Genetic diversity of Danthonia spicata (L.) Beauv. based on genomic simple sequence repeat markers

Thammina, Chandra; Amundsen, Keenan; Bushman, Shaun; Kramer, Matthew; Warnke, Scott E.

doi:10.1007/s10722-017-0596-z

Cited by 5 publications

(12 citation statements)

References 30 publications

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“…Both ITS groups were found at each collection site except the Liberty Reservoir Dam location, which was limited to three isolates from a small collection area. This random dispersal of the two ITS types is very similar to the diversity data that was observed with SSR markers in the host poverty oat grass (Thammina et al., 2018). The poverty oat grass data identified two groups with members of each group dispersed within collection locations and multilocus genotypes were identified from geographically separated population with diversity as a result of isolated mutations not distributed within collection locations.…”

Section: Discussionsupporting

confidence: 85%

“…The poverty oat grass data identified two groups with members of each group dispersed within collection locations and multilocus genotypes were identified from geographically separated population with diversity as a result of isolated mutations not distributed within collection locations. The poverty oat grass germplasm included in Thammina et al., 2018 was collected from 23 locations over a 402‐km range from Washington D.C. to North Carolina; however, very few of the collected plants exhibited choke. Poverty oat grass's unusual reproductive characteristics of small undeveloped anthers, nonfunctional lodicules, and no evidence for cross‐fertilization might suggest that its reproductive biology is being influenced in some way by A. hypoxylon .…”

Section: Discussionmentioning

confidence: 99%

“…The formation of the fungal choke generally occurs at the lowest node of the tiller that produces a nodal seed head. In the literature, the two seed heads are often referred to as chasmogamous (terminal) and cleistogamous (nodal) seed heads (Clay, 1982); however, this terminology is misleading, as poverty oat grass terminal flowers very rarely open and do not typically form viable anthers or pollen, making both seed heads functionally cleistogamous (Phillipson, 1986;Thammina, Amundsen, Bushman, Kramer, & Warnke, 2018;Weatherwax, 1928). Leuchtmann and Clay (1989) studied isozyme variation of 291 A. hypoxylon isolates from 24 populations of the four known host grasses flat-stem oat grass, downy oat grass, poverty oat grass, and Stipa leucotricha Trin.…”

Section: Crop Sciencementioning

confidence: 99%

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Genetic variation of the fungus Atkinsonella hypoxylon infecting poverty oat grass

2020

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Poverty oat grass [Danthonia spicata (L.) P. Beauv. ex Roem. & Schult.] is a perennial bunch‐type grass native to North America. Poverty oat grass is often present in managed turfgrass areas of the United States where cool‐season grasses are adapted and has potential for development as a seeded low‐input turfgrass option. Naturally occurring poverty oat grass plants occasionally exhibit choke on the flowering stems because of a proliferation of fungal hyphae by the ascomycete Atkinsonella hypoxylon (Peck) Diehl. (family Clavicipitaceae). Twenty‐five A. hypoxylon isolates were cultured from choke‐exhibiting poverty oat grass plants collected from five different populations. DNA variation was evaluated at the 5.8S ribosomal ribonucleic acid (rRNA) internal transcribed spacer (ITS) first (ITS‐1) and second (ITS‐2) regions and 10 simple sequence repeat (SSR) loci. Five single nucleotide polymorphisms (SNPs) in the ITS‐1 region and an addition–deletion in the ITS‐2 region identified two isolate groups present in four of the five populations. The 10 SSR loci exhibited 28 scorable alleles and support the two groupings with variation appearing as random mutations and high linkage disequilibrium providing no evidence for sexual recombination within or between the two identified groups.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Crop Sciencementioning

confidence: 99%

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Genetic variation of the fungus Atkinsonella hypoxylon infecting poverty oat grass

2020

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“…Following the methods described by Thammina et al. (2017), the program MISA identified 6,554 simple sequence repeats in ‘BCD’, 10,179 in ‘Declaration’, and 10,451 in ‘Providence’. Reciprocal BLAST hits were used to identify the most similar sequences across the three libraries and those reciprocal hits were compared with the MISA output to identify 1,478 reciprocal hits containing SSRs.…”

Section: Resultsmentioning

confidence: 99%

Colonial bentgrass transcript‐expression differences compared with creeping bentgrass in response to water‐deficit stress

