Genetic Diversity of Methylotrophic Bacteria from Human Mouth Based on Amplified Ribosomal DNA Restriction Analysis (ARDRA)

Waturangi, Diana Elizabeth; Francisca, Ivana; Susanto, Cindy Oktavia

doi:10.4308/hjb.18.2.77

Cited by 4 publications

(1 citation statement)

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“…The appearance of different ARDRA patterns of the same fungal species may be due to mutation in restriction site of Hae III and Hha I present in rRNA genes, and consequently showing changes in the RFLP pattern. A similar type of study conducted by Waturangi et al . grouped 21 bacterial isolates of different morphological characteristics from mouth microflora into nine OTUs based on ARDRA of 16S rRNA gene but DNA sequence analysis of six isolates of different OTUs identified them in the same species, i.e.…”

Section: Resultsmentioning

confidence: 91%

Investigation of diversity and dominance of fungal biota in stored wheat grains from governmental warehouses in West Bengal, India

2019

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BACKGROUND Fungal infestation is a leading cause of qualitative and quantitative deterioration of stored wheat grains. Limited information is available on the spatial distribution of fungal biota associated with stored wheat grains in India. Fungi were isolated and characterized from nine stored wheat grain samples in three warehouses of the Food Corporation of India, located in three agro‐climatic zones (Paschim Medinipur, Bankura and Purulia) of West Bengal in India. RESULTS Maximum density and fungal diversity were observed in dichloran glycerol agar (DG‐18) medium and the number increased with the increase of storage duration. Samples collected from Purulia showed maximum fungal diversity than that from Bankura and Paschim Medinipur. A total of 284 fungal isolates were obtained, classified into 29 operational taxonomic units (based on amplified ribosomal DNA restriction analysis of 18S and internal transcribed spacer sequences), and identified as 24 different fungal species. The majority of fungal isolates belonged to Aspergillus flavus (35%) followed by Rhizopus oryzae (13%) and Eurotium amstelodami (9%). Aspergillopepsin O (PEPO) gene and aflatoxin biosynthetic pathway gene, nor‐1, were amplified by polymerase chain reaction (PCR) from 91% and 71% of Aspergillus flavus isolates, respectively, indicating their aflatoxin producing ability. Aflatoxin production was further confirmed by ammonia vapour test, thin layer chromatography (TLC) and high‐performance liquid chromatography (HPLC). CONCLUSION The presence of toxigenic fungi in stored wheat grain emphasizes the necessity of quarantine measures of stored grains before placing them in the public domain to save consumers from health hazards. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 91%