Genetic diversity of Gracilaria changii (Gracilariaceae, Rhodophyta) from west coast, Peninsular Malaysia based on mitochondrial cox1 gene analysis

Yow, Yoon‐Yen; Lim, Phaik Eem; Phang, Siew Moi

doi:10.1007/s10811-010-9535-5

Cited by 29 publications

(17 citation statements)

References 59 publications

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“…Several studies assessing genetic diversity in the Gracilariaceae yielded intraspecific divergence; for example, 0.17 % in Gracilaria vermiculophylla (Yow et al 2011). Ten samples of H. edulis were genetically close (0-1.1 %), which were noticeably variable from the Japanese ones (2.0-2.6 %).…”

Section: Discussionmentioning

confidence: 99%

Molecular analyses for identification of the Gracilariaceae (Rhodophyta) from the Asia–Pacific region

Yang

Kim

2015

Genes Genom

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The family Gracilariaceae contains economically important seaweeds and is well known as a difficult group for the species identification due to its high species diversity and uncertainty of species limits. To date, molecular studies of the Gracilariaceae in the Asia-Pacific region have been insufficient to determine the distribution and diversity of species. To acquire a better understanding of the species boundary and species diversity, we analyzed mitochondrial COI as DNA barcoding marker and plastid rbcL genes for specimens from the Asia-Pacific region. A total of 22 species has been recognized as a distinct entity including five unidentified and two new distribution records. COI sequence data showed more variation than rbcL data, enabling the clear discrimination of species. The range of intra-and inter-specific divergence for COI was 0-2.6 % (except 4.4 %) and 2.8-15.7 %, respectively. RbcL data showed the interspecific divergences ranged from 1.1 to 14.8 % and divided into two large clades, such as Gracilaria sensu lato and Gracilariopsis. The regional study allowed us to get the information of the geographic distribution of two species, Gracilariopsis chiangii from Taiwan and Hydropuntia perplexa from Australia, and reported for the first time their presence in Okinawa, Japan. The database of the Gracilariaceae has potential to speed up the identification of species diversity.

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Section: Discussionmentioning

confidence: 99%

Molecular analyses for identification of the Gracilariaceae (Rhodophyta) from the Asia–Pacific region

Yang

Kim

2015

Genes Genom

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“…The PCR amplification was carried out in an Eppendorf Mastercycler ep gradient S thermocycler (Eppendorf, Hamburg, Germany) using the parameters as described by Yow et al (2011). All PCR products were electrophoresed through a 1% agarose gel stained with SYBR ® Safe DNA gel stain (Invitrogen, USA) to check the quality before being purified by LaboPass Gel and PCR Clean-up kit (Cosmo Genetech, South Korea).…”

Section: Methodsmentioning

confidence: 99%

Foliose Halymenia species (Halymeniaceae, Rhodophyta) from Southeast Asia, including a new species, Halymenia malaysiana sp. nov.

Tan¹,

Lim²,

Lin

et al. 2015

Botanica Marina

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Foliose Halymenia species (Halymeniaceae, Rhodophyta) from Southeast Asia, including a new species, Halymenia malaysiana sp. nov. Abstract: Despite the large number of species discovered in Halymenia, many remain poorly known due to the scarce information available. In order to facilitate species discrimination of foliose Halymenia species in Southeast Asia, molecular analysis and morphological studies were made on Halymenia collections from Malaysia, the Philippines, and Indonesia. The rbcL phylogenetic analyses showed that there are at least six taxa of foliose Halymenia occurring in Southeast Asia. Among the six taxa, a new species, Halymenia malaysiana P.-L. Tan, P.-E. Lim, S.-M. Lin et S.-M.Phang, is proposed based on both rbcL sequence analyses and morphological observations. Halymenia malaysiana is characterized by thalli possessing oblong or suborbiculate blades with a supple cartilaginous structure and gelatinous (slimy) texture, arising from a small discoid holdfast without a stipe, abruptly expanding into a broad blade and having a smooth surface with sinusoidally undulated margins. The phylogenetic analyses also revealed that Halymenia is a polyphyletic genus, which requires further taxonomic studies.

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“…Different molecular markers have been employed in studies of phylogeny and genetic diversity of Gracilariales; these include mitochondrial markers, like COI (Yang et al 2008;Yow et al 2011Yow et al , 2013 and the cox2-cox3 spacer (Rueness 2005;Destombe et al 2010;Pareek et al 2010;Garcı´a-Rodrı´guez et al 2013;Yang et al 2013); plastidencoded markers, like the RuBisCo spacer (Destombe & Douglas 1991;Byrne et al 2002;Iyer et al 2005;Guillemin et al 2008) and rbcL M.S. Kim et al 2006); and nuclear markers, like ITS (Goff et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Genetically recognizable but not morphologically: the cryptic nature ofHydropuntia corneaandH. usneoides(Gracilariales, Rhodophyta) in the Yucatan Peninsula

et al. 2015

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Species recognition in algae is often extremely difficult due to the paucity of morphological characters and high environmental plasticity. If environment is important to morphology, then related species growing in sympatry are good candidates to discover diagnostic characters. We studied algae collected in sympatric populations in tropical Atlantic Mexico. Hydropuntia cornea and H. usneoides have high morphological variation and have been difficult to diagnose morphologically. We used four molecular markers (RuBisCo spacer, cox2-3 spacer, rbcL and COI) to investigate the genetic relationship between samples that correspond to either H. cornea or H. usneoides; in addition, we determined if molecular-characterized groups were morphologically distinguishable. RuBisCo and cox2-3 spacers revealed low genetic variation but showed two genetic groups: groups I and II. rbcL and COI phylogenies also showed a separation into two groups, corresponding with cox2-3 spacer groups I and II. Group I matched sequences in GenBank of H. usneoides and group II with H. cornea. Populations were mixed for these genetic groups, with group I prominent in Quintana Roo populations. Morphological analysis of samples in both genetic clades showed that they are not distinguishable. As the difference between the two groups is only genetic, they are, consequently, cryptic species. While the extremes of morphology in allopatric populations may be distinguishable, we do not feel that in most cases these species can be recognized. Therefore, we propose that these two 'species' should be designated as a species complex (the Hydropuntia cornea/H. usneoides complex).

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Genetic diversity of Gracilaria changii (Gracilariaceae, Rhodophyta) from west coast, Peninsular Malaysia based on mitochondrial cox1 gene analysis

Cited by 29 publications

References 59 publications

Molecular analyses for identification of the Gracilariaceae (Rhodophyta) from the Asia–Pacific region

Molecular analyses for identification of the Gracilariaceae (Rhodophyta) from the Asia–Pacific region

Foliose Halymenia species (Halymeniaceae, Rhodophyta) from Southeast Asia, including a new species, Halymenia malaysiana sp. nov.

Genetically recognizable but not morphologically: the cryptic nature ofHydropuntia corneaandH. usneoides(Gracilariales, Rhodophyta) in the Yucatan Peninsula

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