Genetic diversity of nine faba bean (Vicia faba L.) populations revealed by isozyme markers

Ouji, Ali; Suso, M. J.; Rouaissi, Mustapha; Abdellaoui, Raoudha; Gazzah, Mohamed El

doi:10.1007/s13258-010-0070-1

Cited by 16 publications

(15 citation statements)

References 32 publications

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“…Interestingly, our data are consistent with previous studies in faba bean which revealed a larger genetic variation and differentiation within than between population (Terzopoulos and Bebeli 2008; Ouji et al 2011;Oliveira et al 2016). Moreover, this was consistent with the findings from other species such as grapevine (De Andrés et al 2012), olive (Belaj et al 2007), apple ) and lettuce (Rauscher and Simko 2013).…”

Section: Discussionsupporting

confidence: 93%

“…Morphological traits (Terzopoulos et al 2004;Yahia et al 2012), isozyme markers (Ouji et al 2011) and molecular markers such as RAPD (Link et al 1995), SSAP (Ouji et al 2012), AFLP (Zied et al 2003), SRAP (Alghamdi et al 2012), and ISSR (Wang et al 2012) have been used for detection of genetic diversity in faba bean germplasm. In the other hand, SSRs have been widely applied in plant genetics and breeding, because they are codominant, easy to score, and highly abundant.…”

Section: Introductionmentioning

confidence: 99%

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Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Rebaa¹,

Abid²,

Aouida³

et al. 2017

Physiol Mol Biol Plants

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The genetic diversity of 21 faba bean populations was examined using morphological and molecular markers. DNA was extracted from 189 individuals and 8 microsatellite markers were genotyped individually in these 21 populations. A total of 53 alleles were obtained in all populations, with an average of 6.62 alleles per locus. The expected and observed heterozygosity was 0.38 and 0.62 respectively. The average polymorphism index content of SSR markers was 0.61, ranging from 0.31 to 0.81. The unweighted pair group method with arithmetic mean dendrogram clustered all the populations into two groups, each for them subdivided into 3 sub-groups according to geographical origin. Morphological variation showed that the populations were not grouped according to their geographical origin. Therefore, patterns of differentiation of morphological traits did not coincide with molecular differentiation, indicating that morphological variation does not reflect genetic subdivision in studied faba bean populations. Analysis of molecular variance revealed high levels of genetic variation (83%) within population and provides a good base for designing genetic improvement programs. The result of Principal Component Analysis (PCA) revealed that three dimensional principal components (PC1, PC2 and PC3) contributed 40.56% of the total variability and accounted with values of 20.64, 11.22 and 8.70%, respectively. Cluster analysis based on PCA indicated three separate groups of populations. The genetic relationships found between the 21 populations samples were the same in both the PCA and STRUCTURE analysis which support the results observed. These data may serve as a foundation for the development of faba bean breeding programs.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Rebaa¹,

Abid²,

Aouida³

et al. 2017

Physiol Mol Biol Plants

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“…Despite some classifications regarding it as Faba vulgaris Moench., faba bean is considered by a vast majority a separate species within the genus Vicia L. (Suso et al 1993), with a number of chromosomes of 2n = 12 (Ouji et al 2011b). An analysis with ISSR markers with a large number of the local landraces of faba bean showed a great influence of their geographic origin and ecological factors, grouping those from Europe and North Africa on one side and those from Asia in another (Wang et al 2012).…”

Section: Faba Beanmentioning

confidence: 99%

Significance of genetic resources of cool season annual legumes: III: Locally cultivated and maintained crop landraces

Mikić¹,

Mihailović²

2014

Ratar i povrt

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Summary:The genetic resources of legumes (Fabaceae Lindl.) are facing the narrowing the genetic basis of cultivated species, that is bottlenecks of the available gene pools. This is caused mostly by breeding, since it aims at improving yield and quality. The most widely cultivated crops are in a specific danger of losing numerous desirable traits due to a heavy selection pressure preferring usually yield. Using locally cultivated and maintained landraces of the economically most important annual legumes, such as chickpea (Cicer arietinum), lentil (Lens culinaris), grass pea (Lathyrus sativus), white lupin (Lupinus albus) and faba bean (Vicia faba) may assist breeding and various modern crop systems. They preserved numerous desirable traits that may be easily introgressed into modern cultivars and thus improve their performance.

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“…Isozymes, the use of which was proposed by Hunter and Market (1957), have many advantages: they are direct products of the action of genes, they segregate according to Mendel's laws, and they usually are codominant. Besides studies on the genus Brassica (Yu et al, 2005), isozyme markers have been applied in a wide range of research on a variety of plant species (Weeden, 1989;Krzymińska et al, 2008;Ouji et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Markers employing PCR technique, such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), single sequence repeat (SSR) and single nucleotide polymorphism (SNP), support the selection of quantitative traits whose expression is considerably affected by environmental conditions (Javidfar et al, 2006;Hasan et al, 2008). They are used to construct genetic maps employed in many ways: as sources of genetic information describing species (Snowdon and Friedt, 2004;Chen et al, 2011), in research on the genetic diversity/similarity of breeding material Ouji et al, 2011;Zhao et al, 2012), and in constructing genet-ic pools for various breeding programs (Seyis et al, 2003;Hasan et al, 2005;Ofori et al, 2008;Wei et al, 2009;Krajewski et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Isozyme, RAPD and AFLP Markers in Genetic Similarity Assessment of CMS Ogura F1 Hybrids of Winter Oilseed Rape (Brassica Napus L.) Parental Lines

Liersch¹,

Bocianowski

Kozak³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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Isozyme, RAPD and AFLP markers were evaluated and compared for their ability to determine genetic similarity in a set of 18 parental lines of winter oilseed rape F 1 hybrids developed using CMS ogura. Five isozyme systems, 64 RAPD starters and 23 EcoRI+3/MseI+3 AFLP primer combinations generated 597 polymorphic markers. These polymorphic fragments were chosen for estimation of genetic similarity. Of the total number of polymorphic products, polymorphic zymograms constituted only 3.0% of the markers, 57 RAPD primers 37.7%, and 23 AFLP primer combinations 59.3%. The size of RAPD polymorphic products ranged from 564 to 2100 bp. On average there were four amplified bands per primer, with 61.0% polymorphism. The AFLP polymorphic fragments ranged from 72 to 1352 bp in size. AFLP assays generated 15 bands per primer pair on average and detected roughly four times more bands than with RAPD analysis. The genetic similarity coefficients based on all marker data range from 0.52 to 0.84. The correlation of genetic similarities based on RAPD and AFLP markers was 0.58. Estimated genetic similarity based on isozyme data was not correlated with genetic similarity derived from the two DNA-based markers. The dendrogram constructed with the three types of markers taken together grouped all the winter oilseed rape parental lines into several similar clusters. The genomic distribution and frequency of the RAPD and AFLP markers can serve well as estimators of genetic similarity between parental lines of F 1 CMS ogura hybrids.K Ke ey y w wo or rd ds s: : Winter oilseed rape, Brassica napus, F 1 hybrids CMS ogura, genetic similarity, molecular markers.

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Genetic diversity of nine faba bean (Vicia faba L.) populations revealed by isozyme markers

Cited by 16 publications

References 32 publications

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Significance of genetic resources of cool season annual legumes: III: Locally cultivated and maintained crop landraces

Comparison of Isozyme, RAPD and AFLP Markers in Genetic Similarity Assessment of CMS Ogura F1 Hybrids of Winter Oilseed Rape (Brassica Napus L.) Parental Lines

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