Genetic Diversity of Early Maturing Indian Maize (Zea mays L) Inbred Lines Revealed by SSR Markers

Analyses of genetic diversity among maize inbred lines differing for resistance to pink borer and post-flowering stalk rot

“…Various authors have suggested utility of SSR markers in diversity studies in maize (Vigouroux et al 2005;Legesse et al 2007;Kumar et al 2008;Qi-Lun et al 2008;Lia et al 2009;Shah et al 2009;Sharma et al 2010;Morales et al 2010). In the present investigation a total of 195 alleles were detected among the 23 lines with the 46 SSR primers used.…”

“…Equal quantity of leaves from 10 identical 25-30 days old seedlings of each genotype were bulked for DNA extraction following the method of Kumar et al (2008). A set of 46 SSR primer pairs (subsequently referred to as markers) distributed on both arms of all the maize chromosomes were obtained from Maize Database (www.agron.missouri.edu) ( Table 2).…”

“…A set of 46 SSR primer pairs (subsequently referred to as markers) distributed on both arms of all the maize chromosomes were obtained from Maize Database (www.agron.missouri.edu) ( Table 2). The PCR amplification, amplicon separation and scoring of band were carried out according to Kumar et al (2008).…”

“…However, because of their nonavailability in large number, environmental and inter-genic interactions, largely unknown control of such traits and inadequate sampling of the genome, such analysis does not reliably portray the true genetic relationship among the genotypes (Kumar et al 2008). From 1980s onwards DNAbased markers have successfully been employed to characterize germplasm in a large number of crop species including maize (Laborda et al 2005;Reif et al 2006;Legesse et al 2007;Kumar et al 2008;Qi-Lun et al 2008;Lia et al 2009;Shah et al 2009;Sharma et al 2010;Morales et al 2010). SSR or microsatellites are a group of DNA markers trusted widely for large-scale DNA fingerprinting, diversity analysis, gene mapping and QTL analysis in almost all crop species.…”

Genetic Diversity of Maize (Zea mays L.) Inbred Lines Revealed by Simple Sequence Repeat Markers

“…The average number of alleles (2.22), we observed in this study was considerably lower than those reported in maize by different researchers. Kumar et al, (2008) observed 2.96 in 16 popular inbred lines using 24 SSR markers. Warburton et al, (2002) found 4.9 alleles with 85 SSR markers.…”