Genetic Diversity of Local Accessions of Dimocarpus longan Revealed By ISSR Markers

Abstract

ABSTRAK Keragaman Genetik Aksesi Lenggkeng Lokal ABSTRACTThere is no clear history of longan development in Indonesia even though it has developed well in Indonesia for many years.The study aimed at revealing genetic of diversity of local longan was done in Indonesian Citrus and Subtropical Research Institute (ICSFRI) from March to April 2008. ISSR markers were employed to identify the genetic variation among eight morphologically more or less alike accessions of longan ssp. Matrix data was counted and dendogram of samples was constructed using UPGMA and SAHN method. The cluster showed similarity value 0.34-0.86. The highest similarity value was observed between KL I and KL II (0.86) while the lowest one was obtained between KL I and KL V/KL VI (0.34). ISSR markers were able to identify the genetic diversity of longan and were helpful to provide information on genetic diversities especially for future breeding programs.

Genetic Variability Studies Based on Morphological and Physicochemical Characteristics Among Different Longan (Dimocarpus longan Lour.) Genotypes Under North Bihar Conditions

“…is one of the most important tropical and subtropical evergreen fruit crops that is native to Southern China and was introduced into India in 1798 (Yang et al, 2009;Huang et al, 2021). It is primarily subtropical necessitating a seasonal change in order to ower, but it is also planted in the tropics (Mariana et al, 2011;Puri, 2001;Yang et al, 2009). It is grown in over 20 countries, ranging from Sri Lanka and India to East Malaysia and Australia (Huang et al, 2021).…”

Development of SCAR makers for longan (Dimocarpus longan L.) authentication in Vietnam

“…Due to the high polymorphism level and the ability to discern between species, varieties and populations, molecular markers have been used as potential tools for the characterization of plants' gene pools (Oversná et al, 2002;Arif et al, 2010;Lammerts van Bueren et al, 2010). So far, several different molecular markers such 402 as RAPD (Random Amplified Polymorphic DNA), AFLP (Amplified Fragment Length Polymorphism), SCoT (Start Codon Targeted Polymorphism), ISSR (Intersimple sequence repeat) have been applied for longan genetic diversity assessment (Lynch and Milligan, 1994;Liu, 2001, Cheng et al, 2015;Mariana et al, 2011). These markers have played an important role to distinguish, characterize, and elucidate germplasm origin and diversity.…”

A First Phylogeny of the Genus Dimocarpus and Suggestions for Revision of Some Taxa Based on Molecular and Morphological Evidence

“…Given the agricultural and medicinal significance of longan, a number of studies have tried to develop molecular markers to assess the diversity of the numerous longan cultivars grown locally in China, Indonesia and Thailand as well as in germplasm collections from various regions. Such markers could potentially assist breeding program and facilitate authentication strategies such as, Random Amplified Polymorphic DNA (RAPD) 19 , Amplification Fragment Length Polymorphism (AFLP) 20 , Inter-Simple Sequence Repeat (ISSR) 21 and Single Nucleotide Polymorphism (SNP) 17 . Surprisingly however, no study has determined the genetic relationships between longan cultivars coupled with an evolutionary (phylogeny) analysis, or assessed the results in relationship to other taxa of the genus Dimocarpus .…”