Genetic diversity of flowering dogwood in the Great Smoky Mountains National Park

Hadziabdic, Ðenita; Wang, Xinwang; Wadl, Phillip A.; Rinehart, Timothy A.; Ownley, Bonnie H.; Trigiano, Robert N.

doi:10.1007/s11295-012-0471-1

Cited by 22 publications

(27 citation statements)

References 58 publications

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“…The helictid bees are only active locally (Rhoades et al, 2011). A microsatellite analysis of C. florida with more loci and dense sampling from the Great Smoky Mountains National Park (GSMNP) revealed local population structure and substantial north to south genetic discontinuity divided by the main ridges of the GSMNP (Hadziabdic et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Genetic structure and post‐glacial expansion ofCornus floridaL. (Cornaceae): integrative evidence from phylogeography, population demographic history, and species distribution modeling

Call

Sun

et al. 2015

J of Sytematics Evolution

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Repeated global climatic cooling and warming cycles during the Pleistocene played a major role in the distribution and evolution of the Earth biota. Here, we integrate phylogeography, coalescent-based Bayesian estimation of demographic history, and species distribution modeling (SDM) to understand the genetic patterns and biogeography of the flowering dogwood, Cornus florida subsp. florida L., since the Last Glacial Maximum (LGM). Natural populations of the species are severely threatened by dogwood anthracnose. We genotyped 306 plants from 73 locations of the species across most of its native distribution with three DNA regions from the plastid genome, ndhF-rpl32, rps16 and trnQ-rps16. The genealogy and haplotype network reconstruction revealed two haplotype lineages diverging %3.70 million years ago. We detected no clear geographic structuring of genetic variation, although significant local structure appeared to be evident, likely due to a combination of substantial localized seed dispersal by small mammals and small population size/limited sampling at a location. The spatial distribution of haplotype frequencies, estimated population demographic history, and results from hindcasting analysis using SDM suggested refugia in southeastern North America and population reduction during the LGM, followed by rapid post-glacial expansion to the north. Forecasting analysis using SDM predicted range shifts to the north under ongoing global warming. Our results further suggested that gene flow via seed dispersal has been high but insufficient to counter the effect of genetic drift. This study demonstrates the benefit of integrating genetic data and species distribution modeling to obtain corroborative evidence in elucidating recent biogeographic history and understanding of genetic patterns and species evolution.Key words: Bayesian skyride plot, Cornus florida, LGM, phylogeography, plastid DNA, post-glacial range expansion, species distribution modeling.Global climatic fluctuation in the Pleistocene had major impacts on genetic structure and distribution of plant species in North America (Comes & Kadereit, 1998;Hewitt, 2000). The Last Glacial Maximum (LGM) occurred roughly between 18 000-21 500 years ago and the Laurentide Ice Sheet extended to about 39°N at its southern-most extent (Ehlers & Gibbard, 2004). During this and additional glacial periods, thermophilic species retreated to the south or to local suitable microenvironments, but subsequently expanded distribution through post-glacial migration. According to the leading theory, these climatic oscillations led to the creation of refugia in which species survived during periods of colder temperatures (Hewitt, 2000(Hewitt, , 2001. Phylogeographic analyses have been widely used to uncover spatial patterns of genetic structure of various species and identify the possible refugia in which they persisted (e.g., Taberlet et al., 1998; Soltis et al., ). However, these analyses alone are inadequate for a complete understanding of the Pleistocene biogeography of ma...

