Genetic Diversity of<i>Pseudomonas solanacearum</i>: Detection of Restriction Fragment Length Polymorphisms with DNA Probes That Specify Virulence and the Hypersensitive Response

Cook, David E.; Barlow, E. Jed; Sequeira, Luís

doi:10.1094/mpmi-2-113

Cited by 180 publications

(146 citation statements)

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“…Clearly the species R. solanacearum comprises two divisions, which may represent subspecies as suggested by Li et al (1993). However, the ' Americanum ' and ' Asiaticum ' designations of these divisions proposed by Cook et al (1989) in relation to the presumed geographical origin of strains could be reconsidered since our analyses reveal an African biovar 1 subdivision, which may have its own centre of genetic diversity, and thus likely evolutionary origin, in Africa.…”

Section: Discussionmentioning

confidence: 92%

“…Thus, with 60 AFLP fingerprints (95 % of fragments were polymorphic), AFLP has a higher resolution level for intraspecific differentiation of R. solanacearum strains than PCR-RFLP (20 profiles for 178 strains tested) and RFLP (46 profiles for 164 strains tested) (Cook et al, 1989(Cook et al, , 1991Cook & Sequeira, 1994). Several DNA fragments were common to all R. solanacearum species.…”

Section: Clustering Of the Pcr-rflp And Aflp Profilesmentioning

confidence: 99%

“…R. solanacearum shows great phenotypic and genotypic diversity, usually being divided into five races based on host range (Buddenhagen et al, 1962 ;He et al, 1983 ;Pegg & Moffett, 1971) and six biovars based on biochemical properties (Hayward, 1964 ;Hayward et al, 1990 ;He et al, 1983). The use of RFLP (Cook et al, 1989 ;Cook & Sequeira, 1994) Abbreviations : AFLP, amplified fragment length polymorphism ; BDB, blood disease bacterium ; HCA, hierarchical cluster analysis ; UPGMA, unweighted pair group method with arithmetic averages.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

et al. 2000

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The genetic diversity among strains in a worldwide collection of Ralstonia solanacearum, causal agent of bacterial wilt, was assessed by using three different molecular methods. PCR-RFLP analysis of the hrp gene region was extended from previous studies to include additional strains and showed that five amplicons were produced not only with all R. solanacearum strains but also with strains of the closely related bacteria Pseudomonas syzygii and the blood disease bacterium (BDB). However, the three bacterial taxa could be discriminated by specific restriction profiles. The PCR-RFLP clustering, which agreed with the biovar classification and the geographical origin of strains, was confirmed by AFLP. Moreover, AFLP permitted very fine discrimination between different isolates and was able to differentiate strains that were not distinguishable by PCR-RFLP. AFLP and PCR-RFLP analyses confirmed the results of previous investigations which split the species into two divisions, but revealed a further subdivision. This observation was further supported by 16S rRNA sequence data, which grouped biovar 1 strains originating from the southern part of Africa.

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Section: Discussionmentioning

confidence: 92%

Section: Clustering Of the Pcr-rflp And Aflp Profilesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

et al. 2000

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“…To improve our understanding of the genetic relationship among the R. solanacearum species complex, DNA-based analyses were used, such as restriction fragment length polymorphisms (RFLP), polymerase chain reaction (PCR) -RFLP, 16S rRNA gene sequence analysis, repetitive extragenic palindromic (REP)-PCR, and amplified fragment length polymorphism (AFLP) (Cook et al 1989;Li et al 1993;Frey et al 1996;Poussier et al 2000b;Horita and Tsuchiya 2001). As examination of the diversity of the strains increased, it became clear that the R. solanacearum strains had four major phylotype subdivisions .…”

Section: Introductionmentioning

confidence: 99%

The sequevar distribution of Ralstonia solanacearum in tobacco-growing zones of China is structured by elevation

Liu

et al. 2016

Eur J Plant Pathol

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Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease resulting in tremendous losses of economic crops such as plants in the Solanaceae. Recent studies showed that R. solanacearum is spreading from the lowlands to the highlands in China. We studied 97 Chinese R. solanacearum strains that were isolated from four tobacco-growing zones over a wide range of elevations using phylotype specific multiplex polymerase chain reaction (Pmx-PCR) and phylogenetic relationships (egl and mutS). The results showed that all isolates belonged to phylotype I, which were further clustered into eight egl-sequence type groups (egl-group, sequevar): sequevars 13, 14, 15, 17, 34, 44, 54, and 55. In addition, Sequevar 55, found from the highlands, was a new/unknown one. Southeast China (Z3) had the largest number of egl-groups, containing six sequevars. The basin of the Yangzi River (Z1) and southwestern China (Z2) contained five egl-groups. The basin of the Huai River (Z4), near the north of China, where slight bacterial wilt occurred recently, contained a single group, sequevar 15. The distribution of sequevars was associated with elevation. Sequevar 15 was over-represented in lowland elevations, while sequevar 54 and the new/unknown one were only found in areas of moderate to high elevations. This finding suggested that the phylotype I strains infecting tobacco were diverse in China and regional integrated control strategies should be considered.

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“…Historically, R. solanacearum has been classified into five races and six biovars according to host range and trophic traits, respectively (Buddenhagen et al 1962;Hayward 1964;He et al 1983;Pegg and Moffett 1971). More recently, phylogenetic analysis described four distinctive phylotypes that are related to the geographical origin of the strains: phylotype I (Asia), phylotype II (America), phylotype III (Africa) and phylotype IV (Indonesia) (Cook et al 1989;Cook and Sequeira 1994;Fegan and Prior 2005). BW resistance is a key method for controlling the disease, together with agronomic practices such as crop rotation and fallowing.…”

Section: Introductionmentioning

confidence: 99%

Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

et al. 2012

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Resistance of eggplant against Ralstonia solanacearum phylotype I strains was assessed in a F 6 population of recombinant inbred lines (RILs) derived from a intra-specific cross between S. melongena MM738 (susceptible) and AG91-25 (resistant). Resistance traits were determined as disease score, percentage of wilted plants, and stem-based bacterial colonization index, as assessed in greenhouse experiments conducted in Réunion Island, France. The AG91-25 resistance was highly efficient toward strains CMR134, PSS366 and GMI1000, but only partial toward the highly virulent strain PSS4. The partial resistance found against PSS4 was overcome under high inoculation pressure, with heritability estimates from 0.28 to 0.53, depending on the traits and season. A genetic map was built with 119 AFLP, SSR and SRAP markers positioned on 18 linkage groups (LG), for a total length of 884 cM, and used for quantitative trait loci (QTL) analysis.

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Genetic Diversity ofPseudomonas solanacearum: Detection of Restriction Fragment Length Polymorphisms with DNA Probes That Specify Virulence and the Hypersensitive Response

Cited by 180 publications

References 0 publications

Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision The GenBank accession numbers for the sequences determined in this work are AF207891–AF207897.

The sequevar distribution of Ralstonia solanacearum in tobacco-growing zones of China is structured by elevation

Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

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