Genetic Diversity of <i>Fusarium oxysporum</i> Isolates from Cucumber: Differentiation by Pathogenicity, Vegetative Compatibility, and RAPD Fingerprinting

Vakalounakis, D. J.; Fragkiadakis, Georgios A.

doi:10.1094/phyto.1999.89.2.161

Cited by 132 publications

(108 citation statements)

References 36 publications

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“…The most root fresh weight was recorded by "Mena" genotype (2.50 gm) followed by "Seina 2" cucumber genotype (2.20 gm), whereas the least root fresh weight was recorded by "Melita" followed by "Biet alpha" cucumber genotypes (1.06 and 1.17 gm, respectively). Similar results on cucumber varieties and genotypes were reported by many investigators (Vakalounakis, 1995;Dyakunchak and Ostroukh, 1998;Punga and Parker, 2000;Murakami et al, 2003;Buriev et al, 2004). …”

Section: Data Insupporting

confidence: 86%

“…Similar results were obtained on Fusarium oxysporum f.sp. cucumerinum on cucurbits Smith et al, 2001;Wang et al, 2001;Vakalounakis, et al, 2004;Ninet, et al, 2005). Williams, et al (1990) reported that random amplified polymorphic DNA markers can detect a large number of genetic polymorphism and when linked to major genes can be potentially useful in identifying traits.…”

Section: Hamatum (Th) 4= Isolate Of T Reesei (Tr) 5= Isolate Of Tmentioning

confidence: 99%

“…Welsh and McClelland (1990) reported that random amplified polymorphic DNA could provide simple and reproducible fingerprints of germplasm by employing single, arbitrary chosen primers. Vakalounakis and Fragkiadakis (1999) found that a total of 106 isolates of Fusarium oxysporum obtained from diseased cucumber plants showing typical root and stem rot or Fusarium wilt symptoms were characterized by pathogenicity, vegetative compatibility, and random amplified polymorphic DNA (RAPD). Vakalounakis, et al, (2004) found that thirty-four isolates of Fusarium oxysporum, obtained in China from cucumber plants showing either Fusarium wilt (F. oxysporum f.sp.…”

Section: Introductionmentioning

confidence: 99%

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Studies on Fusarium wilt Disease of Cucumber

Al-Tuwaijri¹

2015

J App Pharm Sci

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Eight isolates of Fusarium oxysporum f.sp. cucumerinum; the causal organism of Fusarium wilt of cucumber plants; were isolated from wilted cucumber plant samples collected from eight different localities in Egypt . These isolates were pathogenic to the susceptible cucumber cultivar "Biet alpha". Isolate 3 was the most virulent, followed by isolate 4 , while the least pathogenic was isolate 5. Potato Dextrose medium was the best medium for linear and amount of growth for all tested isolates, whereas rate of growth varied between the different tested isolates with the different tested media. Optimum temperature for the fungal linear growth was 27°C, followed by 28°C, while the best amount of growth was obtained at 25°C for all tested isolates. Cucumber genotypes were susceptible to Fusarium oxysporum f.sp. cucumerinum and differed significantly in their susceptibility. The most tolerant cucumber genotype was "Zeina" as well as it recorded the highest survival plants. DNA analysis detected 33 loci with an average 4.125 loci/isolate. The highest number of polymorphic loci was 6 loci in both Fus 1&4 isolates, while the lowest was 1 loci in Fus 6 only. UPGMA cluster analysis divide into two sub clusters, one of them included Fus 6 only and the second include the rest seven isolates with different similarity values. 100 & 614 bp were detected in both highly pathogenic isolates and it could be carrying the genetic information affecting pathogenicity of Fusarium to cucumber plants. Grouping of Trichoderma isolates in UPGMA cluster analysis and variation in DNA profiles among isolates was not related to their biocontrol ability. Biocontrol agents great affected Fusarium growth in dual cultures and reduced it under laboratory conditions. Biocontrol agents minimized the disease parameters in comparing with infested control treatments by the most aggressive isolate under greenhouse condition.

