Genetic diversity of Leishmania amazonensisstrains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping

Oliveira, João Paulo C de; Fernandes, Flora Maria de Campos; Cruz, Ângela K.; Trombela, Viviane A; Monteiro, Elisângela; Camargo, Anamaria A.; Barral, Aldina; Oliveira, Camila I. de

doi:10.1186/1475-9292-6-5

Cited by 37 publications

(38 citation statements)

References 46 publications

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“…In terms of geographical distribution, Dorval et al (2006), Marlow et al (2013) and Carvalho et al (2013) reported its detection in Mato Grosso do Sul, Santa Catarina and Rio de Janeiro states, Brazil. Regarding clinical manifestations, Barral et al (1991) and Oliveira et al (2007) (FREITAS et al, 1989;LAINSON & SHAW, 1968;BRASIL, 2013). These vectors are less anthropophilic, which explains a lower frequency of human infection with this Leishmania species.…”

Section: Discussionmentioning

confidence: 99%

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Caldart

Freire

Ferreira

et al. 2017

Rev. Bras. Parasitol. Vet.

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Leishmania in synanthropic rodents (Rattus rattus):new evidence for the urbanization of Leishmania (Leishmania) amazonensis AbstractThis study aimed to detect parasites from Leishmania genus, to determine the prevalence of anti-Leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of Londrina, Paraná, Brazil. Animal capture was carried out from May to December 2006, serological and molecular methods were performed. DNA was extracted from total blood, and nested-PCR, targeting SSu rRNA from Leishmania genus, was performed in triplicate. The positive samples were sequenced twice by Sanger method to species determination. In total, 181 rodents were captured, all were identified as Rattus rattus and none showed clinical alterations. Forty-one of the 176 (23.3%) animals were positive for Leishmania by ELISA and 6/181 (3.3%) were positive by IFAT. Nine of 127 tested animals (7.1%) were positive by PCR; seven were identified as L.

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Section: Discussionmentioning

confidence: 99%

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Caldart

Freire

Ferreira

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…The Leishmania isolate was obtained from a patient who presented diffuse form of CL in the state of Maranhão, Brazil. 19 The cells were maintained in alpha minimal essential medium (αMEM; Gibco ® ; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% inactivated fetal bovine serum (Multicell; Wisent Inc., St Bruno, QC, Canada), 100 μg/mL kanamycin (Sigma-Aldrich Co.), and 50 μg/mL ampicillin (SigmaAldrich Co.), and incubated at 24°C±1°C and pH 7.0 in BOD greenhouse (Model: 2005; Johns-VWR Scientific, Toronto, ON, Canada). The promastigotes were grown in cell culture flasks of 25 mL volume (Corning Incorporated, Corning, NY, USA) with an initial inoculum of 10 6 cells/mL and transferred to a new medium after reaching the stationary growth phase, twice a week.…”

Section: Animals and Parasitesmentioning

confidence: 99%

Polarity-sensitive nanocarrier for oral delivery of Sb(V) and treatment of cutaneous leishmaniasis

Lanza¹,

Corrêa-Júnior²,

Vilela³

et al. 2016

IJN

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There is a great need for orally active drugs for the treatment of the neglected tropical disease leishmaniasis. Amphiphilic Sb(V) complexes, such as 1:3 Sb– N -octanoyl- N -methylglucamide complex (SbL8), are promising drug candidates. It has been previously reported that SbL8 forms kinetically stabilized nanoassemblies in water and that this simple dispersion exhibits antileishmanial activity when given by oral route to a murine model of visceral leishmaniasis. The main objective of the present work was to interfere in the structural organization of these nanoassemblies so as to investigate their influence on the oral bioavailability of Sb, and ultimately, optimize an oral formulation of SbL8 for the treatment of cutaneous leishmaniasis. The structural organization of SbL8 nanoassemblies was manipulated through addition of propylene glycol (PG) to the aqueous dispersion of SbL8. The presence of 50% (v/v) PG resulted in the loss of hydrophobic microenvironment, as evidenced by fluorescence probing. However, nanostructures were still present, as demonstrated by dynamic light scattering, small-angle X-ray scattering, and atomic force microscopy (AFM). A remarkable property of these nanoassemblies, as revealed by AFM analysis, is the flexibility of their supramolecular organization, which showed changes as a function of the solvent and substrate polarities. The formulation of SbL8 in 1:1 water:PG given orally to mice promoted significantly higher and more sustained serum levels of Sb, when compared to SbL8 in water. The new formulation, when given as repeated doses (200 mg Sb/kg/day) to BALB/c mice infected with Leishmania amazonensis , was significantly more effective in reducing the lesion parasite burden, compared to SbL8 in water, and even, the conventional drug Glucantime ® given intraperitoneally at the same dose. In conclusion, this work introduces a new concept of polarity-sensitive nanocarrier that was successfully applied to optimize an oral formulation of Sb(V) for treating cutaneous leishmaniasis.

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“…Smear and culture were positive. The Leishmania identified as Leishmania (Viannia) braziliensis was performed by Polymerase Chain Reaction (PCR), RFLP and sequencing of ribosomal DNA from region ITS1 [6]. The HIV serology was negative and the patient was not taking any immunosuppressant drugs.…”

Section: Case Reportmentioning

confidence: 99%

Cutaneous Leishmaniasis Following Local Trauma: A Case Report

Sampaio¹

2014

CRDOA

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Genetic diversity of Leishmania amazonensisstrains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping

Cited by 37 publications

References 46 publications

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Polarity-sensitive nanocarrier for oral delivery of Sb(V) and treatment of cutaneous leishmaniasis

Cutaneous Leishmaniasis Following Local Trauma: A Case Report

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