Genetic diversity of eggplant (Solanum melongena) germplasm from Turkey assessed by SSR and RAPD markers

Abstract

ABSTRACT. Eggplant is a major crop in Turkey, which produces more of this crop than all of Europe; consequently, germplasm resources are of concern for the country. Molecular characterization of eggplant genotypes collected from different geographical regions of Turkey was carried out using SSR and RAPD markers. With amplification of five SSR loci, the number of alleles per microsatellite locus ranged from 2 to 10, with a total of 24 alleles. The greatest number of alleles was found at the emf21H22 locus (10 alleles); followed by emh11O01 and emf21C11 as five and four alleles, respectively. The average num ber of alleles per locus was 4.8. Using 11 decamer RAPD primers, 100 bands were amplified, among which 29 were polymorphic. The number of bands per primer ranged from seven (OPH10, OPH19, OPH20, OPH03) to 14 (OPB07). Primer OPB07 was the most polymorphic, generating 64% polymorphic bands; the rest of the primers gave less than 50% polymorphism. UPGMA dendrograms were used to examine the genetic relatedness of the genotypes.

Assessing diversity among traditional Greek and foreign eggplant cultivars using molecular markers and morphometrical descriptors

“…Morphological descriptors and molecular markers have been widely utilized for the characterization of eggplant material from different regions of origin, the study of diversity among different varieties and accessions and also the determination of the relationships among the cultivated eggplant with its weedy and wild forms and close relatives (Prohens et al, 2005;Isshiki et al, 2008;Demir et al, 2010;Ge et al, 2011Ge et al, , 2013Muñoz-Falcón et al, 2011;Tümbilen et al, 2011aTümbilen et al, , 2011bHurtado et al, 2012;Meyer et al, 2012;Vilanova et al, 2012;Cericola et al, 2013;Mutegi et al, 2015). Those studies have provided invaluable information regarding the management of germplasm collections, the conservation of 3 Diversity among traditional Greek and foreign eggplant cultivars…”

Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)

“…Also, many markers are available for each genome, there are high levels of polymorphism, and they are free of pleiotropic effects (Leal et al, 2010). During recent decades, SSRs (simple sequence repeats), also known as microsatellites, have become the most popular source of genetic markers owing to their high reproducibility, multi-allelic nature, codominant inheritance, high abundance, and wide genome coverage (Sharma et al, 2007;Demir et al, 2010;Cabral et al, 2011). The high infor mation content in SSR loci, together with their codominant expression, make SSRs ideal for genomic mapping, population genetics, conservation biology, marker-assisted selection, and other studies (Roa et al, 2000;Ellegren, 2004;Schlötterer, 2004;Leal et al, 2010).…”

Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA

“…In addition, traditional techniques are time-consuming and laborious. Therefore, molecular techniques have recently become a promising and versatile tool, especially for the detection of genetic variation (Demir et al, 2010;Abadio et al, 2012;Memati et al, 2012). Because these techniques are reliable, fast, sensitive, and suitable, they can replace traditional methods.…”