Genetic Diversity of Grasspea and Its Relative Species Revealed by SSR Markers

Fang, Wang; Yang, Tao; Burlyaeva, М. О.; Li, Ling; Jiang, Junye; Li, Fang; Redden, R.; Zong, Xuxiao

doi:10.1371/journal.pone.0118542

Cited by 34 publications

(48 citation statements)

References 28 publications

(34 reference statements)

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“…When a binary data matrix was generated, the original document was converted with Popgene 1.31 for genetic diversity parameter calculation, including the calculation of observed heterozygosity (H O ) of polymorphic markers, Shannon's information index (I), and the observed number of alleles (N A ; Shen et al, 2010;Xiao et al, 2014;Yu and Cheng, 2014). The NTsys2.1 software was used to construct the principal component analysis (PCA), which presents the distribution of populations (Cameron et al, 2015;Wang et al, 2015). The unweighted pair-group method with arithmetic averaging (UPGMA) dendrogram was constructed from the distance matrix imported from PowerMarker V3.25 using MEGA 4 (Sneath and Sokal, 1973;Nei and Takezaki, 1983;Tamura et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers

et al. 2015

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ABSTRACT. Genetic diversity and patterns of population structure of the 94 oil palm lines were investigated using species-specific simple sequence repeat (SSR) markers. We designed primers for 63 SSR loci based on their flanking sequences and conducted amplification in 94 oil palm DNA samples. The amplification result showed that a relatively high level of genetic diversity was observed between oil palm individuals according a set of 21 polymorphic microsatellite loci. The observed heterozygosity (H o ) was 0.3683 and 0.4035, with an average of 0.3859. The H o value was a reliable determinant of the discriminatory power of the SSR primer combinations. The principal component analysis and unweighted pair-group method with arithmetic averaging cluster analysis showed the 94 oil palm lines were grouped into one cluster. These results demonstrated that the oil palm in Hainan Province of China and the germplasm introduced from Malaysia may be from the same source. The SSR protocol was effective and reliable for assessing the genetic diversity of oil palm. Knowledge of the genetic 16248 L.X. Zhou et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 16247-16254 (2015) diversity and population structure will be crucial for establishing appropriate management stocks for this species.

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Section: Discussionmentioning

confidence: 99%

Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers

et al. 2015

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“…SSR is widespread throughout various eukaryotic genomes, and there are many reports [25][26][27] on the use of SSR to research species revolution and classification. As can be seen from dozens of species of animals and plants concerned, the SSR technique can quicken material classification and identification accurately.…”

Section: Discussionmentioning

confidence: 99%

Fingerprint analysis of Bombyx mori local variety resources based on SSR markers

Qian¹,

Li²,

Zhao³

et al. 2019

ScienceAsia

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41 Simple Sequence Repeats (SSR) markers were used to research the fingerprints of 96 silkworm local variety resources, including 83 Chinese univoltine varieties, 11 Chinese bivoltine varieties and 2 Japanese bivoltine varieties. These SSR markers showed rich polymorphism, with the number of alleles reaching 3-35, averaged 12.78, suggesting that there was rich SSR polymorphism among various local varieties and its polymorphism information content ranged from 0.162-0.958, averaged 0.690. The genetic distance between the various varieties was calculated from their fingerprints. By using UPGMA method, unique patterns of clustering among local species of silkworm were revealed. These included the patterns of 5 groups comprising of 54 varieties, while the other 42 species clustered in twos or threes, and as single nodes. In addition, the clustering among varieties did not indicate any obvious groups based on voltinism or moltinism, which seemed to support the theory of multiple origin of silkworm. The results prove that SSR markers can provide a fingerprint analysis of silkworm varietal resources and can be used for their classification and identification.

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“…Grass pea has a tremendous potential as a source of multiple stress-tolerant genes for general crop improvement with suitable breeding strategies (Hao et al 2017;Wang et al 2015). For example, donor germplasm with the desirable phenotypes can be used to form new breeding materials and available genomic tools can be applied to speed up the breeding process.…”

Section: Variety Amelioration Researchmentioning

confidence: 99%

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Lambein

Travella

Kuo

et al. 2019

Planta

124

113

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Main conclusion Although grass pea is an environmentally successful robust legume with major traits of interest for food and nutrition security, the genetic potential of this orphan crop has long been neglected. Grass pea (Lathyrus sativus L.) is a Neolithic plant that has survived millennia of cultivation and has spread over three continents. It is a robust legume crop that is considered one of the most resilient to climate changes and to be survival food during drought-triggered famines. The hardy penetrating root system allows the cultivation of grass pea in various soil types, including marginal ones. As an efficient nitrogen fixer, it meets its own nitrogen requirements and positively benefits subsequent crops. However, already in ancient India and Greece, overconsumption of the seeds and a crippling neurological disorder, later coined neurolathyrism, had been linked. Overemphasis of their suspected toxic properties has led to disregard the plant's exceptionally positive agronomic properties and dietary advantages. In normal socioeconomic and environmental situations, in which grass pea is part of a balanced diet, neurolathyrism is virtually non-existent. The etiology of neurolathyrism has been oversimplified and the deficiency in methionine in the diet has been overlooked. In view of the global climate change, this very adaptable and nutritious orphan crop deserves more attention. Grass pea can become a wonder crop if the double stigma on its reputation as a toxic plant and as food of the poor can be disregarded. Additionally, recent research has exposed the potential of grass pea as a health-promoting nutraceutical. Development of varieties with an improved balance in essential amino acids and diet may be relevant to enhance the nutritional value without jeopardizing the multiple stress tolerance of this promising crop.

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Genetic Diversity of Grasspea and Its Relative Species Revealed by SSR Markers

Cited by 34 publications

References 28 publications

Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers

Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers

Fingerprint analysis of Bombyx mori local variety resources based on SSR markers

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

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