Genetic diversity of ITS sequences of Bursaphelenchus xylophilus

Cardoso, Joana M. S.; Fonseca, Luís; Abrantes, Isabel

doi:10.4238/2012.october.15.1

Cited by 13 publications

(8 citation statements)

References 32 publications

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“…However, some intraisolate genetic variability was found among the determined ITS sequences of BfungPt1 isolate, which is in agreement with the intraisolate molecular variability described for B. xylophilus (Cardoso et al 2012), Meloidogyne species (Hugall et al 1999) or non-phytoparasitic nematodes (Blouin 2002). However, some intraisolate genetic variability was found among the determined ITS sequences of BfungPt1 isolate, which is in agreement with the intraisolate molecular variability described for B. xylophilus (Cardoso et al 2012), Meloidogyne species (Hugall et al 1999) or non-phytoparasitic nematodes (Blouin 2002).…”

Section: Discussionsupporting

confidence: 85%

Bursaphelenchus fungivorus from Pinus pinaster bark in Portugal

et al. 2013

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Bursaphelenchus fungivorus is reported for the first time in Portugal, identified as associated with Pinus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphism analysis and sequencing of the internal transcribed spacer (ITS) regions of ribosomal DNA. Intraisolate genetic variability was detected among ITS sequences of the Portuguese B. fungivorus isolate. Phylogenetic analysis, obtained from multiple sequence alignment between ITS sequences of Bursaphelenchus species, revealed that the Portuguese B. fungivorus isolate clusters with other B. fungivorus isolates, forming a separate group close to B. seani highlighting a molecular proximity of these two species.

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Section: Discussionsupporting

confidence: 85%

Bursaphelenchus fungivorus from Pinus pinaster bark in Portugal

et al. 2013

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“…Cardoso et al . () observed that, in B. xylophilus , the ITS diversity was higher in the North American isolates (i.e. from the area of origin of this species) than in Japanese and Portuguese isolates (i.e.…”

Section: Discussionmentioning

confidence: 97%

Occurrence and ITS diversity of wood‐associatedBursaphelenchusnematodes in Scots pine forests in Switzerland

2015

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The quarantine pathogen Bursaphelenchus xylophilus (pine wood nematode, PWN) represents a serious threat for Pinus species in Europe. To exclude its presence in Switzerland, in 2010 and 2011 a countrywide survey was conducted in 102 Pinus sylvestris stands, chosen according to whether they contained dying or dead trees or were located in areas at risk of PWN introduction. In total, 285 trees (1–5 per site) were sampled. Nematodes were extracted from wood chips using a standard procedure, and identified to species by internal transcribed spacer (ITS) sequencing. Bursaphelenchus species were present in 34% of the trees, but no B. xylophilus was identified, i.e. PWN is still not present in Switzerland. The nematodes found belonged to seven different species, with B. vallesianus the most frequent species, followed by B. sexdentati, B. mucronatus kolymensis and B. eggersi. Three other species (B. borealis, B. pinophilus, B. poligraphi) were each only present in one or two trees. Three groups of sequences could not be assigned to a species because of the lack of matching reference sequences. The species composition found in Switzerland suggests co‐existence of southern and central European Bursaphelenchus species. Intraspecific ITS variability differed considerably among the four most common species. Bursaphelenchus eggersi, B. mucronatus kolymensis and B. sexdentati had several variable sites in the ITS region, resulting in multiple ITS genotypes in each species. In contrast, all 99 B. vallesianus isolates had an identical ITS region. This could indicate a founder effect, and possibly that B. vallesianus is not native to the Alpine region.

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“…Also, this region has shown high polymorphism between B. xylophilus and B. mucronatus populations, both inter-and intra-specific (Futai & Kanzaki, 2002;Cardoso et al, 2012). Likewise, De Luca et al (2011) reported high variability in populations of Pratylenchus spp.…”

Section: Discussionmentioning

confidence: 99%

“…Both regions are used since they provide a valuable tool in the identification of nematodes (PalomaresRius et al, 2010(PalomaresRius et al, , 2013 and reconstruction of phylogenetic relationships (Kaplan et al, 2000;Ye et al, 2007;Coomans et al, 2012). Although molecular studies for identification of B. xylophilus are abundant (Cao et al, 2005;Zhou et al, 2007;Jung et al, 2010;Cardoso et al, 2012;Valadas et al, 2013;Ye & Giblin-Davis, 2013), little is known about B. cocophilus. To date, only four sequences (SSU, LSU and mtCOI regions) of B. cocophilus collected in Costa Rica (one isolate) and Honduras (one isolate) from coconut and African oil palm are available in the NCBI database (Ye et al, 2007).…”

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confidence: 99%

“…In recent years, much work has been done on the molecular characterisation of different Bursaphelenchus species (Burgermeister et al, 2005(Burgermeister et al, , 2009Mota et al, 2006;Ye et al, 2007;Zhang et al, 2008;Li et al, 2009;Cardoso et al, 2012;Valadas et al, 2012Valadas et al, , 2013Marek et al, 2014). Within this genus, the LSU (D2-D3) and the ITS-1/2 ribosomal regions have been targeted for diversity studies (Mota et al, 2006;De Luca et al, 2011;Subbotin et al, 2011;Cardoso et al, 2012;Sultana et al, 2013) and for diagnosis purposes (Burgermeister et al, 2005(Burgermeister et al, , 2009. Both regions are used since they provide a valuable tool in the identification of nematodes (PalomaresRius et al, 2010(PalomaresRius et al, , 2013 and reconstruction of phylogenetic relationships (Kaplan et al, 2000;Ye et al, 2007;Coomans et al, 2012).…”

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confidence: 99%

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Genetic diversity of Bursaphelenchus cocophilus in South America

Silva

Parra

Campos

et al. 2016

Nematol

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Summary -Molecular characterisation of Bursaphelenchus cocophilus, the causal agent of 'red ring disease', is imperative for efficient identification procedures in Brazil and Colombia, because quarantine species such as B. xylophilus and B. mucronatus are already listed in both countries. ITS-1/2 region and D2-D3 segment of LSU rDNA were used to characterise isolates of B. cocophilus obtained from coconut plantations in Brazil and Colombia. Results from ITS-1/2 and LSU rDNA regions showed that all isolates of B. cocophilus from Brazil and Colombia formed a monophyletic group. The LSU rDNA region indicated that all isolates formed a single monophyletic group with high Bayesian posterior probability (100%). This is the first study on ITS-1/2 for the characterisation of B. cocophilus populations. A species-specific primer was designed for identification of B. cocophilus.

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Genetic diversity of ITS sequences of Bursaphelenchus xylophilus

Cited by 13 publications

References 32 publications

Bursaphelenchus fungivorus from Pinus pinaster bark in Portugal

Bursaphelenchus fungivorus from Pinus pinaster bark in Portugal

Occurrence and ITS diversity of wood‐associatedBursaphelenchusnematodes in Scots pine forests in Switzerland

Genetic diversity of Bursaphelenchus cocophilus in South America

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