Genetic diversity of Diaphorina citri and its endosymbionts across east and south‐east Asia

Abstract

The 'Ca. C. ruddii' is more diverse and structured than the D. citri and the 'Ca. P. armatura' across east and south-east Asia. Multiple introductions of the psyllid have occurred in China. Management application for controlling the pest is proposed based on the genetic information of D. citri and its endosymbionts. © 2017 Society of Chemical Industry.

Phylogeography of Diaphorina citri (Hemiptera: Liviidae) and its primary endosymbiont, ‘Candidatus Carsonella ruddii’: an evolutionary approach to host–endosymbiont interaction

“…Samples were preserved in 75% ethanol, then kept at 4 °C until DNA was extracted. One hundred and seventy specimens used in this study were also used in the study of Wang et al …”

“…To determine genetic diversity within C. ruddii , three single‐copy, housekeeping genes ( argH , dnaQ and rpoC ) were selected, and the PCR and sequencing procedures used are described in Wang et al . Genomic sequences of C. ruddii from the study of Wang et al . were also downloaded from GenBank.…”

“…Diaphorina citri is widely distributed in tropical and subtropical Asian countries, and is now also found in South, Central and North America, and East Africa . The biodiversity within D. citri from several regions has been identified through sequencing the mitochondrial COI gene, and using markers in the D. citri endosymbiont, Wolbachia wDi . Previous attempts to characterize biodiversity within accessions of D. citri across East and Southeast Asia used COI , but this marker does not explicitly incorporate geographic information .…”

“…The biodiversity within D. citri from several regions has been identified through sequencing the mitochondrial COI gene, and using markers in the D. citri endosymbiont, Wolbachia wDi . Previous attempts to characterize biodiversity within accessions of D. citri across East and Southeast Asia used COI , but this marker does not explicitly incorporate geographic information . However, its P‐endosymbiont shows more variation within populations across these regions .…”

“…Previous attempts to characterize biodiversity within accessions of D. citri across East and Southeast Asia used COI , but this marker does not explicitly incorporate geographic information . However, its P‐endosymbiont shows more variation within populations across these regions . Thus, molecular data from the P‐endosymbiont may provide a good opportunity to investigate the evolutionary pattern and history of the host–endosymbiont relationship.…”