Genetic diversity of Fasciola hepatica in Spain and Peru

Thang, Tran Nhat; Vázquez-Prieto, Severo; Vilas, Román; Paniagua, E.; Ubeira, F. M.; Ichikawa‐Seki, Madoka

doi:10.1016/j.parint.2020.102100

Cited by 15 publications

(8 citation statements)

References 25 publications

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“…Firstly, F. hepatica has not only normal spermatogenetic capability but also those of autofertilisation and cross-fertilisation. In some conditions, it can reproduce by parthenogenesis, and this particular mode of reproduction may cause changes in the allele frequencies in defined populations ( 10 , 26 , 27 ). Furthermore, the requirement of F. hepatica for intermediate hosts (mainly freshwater snails) and definitive hosts to complete its life cycle may also foster greater heterogeneity.…”

Section: Discussionmentioning

confidence: 99%

“…Marker molecules, such as the internal ribosomal transcribed spacers (ITS1 and ITS2), the phosphoenolpyruvate carboxykinase (pepck) gene, the DNA polymerase δ (pold) gene, and the 28S rRNA and 18S rRNA genes, have been used to identify F. hepatica, F. gigantica and their 'hybridisation' species (14,15,26). In addition, mitochondrial DNA with high mutation rate, simple molecular structure and rapid evolution (7,20), such as the NADH dehydrogenase subunit 1 (nad1) and the cytochrome c oxidase 1 (cox1) genes, have been widely used to analyse the genetic structure and dispersal pattern of F. hepatica populations (24,25,27).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China

Wang

Zhang

et al. 2022

Journal of Veterinary Research

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Introduction Fasciola hepatica is a trematode infecting ruminants worldwide and occasionally affecting other animal species, including humans. It causes significant economic losses. Geographic distribution and patterns of infection must be considered before control and management measures are developed for this parasite. DNA molecular markers are useful for the identification of flukes and elucidation of their genetic evolution. Therefore, the population structure of F. hepatica was studied using this method in sheep in Xinjiang, China. Material and Methods The molecular characteristics, genetic relationships within the population and dispersal patterns of F. hepatica isolates were analysed based on the cox1 and nad1 genes. The population structure of F. hepatica from three regions of Xinjiang was explored and a neutrality test was conducted. Results The cox1 and nad1 genes have 21 and 42 variable sites, respectively, which can be classified into 34 and 33 haplotypes. Median-joining network and phylogenetic tree analyses showed that there was no significant variation in F. hepatica isolates between the three geographical regions. Analysis of variance revealed that the genetic variation of F. hepatica was mainly present within the populations. The neutrality test indicated that the populations were relatively stable but the Hami population may have undergone short-term expansion. Conclusion This study revealed for the first time the molecular characteristics, genetic diversity and dispersal patterns of F. hepatica isolates from sheep in Xinjiang, thus providing new insights into the genetic variation and haplotype diversity of F. hepatica from indigenous sheep.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China

Wang

Zhang

et al. 2022

Journal of Veterinary Research

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“…Similar results in this species were observed when other variable markers were explored (e.g. mitochondrial or nuclear) in Spain (Thang et al ., 2020), Austria (Husch et al ., 2020), Armenia (Aghayan et al ., 2019), Iran (Bozorgomid et al ., 2020) and Australia (Elliott et al ., 2014). In the case of F. gigantica , high genetic diversity is also reported from Pakistan (Rehman et al ., 2020), Cambodia (Loeurng et al ., 2019) and Nigeria (Ichikawa-Seki et al ., 2017 b ) using mitochondrial markers.…”

Section: Biological Factors: a Glance At Highly Evolved And Adaptablementioning

confidence: 99%

Towards the comprehension of fasciolosis (re-)emergence: an integrative overview

2020

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The increasing distribution and prevalence of fasciolosis in both human and livestock are concerning. Here, we examine the various types of factors influencing fasciolosis transmission and burden and the interrelations that may exist between them. We present the arsenal of molecules, ‘adjusting’ capabilities and parasitic strategies of Fasciola to infect. Such features define the high adaptability of Fasciola species for parasitism that facilitate their transmission. We discuss current environmental perturbations (increase of livestock and land use, climate change, introduction of alien species and biodiversity loss) in relation to fasciolosis dynamics. As Fasciola infection is directly and ultimately linked to livestock management, living conditions and cultural habits, which are also changing under the pressure of globalization and climate change, the social component of transmission is also discussed. Lastly, we examine the implication of increasing scientific and political awareness in highlighting the current circulation of fasciolosis and boosting epidemiological surveys and novel diagnostic techniques. From a joint perspective, it becomes clear that factors weight differently at each place and moment, depending on the biological, environmental, social and political interrelating contexts. Therefore, the analyses of a disease as complex as fasciolosis should be as integrative as possible to dissect the realities featuring each epidemiological scenario. Such a comprehensive appraisal is presented in this review and constitutes its main asset to serve as a fresh integrative understanding of fasciolosis.

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“…Although discrimination errors in the fragment pattern analysis of the multiplex PCR for pepck have been reported in F. hepatica isolates from Afghanistan [8], Algeria [9], Ecuador [10], and Spain [11], subsequent nucleotide sequencing of DNA fragment of pepck enabled precise species identification. Regarding pold, discrimination errors were observed in F. gigantica isolates from Nigeria [12].…”

Section: Introductionmentioning

confidence: 99%

Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene

et al. 2022

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Background Multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) for nuclear phosphoenolpyruvate carboxykinase (pepck) and polymerase delta (pold), respectively, have been used to differentiate Fasciola hepatica, F. gigantica, and hybrid Fasciola flukes. However, discrimination errors have been reported in both methods. This study aimed to develop a multiplex PCR based on a novel nuclear marker, the fatty acid binding protein type I (FABP) type I gene. Methods Nucleotide sequence variations of FABP type I were analyzed using DNA samples of F. hepatica, F. gigantica, and hybrid Fasciola flukes obtained from 11 countries in Europe, Latin America, Africa, and Asia. A common forward primer for F. hepatica and F. gigantica and two specific reverse primers for F. hepatica and F. gigantica were designed for multiplex PCR. Results Specific fragments of F. hepatica (290 bp) and F. gigantica (190 bp) were successfully amplified using multiplex PCR. However, the hybrid flukes contained fragments of both species. The multiplex PCR for FABP type I could precisely discriminate the 1312 Fasciola samples used in this study. Notably, no discrimination errors were observed with this novel method. Conclusions Multiplex PCR for FABP type I can be used as a species discrimination marker in place of pepck and pold. The robustness of the species-specific primer should be continuously examined using a larger number of Fasciola flukes worldwide in the future since nucleotide substitutions in the primer regions may cause amplification errors. Graphical abstract

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Genetic diversity of Fasciola hepatica in Spain and Peru

Cited by 15 publications

References 25 publications

Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China

Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China

Towards the comprehension of fasciolosis (re-)emergence: an integrative overview

Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene

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