Genetic Diversity of Five Lathyrus Species using RAPD, ISSR and SCoT Markers

Osman, Samira A.; Ali, Hoda Badry Mohammed

doi:10.3923/ajps.2020.152.165

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…On the other hand, the three types of DNA markers employed in this study, RAPD, ISSR, and SCoT, differ in the nature of the evolutionary mechanisms underlying their variation and their distribution in the genome (Osman and Ali 2020). According to our results, the detected average of polymorphism percent was 85%, 79.25%, and 69.44 for RAPD, ISSR, and SCoT markers, respectively, indicating the very high discriminating ability of the studied techniques and genotypes.…”

Section: Figure (4)mentioning

confidence: 61%

Assessment of Genetic Diversity in Summer Squash Genotypes Using some Yield Traits and DNA Markers Analysis under Sinai Conditions

El-Moneim¹,

Abdein²,

and³

2021

Journal of Plant Production Sciences

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Fourteen summer squash genotypes were used to assess their genetic diversity and relationships using some yield traits (Fruit length, fruit diameter, fruit shape index, average fruit weight, No of fruits/plant, fruit yield, and total soluble solids %) and DNA markers (Random Amplified Polymorphic DNA, inter-simple sequence repeat, Start Codon Targeted polymorphism) analyses. Two open field trials were executed at two locations, i.e., El-Arish (L 1 ) and Ras Sidr (L 2 ) in the North and South Sinai-Egypt, respectively. Significant differences were observed between both locations and indicated the superiority of (L 1 ) than (L 2 ) in all studied traits, while the interaction between genotype and location was significant for all studied traits. The location had a major effect on the relative genotypic potential of these traits, in which the genotype × location interaction was highly significant. SQ6 genotype ranked as the first in the fruit yield/plant and SQ2 for fruit diameter followed by SQ7 and SQ2 for fruit shape as well as SQ13 and SQ12 for the number of fruits/plant in L 1 and L 2 locations, respectively. Additionally, high polymorphism percentage (P %) was detected using RAPD (85%), ISSR (79.25%) and SCoT (69.44). Among the 15 studied primers, OP-B17 showed the maximum P% (100%) followed by primer OP-C17 (90.9 %) which also scored the highest values for polymorphism information content, effective multiplex ratio, and marker index. In addition, OP-K01 represented the maximum value of resolving power (12.56), indicated that these primers were highly informative. Intriguingly, 55 positive and two unique negative bands were amplified, out of which (33) bands were generated by RAPD followed by SCoT (15) and ISSR (9). In this regard, OP-C17 and SQ1 genotype produced the highest number of unique marker18 and 11amplicon, respectively. Moreover, the maximum similarity was found between SQ 11 and SQ 12 indicating that both genotypes were the most similar. Cluster and PCA analysis using RAPD, ISSR, and SCoT data grouped the 14 squash genotypes into four, three and two distinct clusters, respectively. Generally, the yield traits alongside molecular results showed significant information that may be utilized to sort and relationship between the studied summer squash genotypes under different conditions.

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Section: Figure (4)mentioning

confidence: 61%

Assessment of Genetic Diversity in Summer Squash Genotypes Using some Yield Traits and DNA Markers Analysis under Sinai Conditions

El-Moneim¹,

Abdein²,

and³

2021

Journal of Plant Production Sciences

View full text Add to dashboard Cite

show abstract

“…[21] revealed that the SCoT marker produced higher polymorphism (36.14%), PIC (0.142) and Rp (1.88) value as compared with the ISSR marker polymorphism (23-25%), PIC (0.088) and Rp value (1.55). On contrary, Osman and Ali [22] found slightly higher polymorphism (96.81%) and PIC (0.305) for the ISSR marker as compared with SCoT marker (polymorphism; 96% PIC;0.302) and RAPD marker (polymorphism; 94.2%, PIC;0.301). However, Collard and Mackill [23] suggested the use of SCoT markers being highly reproducible and more useful for targeted quantitative trait loci (QTL) mapping.…”

