Genetic diversity of Neisseria gonorrhoeae multi-antigen sequence types in Russia and Europe

Shaskolskiy, Boris; Dementieva, Ekaterina; Kandinov, Ilya; Chestkov, A. V.; Kubanov, Alexey А.; Deryabin, D. G.; Gryadunov, Dmitry

doi:10.1016/j.ijid.2020.01.020

Cited by 16 publications

(19 citation statements)

References 19 publications

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“…A large number (27) of single ST isolates (i.e., STs represented by only one isolate) was found, a typical finding for the Russian Federation. The obtained data were similar to those for the NG-MAST types of isolates collected in the Russian Federation in 2013–2018, when STs 807, 228, and 1993 were also the most common STs [ 22 , 27 ].…”

Section: Resultssupporting

confidence: 78%

“…Genogroup G807, the most widespread genogroup in the Russian Federation, largely corresponds to the European genogroup G51 containing isolates obtained in the EU countries in 2013 [ 28 ], and the Russian genogroup G1152 corresponds to the European genogroup G387. In 2013–2018, the Russian genogroup G1152 was one of the most frequently isolated in Russia [ 22 ]. In the present work, isolates with STs 5734, 17,017, and 17,532 belonging to this genogroup were found; however, isolates of NG-MAST type 1152 were not found.…”

Section: Resultsmentioning

confidence: 99%

“…The Russian population of the causative agent of gonorrhea continues to demonstrate high resistance to penicillin, but no isolates resistant to ceftriaxone have been found to date [ 20 , 21 ]. This can be explained by the relative isolation of the Russian population of gonococci, as confirmed by the presence of sequence types (STs) and genogroups unique to the Russian Federation [ 22 ]. However, the evolution of drug resistance mechanisms and the transboundary transfer of globally widespread N. gonorrhoeae clones with established resistance mechanisms create a permanent risk of the emergence and spread of N. gonorrhoeae strains resistant to third-generation cephalosporins.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Typing of Neisseria gonorrhoeae Clinical Isolates in Russia, 2018–2019: A Link Between penA Alleles and NG-MAST Types

et al. 2020

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This work aimed to study penA gene polymorphisms in clinical isolates of Neisseria gonorrhoeae collected in Russia in 2018–2019 and the contribution of the penA allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates were analyzed. penA allele types were determined by sequencing, and the minimum inhibitory concentrations (MICs) of benzylpenicillin and ceftriaxone were measured. The influence of genetic factors on MICs was evaluated by regression analysis. All isolates were susceptible to ceftriaxone, and 40.1% of isolates were susceptible to penicillin. Eleven penA allele types were identified. The mosaic type XXXIV penA allele and the Gly120Lys substitution in PorB made the greatest contributions to increasing the ceftriaxone MIC; the presence of the blaTEM plasmid, Gly120Asp, Ala121Gly/Asn substitutions in PorB, and the adenine deletion in the promoter region of the mtrR gene caused an increase in the penicillin MIC. Among 61 NG-MAST types identified, the most frequent were types 228, 807, 9486, 1993, and 6226. A link between penA alleles and Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST) types was established. Resistance to two groups of β-lactam antibiotics was associated with non-identical changes in penA alleles. To prevent the emergence of ceftriaxone resistance in Russia, NG-MAST genotyping must be supplemented with penA allele analysis.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Typing of Neisseria gonorrhoeae Clinical Isolates in Russia, 2018–2019: A Link Between penA Alleles and NG-MAST Types

et al. 2020

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“…As stressed in the WHO global action plan [ 25 ] and the European response plan [ 26 ], it is essential to substantially enhance the quality assured surveillance of gonococcal AMR worldwide. Molecular epidemiological typing of gonococci can effectively support the gonococcal AMR surveillance and N. gonorrhoeae multiantigen sequence typing (NG-MAST) has been used in many countries globally [ 27 – 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…mostly countries earlier belonging to the Soviet Union). Accordingly, limited gonococcal AMR data have only been reported from Russia [ 33 , 38 – 41 ], Belarus [ 42 – 44 ], and Ukraine [ 45 , 46 ]. No AMR or phenotypic/genetic characteristics of N. gonorrhoeae strains spreading in Kyrgyzstan have been internationally published.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance and molecular epidemiological typing of Neisseria gonorrhoeae isolates from Kyrgyzstan in Central Asia, 2012 and 2017

et al. 2021

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Background Gonorrhoea and antimicrobial resistance (AMR) in Neisseria gonorrhoeae are significant public health concerns globally. Nearly no gonococcal AMR data are available from Central Asia, and no data from Kyrgyzstan has been published. We examined, for the first time, AMR and molecular epidemiology of N. gonorrhoeae isolates cultured in Kyrgyzstan in 2012 and 2017, in order to inform refinements of the Kyrgyz national gonorrhoea management guidelines. Methods N. gonorrhoeae isolates cultured in 2012 (n = 84) and 2017 (n = 72) in Kyrgyzstan were examined. MICs of nine antimicrobials were determined using Etest and, where available, clinical breakpoints from the EUCAST were applied. N. gonorrhoeae multiantigen sequence typing (NG-MAST) was also performed. Results The overall resistance levels were high to ciprofloxacin (88.5%), tetracycline (56.9%), benzylpenicillin (39.1%), and kanamycin (4.7%). Resistance to cefixime (0.6%, n = 1 isolate), azithromycin (0.6%, n = 1), and gentamicin (0.6%, n = 1) was rare. No resistance to ceftriaxone or spectinomycin was found. However, the proportion of isolates with decreased susceptibility (MIC = 0.125 mg/L) to ceftriaxone and cefixime was 12.8 and 11.5%, respectively. Gonococcal isolates were assigned 69 sequence types, of which 52 (75.4%) were new. Conclusions The gonococcal population in Kyrgyzstan in 2012 and 2017 showed a high genetic diversity. Ceftriaxone, 500–1000 mg, in combination with azithromycin 2 g or doxycycline, particularly when chlamydial infection has not been excluded, should be recommended as empiric first-line treatment. Spectinomycin 2 g could be an alternative treatment, and given with azithromycin 2 g if pharyngeal gonorrhoea has not been excluded. Fluoroquinolones, aminoglycosides, benzylpenicillin, or tetracyclines should not be used for empiric treatment of gonorrhoea in Kyrgyzstan. Timely updating and high compliance to national gonorrhoea treatment guidelines based on quality-assured AMR data is imperative. Expanded and improved gonococcal AMR surveillance in Kyrgyzstan is crucial.

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16S ribosomal assay for early diagnosis of gonococcal meningitis with negative CSF culture: A case report

Gao

Xia

Huang

et al. 2022

Journal of Infection and Chemotherapy

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Genetic diversity of Neisseria gonorrhoeae multi-antigen sequence types in Russia and Europe

Cited by 16 publications

References 19 publications

Molecular Typing of Neisseria gonorrhoeae Clinical Isolates in Russia, 2018–2019: A Link Between penA Alleles and NG-MAST Types

Molecular Typing of Neisseria gonorrhoeae Clinical Isolates in Russia, 2018–2019: A Link Between penA Alleles and NG-MAST Types

Antimicrobial resistance and molecular epidemiological typing of Neisseria gonorrhoeae isolates from Kyrgyzstan in Central Asia, 2012 and 2017

16S ribosomal assay for early diagnosis of gonococcal meningitis with negative CSF culture: A case report

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