Genetic diversity of mungbean (Vigna radiataL.) germplasm in Indonesia

Abstract

Despite widespread mungbean [Vigna radiata (L.) Wilczek] consumption in Indonesia, few molecular studies have been carried out on accessions and available data are minimal. In this study, we used 30 newly developed simple sequence repeat (SSR) markers designed from the mapped sequence scaffolds of the Korean Sunhwanokdu and Gyeonggijaerae 5 mungbean genomes. These markers were used to examine loci in 83 mungbean accessions collected from diverse geographical areas in Indonesia. A total of 107 alleles were detected among the accessions with 29 polymorphic markers. However, the mean of polymorphic information content (0.33) value and diversity index (0.38) value was indicative of low genetic diversity in this germplasm. The mungbean population structure was not clearly differentiated and the number of subpopulations was unclear. Neighbour-joining tree analysis revealed that the genetic cluster did not reflect the geographical origin of the accessions. Interestingly, the most agriculturally improved varieties were genetically similar to some landraces from one of the main mungbean-producing regions. These newly developed SSR markers could be useful for detecting genetic variability as a basis for establishing a conservation strategy for mungbean germplasm with the aim of enhancing Indonesian breeding programmes.

The Potential Use of SSR Markers to Support the Morphological Identification of Indonesian Mungbean Varieties

“…This study is in agreement with the previous study by Lestari et al (2014) and has also confirmed the usefulness of the SSR molecular marker set to identify mungbean varieties which had a narrow genetic background (Sestili et al 2011), suggesting to open new perspectives towards protection of released varieties and conservation of Indonesian mungbean genetic resources.…”

“…In this study, a total of 30 newly SSR simple sequence repeat (SSR) markers which were developed from the genome of Korean mungbean varieties (Sunhwanokdu and Gyeonggijaerae 5) proved their application to detect genetic variability of 83 accessions of Indonesian mungbean to support breeding program and conservation strategy. Moreover, these markers were able to identify improved mungbean varieties that were genetically similar to some landraces from one of the main mungbean-producing regions in Indonesia (Lestari et al 2014). Considering the prospecting SSR markers (Lestari et al 2014), our current study applied those markers in addition to other markers for varietal identification rather than genetic diversity study.…”

“…Moreover, these markers were able to identify improved mungbean varieties that were genetically similar to some landraces from one of the main mungbean-producing regions in Indonesia (Lestari et al 2014). Considering the prospecting SSR markers (Lestari et al 2014), our current study applied those markers in addition to other markers for varietal identification rather than genetic diversity study. The use of molecular markers which discriminate varieties in nucleotide sequences are unaffected by environments and becomes more desirable in varietal identification and differentiation.…”

Pra-pemuliaan Aneka Kacang dalam Mendukung Proses Pemuliaan untuk Perakitan Varietas Unggul Baru

“…Bank gen BB Biogen melengkapi koleksi internasional dengan sejumlah aksesi kedelai toleran kekeringan, tahan hama pengisap polong, tahan virus SSV, toleran naungan (Asadi, 2009;Asadi et al, 1997Asadi et al, , 2003Asadi et al, , 2012, dan toleran aluminium (Tasma et al, 2008), (Tabel 3). Beberapa aksesi lokal kacang hijau dari Nusa Tenggara diidentifikasi dalam kelompok yang mungkin telah beradaptasi pada lingkungan kering (Lestari et al, 2014a).…”

“…Marka SSR kacang hijau berbasis kedelai asal Korea telah berhasil membedakan varietas lokal kacang hijau asal Indonesia yang berasal dari daerah kering (Nusa Tenggara) (Lestari et al, 2014a). Marka molekuler telah dimanfaatkan untuk mengidentifikasi keragaman genetik plasma nutfah kedelai Indonesia (Chaerani et al, 2011;Santoso et al, 2006) dan terkait ketahanan terhadap keracunan aluminium (Tasma et al, 2009).…”