Section: Resultsmentioning

confidence: 84%

Gender-based Genetic Variability of Ailanthus excelsa Roxb, Populations Using, RAPD, ISSR and SCoT Markers

Shabnam

Ansari

Choudhary

et al. 2020

CJAST

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Ailanthus excelsa Roxb. is an economically important and multipurpose dioecious tree species of India, mainly used for fodder and timber. Gender-based genetic diversity of five populations of two sites (Jodhpur, Rajasthan and Deesa, Gujarat) of A. excelsa was assessed. A total of 42 RAPD, 20 ISSR and 23 SCoT primers were screened for DNA amplification of 232 individuals. Out of which only 25 primers (13 RAPD, 6 ISSR and 6 SCoT) were found polymorphic. The SCoT markers were showed the highest value for PIC, MI, Rp value, Nei’s gene diversity and Shannon’s index, as compared with the other two markers. Female individuals in all five populations had slightly higher genetic diversity as compared with male individuals. A high level of genetic diversity (55%) was detected within the populations of male and female individuals. High gene flow (6.70) and low genetic differentiation (0.069) values were found between Jodhpur and Deesa sites. Principal component analysis for all populations were accounted for 48.7% of the genetic variation. The Mantel test showed significant correlation (R = 0.178, P = .01) between genetic and geographic distances. The present study showed that SCoT markers were best for genetic diversity assessment in A. excelsa over RAPD and ISSR markers. High gene flow and low genetic differentiation in A. excelsa indicates its poor population fragmentation despite long geographic distances.

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“…Another explanation could be the low reproducibility of RAPDs [70]. In this respect, Osman and Ali [71] indicated that ISSR and SCoT markers are considered powerful markers for the discrimination and identification of five studied Lathyrus species. Moreover, in a study by Akçal-Giachino [72], the RAPD and ISSR marker systems were found to be useful for determining genetic diversity in P. anisum L. and identifying variations among landraces.…”

Section: Genetic Variations Revealed By Rapd and Issr Analysismentioning

confidence: 99%

Association between Productivity, Fatty Acid Profiles, Oil Bodies’ Ultrastructure and Molecular Markers in Peanut (Arachis hypogaea L.) Cultivars

et al. 2020

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The commercial Nigerian peanut cultivars Ex-Dakar, Samnut-22 and Samnut-26, the Egyptian cultivar Ismailia-1 and the American cultivar NC-7 were genetically differentiated and the cultivars’ effects on growth and yield components, total lipid contents, including saturated and unsaturated fatty acids, and oil body size and number were investigated. Experiments were carried out at Cairo University Research Station, Giza, Egypt, during the summer seasons of 2016 and 2017. All cultivars had high contents of total unsaturated fatty acids and low contents of saturated fatty acids. Variations among tested cultivars in oil body size and number were documented and cultivars were ranked based on their oil bodies’ size as Ismailia-1 > Samnut-26 > NC-7 > Ex-Dakar ≥ Samnut-22. Oil body size and number were inversely related. This variation can be explained by the different amounts of acidic amino acids and the protein structure on the surface of the oil bodies of these cultivars. The distributions of protein bodies and oil bodies seemed to be negatively correlated. Cultivar effect was significant, and variation was found in seed production and quality in addition to the size and number of oil bodies, oil productivity, and oil quality among cultivars. Seasonal effects were not evident due to the stability of the climatic conditions during the two seasons of the study.

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Genetic Diversity of Five Lathyrus Species using RAPD, ISSR and SCoT Markers

Cited by 6 publications

References 25 publications

Assessment of Genetic Diversity in Summer Squash Genotypes Using some Yield Traits and DNA Markers Analysis under Sinai Conditions

Assessment of Genetic Diversity in Summer Squash Genotypes Using some Yield Traits and DNA Markers Analysis under Sinai Conditions

Gender-based Genetic Variability of Ailanthus excelsa Roxb, Populations Using, RAPD, ISSR and SCoT Markers

Association between Productivity, Fatty Acid Profiles, Oil Bodies’ Ultrastructure and Molecular Markers in Peanut (Arachis hypogaea L.) Cultivars

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