Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers

Abstract

Genetic relationships among 40 loquat (Eriobotrya japonica (Thunb) Lindl) accessions that originated from different countries and that are part of the germplasm collection of the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were evaluated using microsatellites. Thirty primer pairs flanking microsatellites previously identified in Malus x domestica (Borkh.) were assayed. Thirteen of them amplified polymorphic products and unambiguously distinguished 34 genotypes from the 40 accessions analyzed. Six accessions showing identical marker patterns were Spanish local varieties thought to have been derived from 'Algerie' by a mutational process very common in loquat species. A total of 39 alleles were detected in the population studied, with a mean value of 2.4 alleles per locus. The expected and observed heterozygosities were 0.46 and 51% on average, respectively, leading to a negative value of the Wright's fixation index (-0.20). The values of these parameters indicate a smaller degree of genetic diversity in the set of loquat accessions analyzed than in other members of the Rosaceae family. Unweighted pair-group method (UPGMA) cluster analysis, based on Nei's genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. The high number of alleles and the high expected heterozygosity detected with SSR markers developed in Malus x domestica (Borkh.) make them a suitable tool for loquat cultivar identification, confirming microsatellite marker transportability among genera in the Rosaceae family.

Genetic relationships and origin of commercial clones of Nangouhi, a vegetatively propagated cultivar of hinoki cypress (Chamaecyparis obtusa)

“…Microsatellite genotypes of mutant sprouts are identical or very similar to each other. Previous studies failed to discriminate them or found only one locus that distinguished between the mutant sprouts and their original cultivars (Testolin et al 2000, Yamamoto et al 2003, Soriano et al 2005. In contrast, N6 and N13 had two and three distinguishable loci, respectively, of the ten loci.…”

“…In horticulture, mutant sprouts of specific cultivars are often used to produce new cultivars (Testolin et al 2000, Martins et al 2003, Yamamoto et al 2003, Hu et al 2005, Soriano et al 2005. Microsatellite genotypes of mutant sprouts are identical or very similar to each other.…”

Genetic Diversity and Utilization of Triploid Loquats (E. japonica Lindl)

“…Soriano et al (2005) assessed genetic relationships among 40 loquat accessions that originated from different countries, including accessions from the European loquat germplasm collection maintained at IVIA in Valencia, Spain. Thirty pairs of microsatellites previously identified in Malus × domestica (Borkh.)…”

“…2000; Cai 2000; Vilanova et al 2001;Soriano et al 2005;Dong 2008;Qiao 2008;Watanabe et al 2008;Yang 2009). These studies significantly enhanced our understanding about the distribution and structure of genetic diversity in loquat germplasm around the world.…”

Genetic Diversity of Walnut Revealed by AFLP and RAPD Markers

“…Moreover, the genetic diversity within or among species can also been analysed following these molecular marker techniques (Cervera et al, 2000;Qi et al, 2011). As a result of it, the development of molecular marker technique distinctly increases the study of plant genetic diversity (Soriano et al, 2005).…”