Amundsen

Warnke²,

Bushman

et al. 2021

Crop Science

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Natural stands of creeping bentgrass (Agrostis stolonifera) are often found in wetland areas and exhibit very poor tolerance to dry soils. Colonial bentgrass (A. capillaris) is frequently found in drier habitats and has the ability to go dormant and recover quickly under water‐deficit stress. Hybridization between creeping and colonial bentgrass is possible and a better understanding of gene regulation under water‐deficit conditions of the two species could improve breeding strategies for water deficit–tolerant bentgrasses. A greenhouse dry‐down study was conducted using two creeping bentgrass clones with differing water use profiles and one colonial bentgrass clone. The genotypes were exposed to water‐deficit stress and well‐watered control genotypes were included. Gravimetric evapotranspiration was determined, and control plants watered at 80% ET and deficit irrigation plants watered at 50% ET. Water use rates were similar among all plants with the creeping bentgrass genotypes exhibiting stress earlier and recovering more slowly than the colonial bentgrass. At the conclusion of the experiment, ribonucleic acid sequencing analysis was conducted and there were 975 differentially‐expressed colonial bentgrass transcripts in response to water‐deficit stress compared with an average 98 differentially‐expressed creeping bentgrass transcripts. Among the colonial bentgrass upregulated transcripts in response to water‐deficit stress were eight transcription factors previously shown to be involved in deficit water stress response and several transposon‐related transcripts. This study characterized several transcripts with unique expression changes in colonial bentgrass compared with creeping bentgrass, which may explain why colonial bentgrass is more drought tolerant than creeping bentgrass.

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“…The formation of the fungal “choke” generally occurs at the lowest node of the tiller that produces a nodal seed head (Figure 2). In the literature, the two seed heads are often referred to as chasmogamous (terminal) and cleistogamous (nodal) seed heads (Clay, 1982); however, this terminology is misleading, as D. spicata terminal flowers very rarely open and do not typically form viable anthers or pollen, making both seed heads functionally cleistogamous (Phillipson, 1986; Thammina, Amundsen, Bushman, Kramer, & Warnke, 2018; Weatherwax, 1928).…”

Section: Introductionmentioning

confidence: 99%

PCR‐based detection of the epibiotic fungus Atkinsonella hypoxylon associated with its host grass Danthonia spicata

Warnke

2020

Crop Science

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Danthonia spicata (L.) Beauv., commonly known as poverty oatgrass, is a perennial bunch-type grass native to North America. Danthonia spicata is often managed as a turfgrass in areas of the United States where cool-season grasses are adapted, and it has potential for development as a low-input turfgrass option. Naturally occurring D. spicata plants occasionally exhibit "choke" of the flowering stems due to a proliferation of fungal hyphae by the ascomycete Atkinsonella hypoxylon (Peck) Diehl. (family Clavicipitaceae). Polymerase chain reaction (PCR) primers were designed to amplify a 116-bp A. hypoxylon-specific fragment within the 5.8S ribosomal RNA Internal Transcribed Spacer 1 (ITS 1) region. The primer set was then used to amplify the fragment from 24 A. hypoxylon isolates and 24 D. spicata terminal seed head DNA extractions collected from a greenhouse-maintained population that has never exhibited choke. The A. hypoxylon ITS 1 fragment was amplified from all fungal samples and all terminal seed head samples tested. The plant and fungal amplified fragments produced the same melt peak in high-resolution melt analysis, and sequence analysis of all terminal seed head samples showed highly significant blast hits with the ITS 1 sequence of A. hypoxylon GenBank Accession U57405.1 and the 24 A. hypoxylon isolates. These results provide evidence that all sampled D. spicata plants are associated with the fungus A. hypoxylon and provide a useful tool for the study A. hypoxylon and its association with D. spicata. 1660wileyonlinelibrary.com/journal/csc2 Crop Science. 2020;60:1660-1665.How to cite this article: Warnke SE. PCR-based detection of the epibiotic fungus Atkinsonella hypoxylon associated with its host grass Danthonia spicata.

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Genetic diversity of Danthonia spicata (L.) Beauv. based on genomic simple sequence repeat markers

Cited by 5 publications

References 30 publications

Genetic variation of the fungus Atkinsonella hypoxylon infecting poverty oat grass

Genetic variation of the fungus Atkinsonella hypoxylon infecting poverty oat grass

Colonial bentgrass transcript‐expression differences compared with creeping bentgrass in response to water‐deficit stress

PCR‐based detection of the epibiotic fungus Atkinsonella hypoxylon associated with its host grass Danthonia spicata

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