Genetic Diversity of “
            <i>Candidatus</i>
            Liberibacter asiaticus” Based on Four Hypervariable Genomic Regions in China

Gao, Fanglan; Wu, Bo; Zou, Chunlin; Bao, Yixue; Li, Dean; Yao, Wei; Powell, Charles A.

doi:10.1128/spectrum.02622-22

Cited by 6 publications

(5 citation statements)

References 39 publications

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“…Furthermore, the reduced genetic diversity (Pi = 0.00005 ± 0.00001) observed within populations may elucidate the absence of a distinct geographic pattern. Low genetic diversity indicates comparatively minor genetic distinctions among individuals within the population, potentially attributable to genetic drift or other influencing factors [27,62].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a study utilized four hypervariable genomic regions to examine the genetic diversity of CLas in the Fujian and Guangxi provinces of China. Notably, the results indicated that the genetic diversity of CLas appeared to be unaffected by geographical location, citrus varieties, and HLB symptoms [27]. Nevertheless, these studies do not provide comprehensive information on the epidemiology and distribution of HLB in other regions of mainland China, and there is limited knowledge about the demographic history.…”

Section: Introductionmentioning

confidence: 94%

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Multi-Locus Sequence Analysis Reveals the Genetic Diversity and Population Differentiation of Candidatus Liberibacter Asiaticus in China

You,

Bao,

Amir

et al. 2023

Diversity

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Huanglongbing (HLB) is a devastating citrus disease caused by Candidatus Liberibacter asiaticus (CLas). Since its initial outbreak in Guangdong Province, China, it has spread to 10 provinces and caused significant economic losses. Hence, assessing CLas genetic diversity and demographic history is crucial for HLB epidemic prevention and control. In this study, we collected 500 leaf samples of CLas-infected plants from 10 provinces. We performed multi-loci sequence analysis on four gene fragments (omp, DnaA, GroEL, and SDE1) to explore the genetic differentiation and diversity of CLas in China. Our results indicated low nucleotide diversity (0.00005 ± 0.00001) in CLas, with the absence of significant systematic geographic structure in its distribution. Molecular variance analysis revealed predominant (81.7%) genetic variations within the population, with a minor variation (18.3%) occurring between populations as well as Yunnan provinces. In the Fujian population, significant gene exchange occurred with the other nine populations. Significant negative values in Tajima’s D and Fu’s FS neutrality tests indicated historical population expansions. The nucleotide mismatch distribution curve exhibits a single peak pattern, further supporting the expansion events. Our findings hold potential for advancing epidemiological research and providing suggestions for effective strategies to mitigate the spread of CLas and control HLB.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Multi-Locus Sequence Analysis Reveals the Genetic Diversity and Population Differentiation of Candidatus Liberibacter Asiaticus in China

You,

Bao,

Amir

et al. 2023

Diversity

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“…The outcomes indicate that the spectral response of trees affected by HLB was greater than that of unaffected trees in the nearinfrared red, and green spectral bands. Gao et al (2022), stated that the disease, commonly referred to as HLB has spread throughout areas that cultivate citrus fruits, including Guangxi, Guangdong, and Fujian. Fourie et al (2021) stated that HLB has yet to be detected in the southern African region.…”

Section: Figure 1hlb-infected Tree With Symptoms Of Blotchy Mottling ...mentioning

confidence: 99%

Decoding Huanglongbing: Understanding the Origins, Impacts, and Remedies for Citrus Greening

Usman,

Saleem,

Shafique

2023

Phytopathogenomics and Disease Control

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Citrus cultivation is a vital component of the agricultural sector, offering significant nutritional and economic benefits. However, citrus plants face numerous disorders, including Huanglongbing (HLB), also known as citrus greening disease. HLB is a devastating disease caused by the bacterial species Candidatus Liberibacter asiaticus (Las), primarily transmitted by the citrus psyllid and grafting. Investigating the distribution and molecular characterization of HLB in various citrus varieties and psyllid populations is crucial. Molecular techniques and iodine starch staining have been used to detect HLB symptoms. Several studies have been designed to determine the incidence and severity of HLB symptoms in different citrus varieties and locations and estimate the psyllid population density in different citrus varieties. Furthermore, these studies established a correlation between the population of psyllids and the incidence and severity of HLB in different locations and these findings suggest a potential link between psyllid infestation and the prevalence of HLB. This study highlights the prevalence of HLB in various locations and varieties, providing insights into the distribution, severity, and correlation between psyllid populations and HLB incidence. The reliability of PCR testing for Las infection confirms its effectiveness. Such findings suggest implementing integrated pest management strategies, including monitoring and controlling psyllid populations, removing infected plants, and using certified disease-free planting materials to prevent HLB spread.

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“…Here, we compare genomic diversity among six species of the Liberibacter genus using publicly available and assembled genome sequences. We are not the first to compare the genome content in Liberibacter species ( Thapa et al, 2020 ; Gao et al, 2022 ). For example, Thapa et al (2020) recently studied a sample of 37 Liberibacter genomes to investigate systematic relationships and genetic diversity within species.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of the Liberibacter genus reveals widespread diversity in genomic content and positive selection history

Batarseh

Morales‐Cruz

et al. 2023

Front. Microbiol.

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‘Candidatus Liberibacter’ is a group of bacterial species that are obligate intracellular plant pathogens and cause Huanglongbing disease of citrus trees and Zebra Chip in potatoes. Here, we examined the extent of intra- and interspecific genetic diversity across the genus using comparative genomics. Our approach examined a wide set of Liberibacter genome sequences including five pathogenic species and one species not known to cause disease. By performing comparative genomics analyses, we sought to understand the evolutionary history of this genus and to identify genes or genome regions that may affect pathogenicity. With a set of 52 genomes, we performed comparative genomics, measured genome rearrangement, and completed statistical tests of positive selection. We explored markers of genetic diversity across the genus, such as average nucleotide identity across the whole genome. These analyses revealed the highest intraspecific diversity amongst the ‘Ca. Liberibacter solanacearum’ species, which also has the largest plant host range. We identified sets of core and accessory genes across the genus and within each species and measured the ratio of nonsynonymous to synonymous mutations (dN/dS) across genes. We identified ten genes with evidence of a history of positive selection in the Liberibacter genus, including genes in the Tad complex, which have been previously implicated as being highly divergent in the ‘Ca. L. capsica’ species based on high values of dN.

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Genetic Diversity of “ Candidatus Liberibacter asiaticus” Based on Four Hypervariable Genomic Regions in China

Cited by 6 publications

References 39 publications

Multi-Locus Sequence Analysis Reveals the Genetic Diversity and Population Differentiation of Candidatus Liberibacter Asiaticus in China

Multi-Locus Sequence Analysis Reveals the Genetic Diversity and Population Differentiation of Candidatus Liberibacter Asiaticus in China

Decoding Huanglongbing: Understanding the Origins, Impacts, and Remedies for Citrus Greening

Comparative genomics of the Liberibacter genus reveals widespread diversity in genomic content and positive selection history

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