Genetic diversity of echovirus 30 involved in aseptic meningitis cases in Brazil (1998–2008)

Abstract

Aseptic meningitis is one of the most common neurological disorders caused by enteroviruses. Among them, Echovirus 30 (E30) is described as the main etiological agent of many outbreaks and sporadic cases. This study investigated the genomic variability of E30 isolated from the cerebrospinal fluid (CSF) of aseptic meningitis cases that occurred from 1998 to 2008 in Brazil. Over a 10-year period (1998-2008), 302 non-polio enteroviruses were isolated, of which 177 were identified as E30 (58.6%). Phylogenetic analysis of the complete VP1 gene (876 nt) of 48 E30 isolates was performed and compared with additional Brazilian and foreign strains. E30 VP1 sequences segregated into three distinct major groups and seven subgroups, which were linked to the isolation year. In general, sequence divergence among E30 strains ranged from 0.2% to 13.8%. A common direct ancestor for this set of E30 strains was not defined. Brazilian isolates from Group I were related genetically to a 1997 USA isolate and both may have a common origin. Group III representatives showed close relationship to the 2007 Argentinean isolates. The present results complement existing data on the molecular characterization and genetic variability of E30 and may contribute to the understanding of the epidemiology of aseptic meningitis in the region.

Detection and genotyping of enteroviruses in cerebrospinal fluid in patients in Victoria, Australia, 2007-2013

“…Their seasonal distribution followed the recognized pattern of peaks during summer with a reducing number of cases in subsequent months [Yamashita et al, ]. Members of HEV‐B have been widely described as the most common cause of aseptic meningitis cases and outbreaks worldwide [Bailly et al, ; Mirand et al, ; dos Santos et al, ]. However, this study also found a scattering of rarer types, often in single years.…”

Molecular identification of human enteroviruses associated with aseptic meningitis in Yunnan province, Southwest China

“…3) (Zhao et al 2005; dos Santos et al 2011). All Chinese strains belonged to group C, which further formed two distinct clusters.…”

Elucidation of Echovirus 30's Origin and Transmission during the 2012 Aseptic Meningitis Outbreak in Guangdong, China, through Continuing Environmental Surveillance

“…Numerous aseptic meningitis outbreaks that are caused by different lineages of ECHO30 have been reported during the last decade in many countries (15,21,32). In Guangdong Province, China, ECHO30 was first isolated from a sewage sample in 2010 through environmental surveillance; subsequently, this virus was continually but not frequently detected in sewage of Guangzhou City (7).…”

“…In the last few decades, repeated outbreaks and nationwide epidemics of ECHO30 have occurred in Europe (8)(9)(10)(11), Asia (12)(13)(14), and America (15)(16)(17). In China, high frequencies of ECHO30 detection in meningitis cases have been documented in the last few years in different coastal provinces, including Zhejiang (18), Jiangsu (14), and Shandong (19,20).…”