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Section: Data Insupporting

confidence: 86%

Section: Hamatum (Th) 4= Isolate Of T Reesei (Tr) 5= Isolate Of Tmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Studies on Fusarium wilt Disease of Cucumber

Al-Tuwaijri¹

2015

J App Pharm Sci

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“…The lower stem and taproot were longitudinally sectioned for examination of internal tissues (Kim et al, 2001). Symptom evaluation was made based on a rating scale of symptoms proposed by Vakalounakis and Fragkiadakis (1999). 0 = No symptoms; 1 = Light yellowing of leaves, light or moderate rot on taproot and secondary roots and crown rot; 2 = Moderate or severe yellowing of leaves with or without wilting, stunting, severe rot on taproot and secondary roots, crown rot with or without hypocotyls rot and vascular discoloration in the stem; 3 = Dead of seedlings.…”

Section: Pathogenicity Testmentioning

confidence: 99%

Characterization of Fusarium oxysporum isolates from tomato plants in Algeria

Mohammed¹,

Hamini-Kadar²,

Kihal³

et al. 2016

Afr. J. Microbiol. Res.

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Fusarium oxysporum is an ubiquitous soil-borne fungus, having a high genetic and ecological diversity with the potential to cause diseases of many crop species of economic interest. Indeed, some strains of F. oxysporum known as pathogens generate common diseases such as wilting, root and crown rot on host plants. Two formae speciales are confined to the tomato: F. oxysporum f.sp. lycopersici (FOL) causing Fusarium wilt, while F. oxysporum f.sp. radicis-lycopersici (FORL) causes Fusarium crown and root rot. The study include 27 strains isolated from the stems, crown and roots of infected tomato plants; to confirm the identity of the fungus, the isolates were identified using analysis based on morphological criteria and sequencing of the translation elongation factor 1-alpha (TEF) gene using ef1 and ef2 primers. Twenty three strains belonged to F. oxysporum, three strains to Fusarium solani, and one strain to Fusarium redolens. Tomato seedlings were tested to confirm the pathogenicity of the isolates tested. Pathogenicity test confirmed that twenty two F. oxysporum isolates were pathogenic on tomato and produced crown and root rot typical of F. oxysporum f.sp. radicis-lycopersici forma specialis, while one strain initially identified as F. oxysporum did not induce disease symptoms and is considered as non-pathogenic. Additionally, no symptoms of Fusarium wilt were observed at all; therefore no strains can be affiliated to F. oxysporum f.sp. lycopersici forma specialis.

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“…The random-amplifi ed polymorphic DNA (RAPD) method has been successfully used to identify strains [7][8][9], to characterize races [10] and to analyze virulence variability related to genetic polymorphisms [11][12][13][14][15] in phytopathogenic fungi. It has also been used in the study of inter-and intraspecifi c variability among populations from different [16][17][18] and from the same geographic regions [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Morphological and genetic differentiation among four pigment producing Indian species of Phoma (Saccardo, 1899)

Chande

Kövics

Sandhu³

et al. 2010

Indian J Microbiol

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A PCR-based technique, involving the random amplifi cation of polymorphic DNA (RAPD), was used for assessing genetic relatedness among isolates of the genus Phoma. Randomly Amplifi ed Polymorphic DNA (RAPD) revealed the presence of interspecifi c genetic variation among the pigment producing isolates of Phoma and has shown distinct phylogenetic cluster. The major objective of the study was to study the genetic variation, if any. Study was aimed to differentiate four pigment producing species of Phoma based on morphological studies and molecular markers in general and RAPD in particular. We found that the test species of Phoma can be very well differentiated using molecular markers. Phoma sorghina was differentiated from P. exigua, P. fi meti and P. herbarum. RAPD profi les of P. herbarum and P. fi meti has shown the maximum similarity, which indicates the genetic relatedness among these two species which were considered earlier as distinct species based on morphological observation.

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Genetic Diversity of Fusarium oxysporum Isolates from Cucumber: Differentiation by Pathogenicity, Vegetative Compatibility, and RAPD Fingerprinting

Cited by 132 publications

References 36 publications

Studies on Fusarium wilt Disease of Cucumber

Studies on Fusarium wilt Disease of Cucumber

Characterization of Fusarium oxysporum isolates from tomato plants in Algeria

Morphological and genetic differentiation among four pigment producing Indian species of Phoma (Saccardo, 1899)

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