Genetic diversity of multidrug‐resistant <i>Mycobacterium tuberculosis</i> strains isolated from tuberculosis patients in Iran using MIRU‐VNTR technique

Khosravi, Azar Dokht; Shahraki, Abdolrazagh Hashemi; Dezfuli, Soolmaz Khandan; Hashemzadeh, Mohamad; Goodarzi, Hamed; Mohajeri, Parviz

doi:10.1016/j.kjms.2017.06.011

“…The results of this study showed that the genetic population structure of the included isolates was heterogeneous, like other low-incidence countries, and 99 different patterns were detected among the 100 isolates [ 4 ]. Likewise, these results were compatible with previous studies about the genetic population structure of M. tuberculosis in Iran [ [27] , [28] , [29] ].…”

Section: Discussionsupporting

confidence: 93%

Genotyping and drug susceptibility testing of Mycobacterium tuberculosis in Iran: a multi-centre study

Mousavi

¹

,

Amini

²

,

Mirsaeidi

³

et al. 2020

New Microbes and New Infections

View full text Add to dashboard Cite

Tuberculosis (TB) is a deadly infection and caused 1.4 million deaths in 2018. Assessing the geographic distribution of major lineages of Mycobacterium tuberculosis can contribute greatly to TB control. Mycobacterial interspersed repetitive unit variable number tandem repeat (MIRU-VNTR) typing is commonly used to differentiate various lineages of M. tuberculosis . A total of 2747 clinical specimens were collected consecutively from October 2018 through June 2019. Clinical isolates were identified as M. tuberculosis using standard biochemical tests. The standard 15-locus MIRU-VNTR typing was used for the genotyping of clinical isolates. Drug susceptibility testing was performed using the conventional proportion method. From the collected specimens, 100 were culture positive for M. tuberculosis . Using MIRU-VNTR, 99 different patterns were detected among the 100 isolates. They were distributed in one cluster comprising two strains and 98 unique patterns. Most of our isolates were similar to New-1 and Delhi/CAS strains. Of the M. tuberculosis isolates, 83 (83.0%) were pan-susceptible and 17 (17.0%) were resistant to at least one drug. Our study showed that MIRU-VNTR is a useful method for studying the genetic diversity of M. tuberculosis isolates in different regional settings and will help the health authorities to construct a preventive programme for TB.

show abstract

“…Furthermore, based on the allelic diversity, there is previous evidence suggesting that the number and combination of loci to be used to discriminate between genotypes can be adapted to the population of Mtb strains [46–48]. In this study, applying 12 loci MIRU [49, 50] allowed us to detect the existence of clusters with members of the LAM, H or S families respectively, with the presence of T isolates inserted in between. Interestingly, when evaluating conflicting genotypes, Ag85C SNP and Spotclust classification showed to be useful to solve the issues.…”

Section: Discussionmentioning

confidence: 94%

Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela

Acosta

¹

,

Russomando

²

,

Candia

³

et al. 2019

View full text Add to dashboard Cite

Background The Latin American & Mediterranean (LAM) spoligotype family is one of the most successful genotype of Mycobacterium tuberculosis worldwide and particularly prevalent in South-America. Within this family, a sublineage named Region of Difference Rio (RD Rio ) was reported initially in Brazil and is characterized by a genomic deletion of about 26.3 kb. This lineage seems to show a specific adaptation to the Euro-Latin American population. In this context, we sought to evaluate the LAM family and the presence of the RD Rio genotype in samples from three Latin American countries including Paraguay, Venezuela and Argentina. To detect LAM strains reliably we applied a typing scheme using spoligotyping, 12 loci MIRU-VNTR, the Ag85C 103 SNP and the regions of difference RD Rio and RD174. IS6110-RFLP results were also used when available. Results Genotyping of 413 M. tuberculosis isolates from three Latin-American countries detected LAM (46%) and the ill-defined T clade (16%) as the most frequent families. The highest clustering rate was detected in the sample population from the city of Caracas in Venezuela. We observed considerable differences in the presence of the RD Rio lineage, with high frequency in Caracas-Venezuela (55%) and low frequency in Buenos Aires-Argentina (11%) and Paraguay (10%). The molecular markers (RD174, Ag85C 103 , MIRU02-MIRU40 signature) of the RD Rio lineage were essentially confirmed. For the LAM family, the most polymorphic loci were MIRU40, MIRU31, MIRU10, MIRU26, MIRU16 and the least polymorphic MIRU24, MIRU20, MIRU04, MIRU23. Conclusions Our results suggest a differential adaptation of LAM-sublineages in neighboring populations and that RD Rio strains spread regionally with different rates of distribution. The Ag85C SNP and RDs (RD174, RD Rio ) tested in this study can in fact facilitate molecular epidemiological studies of LAM strains in endemic settings and low-income countries. Electronic supplementary material The online version of this article (10.1186/s12866-019-1479-6) contains supplementary material, which is available to authorized users.