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Section: Discussionmentioning

confidence: 99%

Genetic structure and post‐glacial expansion ofCornus floridaL. (Cornaceae): integrative evidence from phylogeography, population demographic history, and species distribution modeling

Call

Sun

et al. 2015

J of Sytematics Evolution

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“…The number of alleles/ locus was relatively low and ranged from 3 to 7 (mean 4.4), which has been observed in other endemic perennial species (Arroyo et al, 2016). The mean observed frequency of heterozygotes (H o ) in the total sample was 0.44, which deviated from the expected heterozygosity (H e ), indicating potential presence of population structure among the eight population collection areas (Hadziabdic et al, 2012). Expected heterozygosity ranged from 0.24 to 0.77, and all but one locus significantly deviated from Hardy-Weinberg equilibrium.…”

Section: Resultsmentioning

confidence: 80%

Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Amissah¹,

Wadl²,

Hadziabdic³

et al. 2016

J. Med. Plants Res.

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Cryptolepis sanguinolenta (Lindl.) Schlechter (Periplocaceae) is an herbaceous plant used in traditional medicine to treat malaria and populations of the species are diminishing due to overharvesting and lack of conservation. Codominant microsatellite markers that can be used to characterize genetic diversity and population structure are currently not available. Therefore, the study isolated 75 microsatellite loci from genomic sequence data, which were then screened for the ability to reveal polymorphisms. From the 75 candidate loci, 13 polymorphic microsatellite loci were optimized for future population genetics studies. Twenty-two C. sanguinolenta samples were collected from eight different geographical locations in Ghana. Alleles per locus ranged from 3 to 7 with a mean of 4.4. Expected heterozygosity ranged from 0.24 to 0.77, and all but one locus deviated significantly from Hardy-Weinberg equilibrium. Genetic differentiation mean was 0.06 among all loci, indicating relatively low genetic diversity in these samples. These microsatellite loci should be useful to study genetic diversity, gene flow and population structure as well as in a project involving breeding and conservation of C. sanguinolenta.

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“…One of the limitations of the QIAxcel system compared to other systems such as the ABI is the potential of not detecting heterozygotes of a 1 bp difference. When two flowering dogwood (C. florida) individuals were compared at 19 loci using the ABI or the QIAxcel system there was only a single individual with a 1 bp resolution difference on the ABI, thus confirming the utility of the QIAxcel system (Hadziabdic et al, 2012). The multilocus genotypic data for each individual was used to calculate the number of alleles per locus (A), number of effective alleles (N e ), expected (H E ) and observed heterozygosity (H O ) using GenAlEx 6.5 Smouse, 2006, 2012) for 9 C. mas and 2 C. florida microsatellite loci (Wang et al, , 2008Wadl et al, 2010).…”

Section: Discussionmentioning

confidence: 76%

“…The accessibly of reliable genetic markers is essential for variety identification and distinction, for development breeding method to create new cultivars, and to guarantee their proprietary protection. Various types of DNA markers such as DNA amplification fingerprinting (DAF) (Caetano-Anollés et al, 1999;Culpepper et al, 1991), microsatellites (Cabe and Liles, 2002;Hadziabdic et al, 2010Hadziabdic et al, , 2012Wadl et al, 2008aWadl et al, , 2008bWadl et al, , 2010Wadl et al, , 2012Wadl et al, , 2013Wang et al, 2008) and amplified fragment length polymorphism (AFLPs) (Mmbaga and Sauve, 2007;Smith et al, 2007) have been used to determine genetic diversity in Cornus species.…”

Section: Introductionmentioning

confidence: 99%

Development of microsatellites from Cornus mas L. (Cornaceae) and characterization of genetic diversity of cornelian cherries from China, central Europe, and the United States

Wadl

Szyp-Borowska

Piórecki

et al. 2014

Scientia Horticulturae

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Genetic diversity of flowering dogwood in the Great Smoky Mountains National Park

Cited by 22 publications

References 58 publications

Genetic structure and post‐glacial expansion ofCornus floridaL. (Cornaceae): integrative evidence from phylogeography, population demographic history, and species distribution modeling

Genetic structure and post‐glacial expansion ofCornus floridaL. (Cornaceae): integrative evidence from phylogeography, population demographic history, and species distribution modeling

Characterization of thirteen microsatellite loci from the Ghanian antimalarial plant Cryptolepis sanguinolenta

Development of microsatellites from Cornus mas L. (Cornaceae) and characterization of genetic diversity of cornelian cherries from China, central Europe, and the United States

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