effects of gamma rays and sodium azide on Catharanthus roseus using scot markers took place at the Department of Biology, University of Kufa, Iraq. The seeds of two cultivars, i.e., ‘victory carmine’ and a local variety (pink variety) of Catharanthus roseus L (carmine var.) received irradiation with two doses of gamma rays (50 Gray and 75 Gray). Afterward, these got soaked in sodium azide with four concentrations (0.2%, 0.4%, 0.6%, and 0.8%) to induce the genetic variation in the cultivar. The non-treated seeds served as control treatments for comparison with treated ones. Accomplishing irradiation was at an average of 18 Gy/h using Cobalt-60. The effects of gamma rays and sodium azide treatments’ evaluation were through 10 start codon targeted (SCoT) DNA markers. The higher number of distinctive fingerprints was three produced by primers SCoT-26, while primer SCoT-60 provided a distinct fingerprint for only one treatment. However, primer SCoT-40 failed to recognize any treatment with a distinctive fingerprint. The highest molecular size (2488 bp) came from primer SCoT-54, whereas the lower molecular size (143 bp) resulted in primer SCoT-12. Primer SCoT-33 provided the highest value for chief and unique bands, reaching 33 and 18, respectively. The highest number of amplified bands was 77 bands in primer SCoT-54. Primer SCoT-60 produced the highest value for monomorphic bands, primer SCoT-12 gave the highest value for polymorphic bands, polymorphism, and discriminatory (15, 68.18, and 20.83, respectively), and primer SCoT-9 produced the highest value for efficiency.

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Section: Resultsmentioning

confidence: 99%

Physical and Chemical Mutagenesis in Catharanthus Roseus Plant Using Scot Markers

AL-HAIDARI¹,

AL-TAMIMI²

2023

SABRAOJBG

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Molecular genetic diversity among Iranian Petroselinum crispum (Mill.) Nym. ex A.W. Hill germplasms: an ecological overview

Talebi,

Mahdieh,

Ghorbani

et al. 2023

Genet Resour Crop Evol

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Plant Genetic Diversity Studies: Insights from DNA Marker Analyses

Bidyananda,

Jamir,

Nowakowska

et al. 2024

IJPB

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The plant adaptation response to a changing environment depends on the genetic diversity level it possesses. Genetic diversity and a thorough understanding of population indices are pivotal for decoding plant adaptation to dynamic environmental stressors. The development of polymerase chain reaction (PCR)-based molecular markers enables comprehensive population analyses and the precise detection of individuals and groups with unique genetic variations. Various molecular markers have been employed to assess genetic diversity, examine population structure, and delineate cluster patterns within and among populations. DNA markers revolutionize plant diversity studies by allowing detailed analyses of genetic variations, including economically significant trait-influencing genes. Despite their simplicity, they offer high reproducibility, ensuring accurate estimations of plant variation. Integrating multiple marker systems with advanced high-throughput sequencing techniques is poised to enhance the understanding and management of depleting plant genetic resources by providing a comprehensive picture of diversity at the genome-wide level. This review explores diverse molecular markers, elucidating their advantages and limitations, and highlights their impact on evaluating the genetic diversity and population structure of plants.

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Genetic Diversity of Oat Genotypes Using SCoT Markers

Cited by 3 publications

References 12 publications

Physical and Chemical Mutagenesis in Catharanthus Roseus Plant Using Scot Markers

Physical and Chemical Mutagenesis in Catharanthus Roseus Plant Using Scot Markers

Molecular genetic diversity among Iranian Petroselinum crispum (Mill.) Nym. ex A.W. Hill germplasms: an ecological overview

Plant Genetic Diversity Studies: Insights from DNA Marker Analyses

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