show abstract

“…Most isolates were detected in sputum samples of patients with suspected pulmonary TB by direct microscopy and referred for further diagnostic procedures according to the indications mentioned in the national guideline for TB control [ 17 ]. Isolates were phenotypically identified as M.tb using acid-fast staining, culture on Löwenstein–Jensen (LJ) medium, and biochemical tests, including nitrate reductase, niacin accumulation, and catalase at 37 °C and 68 °C [ 18 ]. The M.tb H37Rv (ATCC® 27294) strain was used as the control strain.…”

Section: Methodsmentioning

confidence: 99%

Detection and characterization of mutations in genes related to isoniazid resistance in Mycobacterium tuberculosis clinical isolates from Iran

Bakhtiyariniya

¹

,

Khosravi

²

,

Hashemzadeh

³

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background The global rise in drug-resistant Mycobacterium tuberculosis ( M.tb ), and especially the significant prevalence of isoniazid (INH)-resistance constitute a significant challenge to global health. Therefore, the present study aimed to investigate mutations in prevalent gene loci—involved in INH-resistance phenotype—among M.tb clinical isolates from southwestern Iran. Methods Drug susceptibility testing (DST) was performed using the conventional proportional method on confirmed 6620 M.tb clinical isolates, and in total, 15 INH-resistant and 18 INH-susceptible isolates were included in the study. Fragments of six genetic loci most related to INH-resistance ( katG , inhA promoter, furA , kasA , ndh , oxyR-ahpC intergenic region) were PCR-amplified and sequenced. Mutations were explored by pairwise alignment with the M.tb H37Rv genome. Results The analysis of gene loci revealed 13 distinct mutations in INH-resistant isolates. 60% (n = 9) of the INH-resistant isolates had mutations in katG , with codon 315 predominately (53.3%, n = 8). Mutation at InhA − 15 was found in 20% (n = 3) of resistant isolates. 26.7% (n = 4) of the INH-resistant isolates had kasA mutations, of which G269S substitution was the most common (20%, n = 3). The percentage of mutations in furA , oxyR-ahpC and ndh was 6.7% (n = 1), 46.7% (n = 7), and 20% (n = 3), respectively. Of the mutations detected in ndh and oxyR-ahpC , 5 were also observed in INH-susceptible isolates. This study revealed seven novel mutations, four of which were exclusively in resistant isolates. Conclusions This study supports the usefulness of katG and inhA mutations as a predictive molecular marker for INH resistance. Co-detection of katG S315 and inhA -15 mutations identified 73.3% (11 out of 15 isolates) of INH-resistant isolates.

show abstract

Genetic diversity of multidrug‐resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU‐VNTR technique

Cited by 15 publications

References 35 publications

Genotyping and drug susceptibility testing of Mycobacterium tuberculosis in Iran: a multi-centre study

Genotyping and drug susceptibility testing of Mycobacterium tuberculosis in Iran: a multi-centre study

Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela

Detection and characterization of mutations in genes related to isoniazid resistance in Mycobacterium tuberculosis clinical isolates from Iran

Contact Info

Product

Resources

About