Genetic diversity of nuclear ITS1–5.8S–ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East

Tatonova, Yulia V.; Челомина, Г. Н.; Besprosvannykh, Vladimir V.

doi:10.1016/j.parint.2012.07.005

Cited by 46 publications

(47 citation statements)

References 56 publications

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“…Of the 19 haplotypes found in this population, there were at least three major haplotypes that were considerably distinct from each other [50]. Two distinct phylogenetic ITS1 clades were found in an other Opisthorchis species, C. sinensis, in its Russian Far East population [55].…”

Section: Low Genetic Diversity and Pronounced Star-like Haplotype Netmentioning

confidence: 73%

“…The specific choice of ITS1 rather than ITS2 sequence was based on the fact that it is the only region of rDNA which is variable enough to discriminate polymorphisms within species (even though this polymorphism is quite low) [49,55].…”

Section: Choice Of Dna Markersmentioning

confidence: 99%

“…Studies of other Eurasian trematode species with similar reproductive cycle reported significantly higher genetic diversity [39][40][41]50,55]. Notably, European F. hepatica populations showed extensive genetic mtDNA diversity even in local studies (Ireland and the Netherlands) [37,38].…”

Section: Low Genetic Diversity and Pronounced Star-like Haplotype Netmentioning

confidence: 99%

“…Unfortunately, no extensive research on the population genetics of C. sinensis has been published to date. Nevertheless, the genetic diversity study of C. sinensis population from the Russian Far East showed two levels of intraspecific variation for ITS1 sequences [55].…”

Section: Introductionmentioning

confidence: 96%

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Low Genetic Diversity in Wide-Spread Eurasian Liver Fluke Opisthorchis felineus Suggests Special Demographic History of This Trematode Species

2017

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Opisthorchis felineus or Siberian liver fluke is a trematode parasite (Opisthorchiidae) that infects the hepato-biliary system of humans and other mammals. Despite its public health significance, this wide-spread Eurasian species is one of the most poorly studied human liver flukes and nothing is known about its population genetic structure and demographic history. In this paper, we attempt to fill this gap for the first time and to explore the genetic diversity in O. felineus populations from Eastern Europe (Ukraine, European part of Russia), Northern Asia (Siberia) and Central Asia (Northern Kazakhstan). Analysis of marker DNA fragments from O. felineus mitochondrial cytochrome c oxidase subunit 1 and 3 (cox1, cox3) and nuclear rDNA internal transcribed spacer 1 (ITS1) sequences revealed that genetic diversity is very low across the large geographic range of this species. Microevolutionary processes in populations of trematodes may well be influenced by their peculiar biology. Nevertheless, we suggest that lack of population genetics structure observed in O. felineus can be primarily explained by the Pleistocene glacial events and subsequent sudden population growth from a very limited group of founders. Rapid range expansion of O. felineus through Asian and European territories after severe bottleneck points to a high dispersal potential of this trematode species.

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Section: Low Genetic Diversity and Pronounced Star-like Haplotype Netmentioning

confidence: 73%

Section: Choice Of Dna Markersmentioning

confidence: 99%

Section: Low Genetic Diversity and Pronounced Star-like Haplotype Netmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Low Genetic Diversity in Wide-Spread Eurasian Liver Fluke Opisthorchis felineus Suggests Special Demographic History of This Trematode Species

2017

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“…This assay was the confi rmatory tool to assess the infection and molecular epidemiological examination of parasite. Tatonova et al (2012) reported the molecular identifi cation and sequence difference in Clonorchis sinensis from Russia using ITS-1, 5.8S and ITS-2 region. The fi ndings expressed the 100 % homology for all 5.8S and ITS-2 sequences while two levels of intra-specifi c differences were observed in complete ITS-1 region.…”

Section: Opisthorchiidae (Looss 1899)mentioning

confidence: 99%

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

et al. 2015

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SummaryThe rDNA region of eukaryotes has the immense potential to resolve the evolutionary and phylogeny problems using molecular markers. As evident from the present review, ITS region data is considered for interpretation of inter and intra-specifi c variations of 136 studies of 33 families including 78 genus and 114 species affecting individuals worldwide. Along with ITS-1 and ITS-2 region in 29 studies 18S region, in 38 studies 28S region and in 43 studies mitochondrial genes (COI and NDI) were also analyzed. Three new genera (Allobilharzia gen. nov., Caulanus gen. nov., and Latuterus gen. nov.) and 49 new species were discovered. Only 47 studies expressed variations at intra-specifi c and inter-specifi c level in complete ITS region, ITS-1 and ITS-2 rDNA sequences due to differences in nucleotide positions. According to the fi ndings ITS region is more reliable and precise marker for demarcation and identifi cation of species in combination of other DNA markers. Major studies were involved around the parasites of families Fasciolidae, Schistosomatidae, Opisthorchidae, Paragonimidae and Paramphistomidae, Clinostomidae, Diplostomidae, Haploporidae, among others infecting humans, farm animals, birds, fi shes, reptiles and amphibians on the clinical basis. In future, molecular and bioinformatics aspects based on genetic variations will lead to explore the untouched areas of trematodes.

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Liver Flukes: Clonorchis and Opisthorchis

Saijuntha

Sithithaworn

Kaitsopit

et al. 2014

Advances in Experimental Medicine and Biology

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Genetic diversity of nuclear ITS1–5.8S–ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East

Cited by 46 publications

References 56 publications

Low Genetic Diversity in Wide-Spread Eurasian Liver Fluke Opisthorchis felineus Suggests Special Demographic History of This Trematode Species

Low Genetic Diversity in Wide-Spread Eurasian Liver Fluke Opisthorchis felineus Suggests Special Demographic History of This Trematode Species

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

Liver Flukes: Clonorchis and Opisthorchis

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