Genetic diversity of<i>Bradyrhizobium</i>strains isolated from<i>Arachis hypogaea</i>

Saleena, Lilly M.; Ponnusamy, Loganathan; Rangarajan, Sunita; Nair, Sudha

doi:10.1139/w00-139

Cited by 15 publications

(6 citation statements)

References 16 publications

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“…This distinction in relation to other soils in the growth pattern may be related to the pH 8.0 in the area, resulting in a more favorable environment for slow-growing rhizobium, under conditions of alkaline pH (Saleena et al, 2001). Acid reaction of pH in the culture medium was expressed by 28 % of the isolates from this soil (Figure 1-B1).…”

Section: Phenotypic Characteristics and Groupingmentioning

confidence: 89%

Biodiversity of rhizobia associated with cowpea cultivars in soils of the lower half of the São Francisco River Valley

Leite

Seido

Passos

et al. 2009

Rev. Bras. Ciênc. Solo

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The biodiversity of rhizobium in soils of the São Francisco Valley is unknown and can be studied using cowpea as trap plants. The objective of this study was to verify the diversity of diazotrophic bacteria that nodulate cowpea in soils of the lower half of the São Francisco River Valley by morphological and genotypic characterization. Seven soil samples (A1, A2, A3, A4, C1, C2 and MC) were collected to capture bacteria associated to five cowpea cultivars (IPA 206, BRS Pujante, BRS Marataoã, Canapu Roxo, and Sempre Verde), in a 5x7 factorial design with three replications. Thirty days after plant emergence, the nodules were collected and the bacteria isolated and analyzed in relation to their growth characteristics in YMA medium. The 581 isolates were grouped in 49 morphologic groups. Of this total, 62.3 % formed colonies in up to three days, 33.4 % grew from the 6 th day on, and 4.3 % began to grow 4 to 5 days after incubation. Regarding the formation of acids and alkalis, 63 % acidified the medium, 12 % made it alkaline and 25 % maintained the medium at neutral pH. The highest diversity was observed in the A3 sample and in isolates associated with the cultivars Canapu Roxo and BRS Pujante. Thirty-eight representative isolates were chosen for the genotypic characterization, clustered in four groups based on the restriction analysis of 16s rDNA. This grouping was strongly correlated with the sampling site; 13 rhizobium isolates had an electrophoretic profile distinct from the standard rhizobium strains used in this study.

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Section: Phenotypic Characteristics and Groupingmentioning

confidence: 89%

Biodiversity of rhizobia associated with cowpea cultivars in soils of the lower half of the São Francisco River Valley

Leite

Seido

Passos

et al. 2009

Rev. Bras. Ciênc. Solo

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“…7, group B). This might be due to variations in physical and chemical composition of the soil and variety of maize (host) from which they have been isolated (SALEENA et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Characterization and screening of bacteria from rhizosphere of maize grown in Indonesian and Pakistani soils

Naureen¹,

Yasmin²,

Hameed³

et al. 2005

J. Basic Microbiol.

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Thirty rhizobacteria isolated from maize grown in Pakistani and Indonesian soils were evaluated for their morphological characteristics, nitrogen fixation, P-solubilization, indole acetic acid (IAA) and siderophores production. Nitrogenase activity was detected in nineteen isolates ranging from 21.8-3624 n moles C2H4 produced/h/mg protein. Most of the isolates produced IAA, ten were capable of siderophore production while four were P-solubilizers. Ultrastructural studies of Pseudomonas sp. F14 indicated characteristic rhizospheric colonization within 48 h that was observed to change considerably with the passage of time from few bacteria to micro colonies. Random amplified polymorphic DNA (RAPD) analysis of 30 bacterial strains using 30 oligonucleotide primers resulted in considerable level of genetic diversity, with genetic distance ranging from 2-16%. Indonesian isolates were found to be more diverse as compared to Pakistani isolates. The characterization and screening of rhizobacteria of maize rhizosphere has helped in selection of isolates F7, LS-1, 3.1.1.C, F2, F3 and F13 as superior strains for use as bioinoculant. Moreover isolate F14 identified, as Pseudomonas fulgida by partial 16S rRNA sequence analysis is a novel strain regarding its tremendous potentials for inoculum production to enhance the yield of maize.

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“…Les applications de cette méthode en rhizobiologie concernent plutôt les bradyrhizobia (Nuntagij et al 1997;Saeki et al 2000;Saleena et al 2001;Sikora et al 2002) et des isolats d'Acacia tortilis subsp raddiana . Outre son intérêt taxonomique, cette technique est utile pour l'étude de la diversité infra-spécifique des bradyrhizobia (Lunge et al 1994;Nishi et al 1996).…”

Section: Rapd (Random Amplification Of Polymorphic Dna) Ou Ap-pcr (« unclassified

La taxonomie bactérienne moderne : revue des techniques — application à la caractérisation des bactéries nodulant les légumineuses (BNL)

Zakhia¹,

Lajudie²

2006

Can. J. Microbiol.

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Taxonomy is the science that studies the relationships between organisms. It comprises classification, nomenclature, and identification. Modern bacterial taxonomy is polyphasic. This means that it is based on several molecular techniques, each one retrieving the information at different cellular levels (proteins, fatty acids, DNA...). The obtained results are combined and analysed to reach a "consensus taxonomy" of a microorganism. Until 1970, a small number of classification techniques were available for microbiologists (mainly phenotypic characterization was performed: a legume species nodulation ability for a Rhizobium, for example). With the development of techniques based on polymerase chain reaction for characterization, the bacterial taxonomy has undergone great changes. In particular, the classification of the legume nodulating bacteria has been repeatedly modified over the last 20 years. We present here a review of the currently used molecular techniques in bacterial characterization, with examples of application of these techniques for the study of the legume nodulating bacteria.

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Genetic diversity ofBradyrhizobiumstrains isolated fromArachis hypogaea

Cited by 15 publications

References 16 publications

Biodiversity of rhizobia associated with cowpea cultivars in soils of the lower half of the São Francisco River Valley

Biodiversity of rhizobia associated with cowpea cultivars in soils of the lower half of the São Francisco River Valley

Characterization and screening of bacteria from rhizosphere of maize grown in Indonesian and Pakistani soils

La taxonomie bactérienne moderne : revue des techniques — application à la caractérisation des bactéries nodulant les légumineuses (BNL)

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