“…ءb to ءf, bands containing IS1405b, IS1405c, IS1405d, IS1405e, and IS1405f, respectively, which were eluted for inverted PCR or for cloning as described in Results. Lanes: 1, strain PS68; 2, PSS4; 3, PS31; 4, PS-AU17; 5, PS21; 6, PS-RO1; 7, PS-CTW20; 8, PS-L1; 9, PSS180; 10, PS-AU5; 11, PS-CTW9; 12, PS-CTW31; 13, PS-PER1; 14, PS-PDN3; 15 ml of bacterial suspension on the ethidium bromide-stained gel. Since only 2 l of bacterial suspension was used for PCR, this method was able to detect 20 cells of R. solanacearum.…”