Genetic Diversity of Jatropha curcas in Southern Mexico

Ovando-Medina, Isidro; Adriano-Anaya, Lourdes; Vázquez-Ovando, Alfredo; Ruiz-González, Sonia; Rincón-Rabanales, Manuel; Salvador-Figueroa, Miguel

doi:10.1007/978-1-4614-4915-7_12

Cited by 15 publications

(18 citation statements)

References 81 publications

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“…However, there are still many technological challenges for its commercial establishment, including the lack of genetically improved varieties of high agronomic performance (Mishra, 2009;Ovando-Medina et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and structure ofJatropha curcasL. in its centre of origin

Salvador-Figueroa

Magaña-Ramos

Vázquez-Ovando

et al. 2014

Plant Genet. Resour.

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To investigate the genetic diversity and structure of Jatropha curcas L. oilseed plant, in this study, native populations from Chiapas, Mexico, were evaluated, using microsatellite DNA markers. A total of 93 representative samples were selected from seven sites in two regions in the state of Chiapas grouped by geographical proximity, where leaf samples were collected to isolate the genomic DNA. Individual polymerase chain reactions were carried out with ten pairs of specific oligonucleotides for the microsatellites of J. curcas, separating the products of amplification by acrylamide electrophoresis. Twenty-seven fragments were detected (77% polymorphic) with which heterozygous individuals were distinguished. The most informative microsatellite was Jcps20 (nine alleles, polymorphic index content 0.354). The average polymorphism per population was 58%. The Hardy -Weinberg tests revealed a reproductive pattern of non-random mating. The diversity descriptors and the analysis of molecular variance revealed that the populations were structured and moderately differentiated (F ST 0.087) and that this differentiation was not due to isolation by distance, as the Mantel test was not significant (P ¼ 0.137), but rather due to allopatry. Bayesian analysis revealed that the accessions belonged to only four genetic groups and confirmed the differentiation between the regions. Because some loci were in Hardy -Weinberg disequilibrium, it is proposed that differentiation is due to the clonal reproduction of J. curcas practised by farmers in Chiapas, along with the anthropogenic dispersion at regional levels. The results of this study reveal that J. curcas in Chiapas has genetic diversity that is greater than that reported in other parts of the world, which represents a potential germplasm pool for the selection of genotypes.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity and structure ofJatropha curcasL. in its centre of origin

Salvador-Figueroa

Magaña-Ramos

Vázquez-Ovando

et al. 2014

Plant Genet. Resour.

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“…Esto coincide con los estudios de Ovando-Medina et al (2013), quienes estudiaron la diversidad genética estimada con AFLPs, microsatélites, ácidos grasos de la semilla y caracteres de la flor, utilizando los mismos genotipos que en este trabajo, encontrando que J. curcas de la población Soconusco está diferenciada con respecto al resto de poblaciones. Es posible que en la región Soconusco los altos niveles de precipitación (hasta 4000 mm) hayan funcionado como factor de selección de los genotipos, los cuales se adaptaron diferenciándose, incluso reproductivamente de otras poblaciones.…”

Section: Discussionunclassified

“…De los sitios de origen de las accesiones estudiadas ninguno tiene más de 1500 mm de precipitación anual. En el trabajo de Ovando-Medina et al (2013) no se incluyeron accesiones de Veracruz, por lo que se requieren investigaciones adicionales con los genotipos de esa región.…”

Section: Discussionunclassified

Oleosomas de semillas de <em>Jatropha curcas</em> L. como estimadores de diversidad en poblaciones del Sur de México

Adriano-Anaya¹,

Gómez-Pérez²,

Ruiz-González³

et al. 2014

Grasas y Aceites

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SUMMARY:Seed oleosomes of Jatropha curcas L. as diversity estimators in populations of Southern Mexico. In this work, we used oleosome data to estimate the variability in eight J. curcas populations of Southern Mexico, using a multivariate approach. We found spherical oleosomes with diameters between 2.18 μm and 4.15 μm, which have two types of spatial arrangements: clustered and dispersed. A discriminant analysis showed that populations with more differentiation are Oaxaca and Veracruz, while Isthmus was the most diverse. The most informative variables for their contribution to the discriminant functions were oleosome size and number of oleosomes·μL of tissue −1. A heritability study with seeds harvested in two consecutive years showed that the variables oleosomes·μL of tissue −1 and oleosomes per cell have high broad sense heritabilities (h 2 bs =94.3% and h 2 bs =92.2%, respectively). A cluster analysis revealed high levels of dissimilarity between individuals. Correlation was found between the ratio oleosomes/tissue (volume/volume) and total oil content of the seed. The identification of genotypes with contrasting characteristics could provide the basis for future studies of the inheritance of characters in J. curcas. KEYWORDS: Diversity; Heritability; Jatropha; Oleosomes; Populations; Variability RESUMEN: Se estudiaron los caracteres de oleosomas de la planta para biocombustibles Jatropha curcas L. y se estimó la diversidad de ocho poblaciones del sur de México. Se encontraron oleosomas esféricos, con diámetros entre 2.18 μm y 4.15 μm, presentando dos tipos de arreglos espaciales: agrupados y dispersos. Un análisis discriminante mostró que las poblaciones más diferenciadas del resto son Oaxaca y Veracruz, mientras que Istmo fue la más diversa. Las variables más informativas por su contribución a las funciones discriminantes fueron tamaño de oleosoma y número de oleosomas·μL de tejido −1 . En un estudio de heredabilidad con semillas cosechadas en dos años consecutivos se encontró que las variables oleosomas·μL de tejido −1 y oleosomas por célula tienen muy altas heredabilidades en sentido amplio (h 2 bs =94.3% y h 2 bs =92.2%, respectivamente). Un análi-sis de conglomerados reveló altos niveles de disimilitud entre individuos. Los análisis de asociación de variables encontraron correlación entre la relación porcentual oleosomas/tejido (volumen/volumen) y el contenido total de aceite de la semilla.

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“…The genetic diversity of J. curcas in this Mesoamerican area and particularly in this region of Mexico is high [1,[4][5][6], and this may be largely due to the efficient pollination service by native stingless bees, to the strong trend for protogyny leading to pollination by xenogamy, and to the low prevalence of geitonogamy. Based in our results, strategies to improve the productivity of J. curcas in commercial plantations could be designed, for example increasing artificially the population of efficient pollinators as the stingless native bee S. mexicana.…”

Section: Native Stingless Bees S Mexicana T (T) Angustula T (Tmentioning

confidence: 99%

“…Jatropha curcas (L.) (Euphorbiaceae), possibly native to Mexico and Central America [1,2], is considered the most promising non-edible plant for the production of biofuels. Many countries have established programs for its commercial cultivation [3].…”

Section: Introductionmentioning

confidence: 99%