2013
DOI: 10.1016/j.rvsc.2012.07.011
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Genetic diversity of equine gammaherpesviruses (γ-EHV) and isolation of a syncytium forming EHV-2 strain from a horse in Iceland

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Cited by 20 publications
(25 citation statements)
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“…Alternatively, lack of amplification of the conventional PCR product may reflect the quality of template DNA, as qPCR was designed to amplify a much shorter product (~ 78 bp) than conventional PCR (> 1300 bp). Altogether, our results agree with the results of others [ 9 , 25 , 47 , 50 , 58 ], and confirm high genetic variability between field EHV-2 viruses. The importance of this variability on biological properties of the virus and interactions with its equine host remains to be elucidated.…”
Section: Discussionsupporting
confidence: 93%
“…Alternatively, lack of amplification of the conventional PCR product may reflect the quality of template DNA, as qPCR was designed to amplify a much shorter product (~ 78 bp) than conventional PCR (> 1300 bp). Altogether, our results agree with the results of others [ 9 , 25 , 47 , 50 , 58 ], and confirm high genetic variability between field EHV-2 viruses. The importance of this variability on biological properties of the virus and interactions with its equine host remains to be elucidated.…”
Section: Discussionsupporting
confidence: 93%
“…Moreover, EHV‐5 showed 95.1–99.5% nucleotide and 94.7–98.6% amino acid sequence identity with reference strains from GenBank. This high degree of genetic heterogeneity in equine gammaherpesviruses EHV‐2 and EHV‐5 is in agreement with previous studies conducted elsewhere (Bell et al., ,b; Ataseven et al., ; Brault et al., ; Thorsteinsdóttir et al., ). Whether this genetic heterogeneity of the gammaherpesviruses has an association with the clinical outcome or not is not clear and deserves further investigation.…”
Section: Discussionsupporting
confidence: 92%
“…Moreover, syncytia were observed upon nucleus staining following infection ( Fig. 1E to G ), a previously described feature of herpesvirus infection in vitro , including infection by equine gammaherpesvirus 2 ( 21 ). To confirm that the CPE was due to the BGHV8 identified by deep sequencing, we designed quantitative PCR (qPCR) primers based on the mRNA contigs from our RNA-seq data set (see Materials and Methods).…”
Section: Resultssupporting
confidence: 63%