Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems

Grange, Luciana; Hungría, Mariangela

doi:10.1016/j.soilbio.2004.03.005

Cited by 82 publications

(51 citation statements)

References 36 publications

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“…The strategy for assessing rhizobial diversity by isolating from root nodules collected directly from field was found to be better than the plants inoculated with soil dilutions (Loureiro et al 2007). The rhizobial diversity is depending on number of factors such as crop history and method of sampling (Grange and Hungria 2004). The rhizobial isolates in the current study were further tested on YEMA plates containing bromothymol blue which indicated that fast-growing isolates (18) were found to produce yellow colonies due to acid production on the medium with high mucous after 2 days of incubation (Table 3).…”

Section: Resultsmentioning

confidence: 91%

Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

Sharma¹,

Srivastava²,

Sharma³

2010

PLANT SOIL ENVIRON

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Soybean cultivation in many zones of India shows occurrence of native rhizobia besides other exotically adapted strains. In the current study, 22 rhizobial isolates (recovered from 12 different soybean growing sites) and 8 reference strains were selected for biochemical and metabolic characterization. Of 22 isolates, 18 were recovered as fast growing isolates while the rest were slow growing based on bromothymol blue (BTB) test. Unlike earlier belief that rhizobia have no ability to grow on glucose peptone agar medium, in this study, some isolates and some reference strains grew well on this medium. Similarly, when all the isolates were subjected to ketolactose test, some of the isolates were found to show growth on the medium. In contrast, based on C-utilization pattern (15 carbohydrates) a remarkable metabolic diversity was observed among the rhizobial isolates recovered in the study. The clustering and matching analysis showed that most of isolates were matching with slow growing reference strains, a few were with fast growing reference strains and some were found to be unique and hence not matching with any of reference strains. Such analysis suggests the occurrence of metabolically distinct types of rhizobia besides commonly known types (B. japonicum, B. elkanii and S. fredii) of soybean rhizobia and further validation is suggested through 16SrRNA gene sequencing technique.

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Section: Resultsmentioning

confidence: 91%

Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

Sharma¹,

Srivastava²,

Sharma³

2010

PLANT SOIL ENVIRON

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“…Collecting nodules directly from fields was considered the best method for assessing soybean rhizobial diversity, as in a recent study, more genotypes were trapped from field-grown soybean than from plants inoculated with soil dilutions under greenhouse conditions (Alberton et al 2006). However, it is noteworthy to comment that the choice of the sampling method varies with the host legume history, as for common bean (Phaseolus vulgaris L.), also in Brazil, higher diversity was obtained when nodules were sampled from plants inoculated with soil dilutions (Grange and Hungria 2004;Alberton et al 2006) andde Oliveira et al (2006) have demonstrated the feasibility of using direct PCR-denaturing gradients gel electrophoresis methods based on soil DNA.…”

Section: Resultsmentioning

confidence: 99%

“…In previous studies, we have shown that a level of similarity of 70% of the rep-PCR profiles analyzed in the Bionumerics program with a tolerance of 5% is adequate for the use in studies about rhizobial diversity in Brazilian soils (Grange and Hungria 2004;Alberton et al 2006;Kaschuk et al 2006a,b;Batista et al 2006); therefore, profiles showing similarity higher than 70% were also considered to be similar in this study.…”

Section: Genetic Diversitymentioning

confidence: 94%

Soybean [Glycine max (L.) Merrill] rhizobial diversity in Brazilian oxisols under various soil, cropping, and inoculation managements

et al. 2006

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In this study, soybean nodules were collected from 12 sites in the State of Mato Grosso, in the Brazilian Cerrados, where both exotic soybean [Glycine max (L.) Merrill] and bradyrhizobial strains have been introduced from 1 to 18 years before. All soils were originally devoid of rhizobia capable of effectively nodulating soybean and varied in terms of chemical and physical properties, inoculation procedures, and cropping systems. Rhizobial genetic diversity was assessed on 240 isolates by rep-PCR fingerprinting with BOX primer, and indices of diversity (abundance-based coverage estimator and traditional and modified Shannon indices) were applied to the profiles obtained. The genetic diversity was much greater than expected, as after the introduction of a maximum of four strains, up to 13 profiles were identified, some sharing many similar bands with the inoculant strains, but others quite distinct from the putative parental genotypes. The increase in the number of rep-PCR profiles could be attributed to genetic variability due to the stressful tropical environmental conditions, but also might indicate that indigenous rhizobia become capable of nodulating the host legume. After the third year of cropping with the host legume, inoculation did not affect rhizobial diversity. A high content of clay decreased diversity in comparison with that seen in a sandy soil, probably due to reduced aeration. Diversity was higher under the no-tillage system when compared to the conventional tillage management, highlighting the importance of maintaining crop residues in tropical soils. Understanding the ecology of exotic rhizobia after being introduced into new cropping areas represents a first step towards the establishment of better strategies of inoculation, which in turn may result in sustainability and higher plant yields.

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“…Indigenous bean rhizobia can be recovered from both uncropped and cropped soils in Brazil (7). In this study, none of the areas had a previous history of inoculation with rhizobia, thus the strains obtained represent the indigenous diversity.…”

mentioning

confidence: 82%

“…It is noteworthy that two isolates within group 2 [7,14] showed complete similarity of profiles although isolated from different sample locations. Groups 3 and 4 clustered only type and reference strains at 29% and 38% similarity, respectively.…”

mentioning

confidence: 99%

Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil

Cursino

Muro-Abad²,

Gomes³

et al. 2009

Braz. J. Microbiol.

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Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems

Cited by 82 publications

References 36 publications

Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

Soybean [Glycine max (L.) Merrill] rhizobial diversity in Brazilian oxisols under various soil, cropping, and inoculation managements

Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil

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