Genetic Diversity of Korean Native Chicken Populations in DAD-IS Database Using 25 Microsatellite Markers

Abstract

A number of Korean native chicken(KNC) populations were registered in FAO (Food and Agriculture Organization) DAD-IS (Domestic Animal Diversity Information Systems, http://www.fao.org/dad-is). But there is a lack of scientific basis to prove that they are unique population of Korea. For this reason, this study was conducted to prove KNC's uniqueness using 25 Microsatellite markers. A total of 548 chickens from 11 KNC populations (KNG, KNB, KNR, KNW, KNY, KNO, HIC, HYD, HBC, JJC, LTC) and 7 introduced populations (ARA: Araucana, RRC and RRD: Rhode Island Red C and D, LGF and LGK: White Leghorn F and K, COS and COH: Cornish brown and Cornish black) were used. Allele size per locus was decided using GeneMapper Software (v 5.0). A total of 195 alleles were observed and the range was 3 to 14 per locus. The MNA, H exp , H obs , PIC value within population were the highest in KNY (4.60, 0.627, 0.648, 0.563 respectively) and the lowest in HYD (1.84, 0.297, 0.286, 0.236 respectively). The results of genetic uniformity analysis suggested 15 cluster (ΔK = 66.22). Excluding JJC, the others were grouped in certain cluster with high genetic uniformity. JJC was not grouped in certain cluster but grouped in cluster 2 (44.3%), cluster 3 (17.7%) and cluster8 (19.1%). As a results of this study, we can secure a scientific basis about KNC's uniqueness and these results can be use to basic data for the genetic evaluation and management of KNC breeds.(

Application of genomic big data to analyze the genetic diversity and population structure of Korean domestic chickens

“…Except for the NG and YO populations, all the chicken populations had a dominant single ancestor when K = 20. The ADMIXTURE analysis produced results similar to those of a diversity study using 25 MS markers; using 18 KDC populations, the groups were separated optimally at K = 15, and populations from the same ancestral species were classified together [13].…”

“…Several studies using MS markers have reported the genetic diversity of indigenous KNCs [9][10][11][12][13].…”

Genetic diversity and relationship of Korean chicken breeds using 12 microsatellite markers

“…Also, NIAS developed the Woorimatdag version 1 (WM1) and 2 (WMT). WM1 breeds were commercial, KNC breeds generated form crossbreeding fast growing native male and good tasting female with increased egg production, and WM2 breeds were modified version of WM1 breeds with increased growth rates (Park et al, 2010;Choi et al, 2015). The private native chicken breeding-stock company (Hanhyup) is responsible for more than 80% of the native chicken distribution in Korea and has maintained purebred chicken breeds (Hanhyup breeds) for commercial use for the past 60 years (Seo et al, 2018;Choi et al, 2019).…”