Genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants

Velázquez, Encarna; Rojas, Marianela Chavarría; Lorite, María J.; Rivas, Raúl; Zurdo-Piñeiro, José Luis; Heydrich, Mayra; Bedmar, Eulogio J.

doi:10.1002/jobm.200700161

Cited by 64 publications

(30 citation statements)

References 36 publications

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“…Similar to the present study, Velázquez et al (2008) obtained endophytes from sugar cane that were phylogenetically very close to the following genera: Bacillus and Staphylococcus, from the Firmicutes group; Microbacterium, Micrococcus, and Kocuria from the Actinobacteria group; Rhizobium and Gluconacetobacter from the α-Proteobacteria group; Comamonas and Xanthomonas from the β-Proteobacteria group; and Acinetobacter and Pantoea from the γ-Proteobacteria group.…”

Section: Molecular Identification Of Isolates Based On Partial 16s Rdsupporting

confidence: 86%

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Rhoden

García

Silva³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide dispersion in South America, and phytochemical analyses from these plants and endophyte isolates have shown biological activity. Accordingly, the aim of this study was to verify the diversity of bacterial endophytes from T. elegans using partial sequencing of 16S rRNA, followed by phylogenetic analysis. Isolation was performed by cutting the leaves, after disinfection with 5% sodium hypochlorite (NaOCl), in 1-2-mm 2 fragments, which were equally placed on dishes containing TSA and fungicide BENLATE at 75 μg/mL. All dishes were incubated at 28°C in the biochemical oxygen demand system for 5 days and periodically checked. Afterwards, the colonization frequency (%) was determined: (number of fragments colonized by bacteria/total number of fragments) x 100. Three isolations between September 2011 and March 2012 were performed; the growth frequency ranged between 1.6 and 13.6%. Following sequencing of 16S rRNA and phylogenetic analysis, the genera identified were: Staphylococcus, Bacillus, Microbacterium, Pseudomonas, and Pantoea. These results will provide important knowledge on the diversity of endophytic bacteria inhabiting medicinal plants, and a better understanding of the microbiome of T. elegans would reinforce the necessity of endophyte studies with a focus on their future applications in biotechnological areas of agriculture, medicine, and the environment.

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Section: Molecular Identification Of Isolates Based On Partial 16s Rdsupporting

confidence: 86%

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Rhoden

García

Silva³

et al. 2015

Genet. Mol. Res.

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“…Stamford et al (1998) identified the genus Staphylococcus as endophytic of yam bean. A similar phenomenon was observed by Velásquez et al (2008), who were also able to identify the genus Staphylococcus as being endophytic of sugar cane through the analysis of 16S rRNA. Vendan et al (2010) also identified endophytic bacteria from ginseng and observed that the genus Staphylococcus, confirm the results obtained in this study.…”

Section: Characterization Of Classical Taxonomysupporting

confidence: 79%

Plant growth promoting potential of endophytic bacteria isolated from cashew leaves

Lins¹,

Fontes²,

Vasconcelos³

et al. 2014

Afr. J. Biotechnol.

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Endophytic microorganisms are able to promote plant growth through various mechanisms, such as production of plant hormones and antimicrobial substances, as well as to provide the soil with nutrients, for instance, inorganic phosphate. This study aimed to evaluate the potential of endophytic bacteria isolated from cashew leaves to produce substances involved in the promotion of plant growth, such as indole-3-acetic acid, the phosphate solubilization capacity, and the antimicrobial activity. For this, 31 isolate samples were used, out of which 17 (54.8%) produce indole-3-acetic acid in concentrations ranging from 11.79 to 145.85 µg.mL . It was observed that Fusarium oxysporum and Colletotrichum sp. were inhibited by 70 and 40% of the strains, respectively. It was found out that five bacteria (25%) were Gram-positive, predominantly the species Staphylococcus saprophyticus (100%), while 15 bacteria (75%) were Gram-negative. Out of these, 4 (26.6%) and 3 (20%) belong to the species Escherichia coli and Shigella flexneri, respectively. Studying the endophytic population is something important due its biotechnological applications, because it has a great potential for promoting plant growth.

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“…The nifH nucleotide sequences similar to those of Azonexus caeni and Rhodopseudomonas palustris were also similar to those of Zoogloea oryzae and Magnetospirillum magneticum, respectively. The genetic diversity of endophytic bacteria strains isolated from apoplastic sap of healthy sugarcane stems in Cuba showed a wide variety of phylogenetic groups (Velázquez et al 2008). Quantitative analysis of the diversity and population of actively N 2 -fixing endophytic bacteria should be advanced.…”

Section: Diversity Of Nifh Sequences By Dna Analysismentioning

confidence: 99%

Two seasons’ study on nifH gene expression and nitrogen fixation by diazotrophic endophytes in sugarcane (Saccharum spp. hybrids): expression of nifH genes similar to those of rhizobia

et al. 2010

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To explore the presence and expression of the nifH gene of diazotrophic endophytes in sugarcane (Saccharum spp. hybrids) cv. NiF8, we conducted pot experiments for two seasons in a glasshouse from 10 June to 17 September (high temperature and long days) in 2005 and 1 September to 9 December (low temperature and short days) in 2006. The expression of nifH genes in the stems and roots of sugarcane plants at 50 (or 59) and 100 days after transplanting was investigated by reverse transcription (RT)-PCR and by examining the nifH nucleotide sequence diversity. N 2 fixation was assessed by the 15 N-dilution method. The nifH RNA sequences in the stems and roots of the first experiment were similar to those of Bradyrhizobium sp. and Azorhizobium caulinodans. In the second experiment, nifH expression from these bacteria was not detected in the stems. nifH gene expression in the stems was in accordance with 18-24% N derived from air in the high-temperature season and negligible in the low-temperature season. Both the proliferation of N 2 fixers and expression of the nifH gene indicated that bacteria similar to rhizobia may contribute to N 2 fixation in sugarcane under our experimental conditions.

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Genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants

Cited by 64 publications

References 36 publications

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Plant growth promoting potential of endophytic bacteria isolated from cashew leaves

Two seasons’ study on nifH gene expression and nitrogen fixation by diazotrophic endophytes in sugarcane (Saccharum spp. hybrids): expression of nifH genes similar to those of rhizobia

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