Genetic Diversity of Cultivable Plant Growth-Promoting Rhizobacteria in Korea

Kim, Won Il; Cho, Won Kyong; Kim, Su Nam; Chu, Hyo Sub; Ryu, Ki‐Hyun; Yun, Jong Chul; Park, Chang Seuk

doi:10.4014/jmb.1101.01031

Cited by 34 publications

(25 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We selected the strain B1-9, which was previously identified to promote growth of cucumber plants (Kim et al, 2011). To test the ability of B1-9 to increase plant growth, we Fig.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Kim¹,

Cho²,

Kim³

et al. 2012

The Plant Pathology Journal

Self Cite

View full text Add to dashboard Cite

The bacteria B1-9 that was isolated from the rhizosphere of the green onion could promote growth of pepper, cucumber, tomato, and melon plants. In particular, pepper yield after B1-9 treatment on the seedling was increased about 3 times higher than that of control plants in a field experiment. Partial 16S rDNA sequences revealed that B1-9 belongs to the genus Pantoea ananatis. Pathogenecity tests showed non-pathogenic on kimchi cabbage, carrot, and onion. The functional characterization study demonstrated B1-9's ability to function in phosphate solubilization, sulfur oxidation, nitrogen fixation, and indole-3-acetic acid production. To trace colonization patterns of B1-9 in pepper plant tissues, we used DRAQ5™ fluorescent dye, which stains the DNAs of bacteria and plant cells. A large number of B1-9 cells were found on the surfaces of roots and stems as well as in guard cells. Furthermore, several colonized B1-9 cells resided in inner cortical plant cells. Treatment of rhizosphere regions with strain B1-9 can result in efficient colonization of plants and promote plant growth from the seedling to mature plant stage. In summary, strain B1-9 can be successfully applied in the pepper plantation because of its high colonization capacity in plant tissues, as well as properties that promote efficient plant growth.

show abstract

“…We selected the strain B1-9, which was previously identified to promote growth of cucumber plants (Kim et al, 2011). To test the ability of B1-9 to increase plant growth, we Fig.…”

Section: Resultsmentioning

confidence: 99%

“…showing high growth promotion were collected from various regions (Kim et al, 2011). Root and rhizosphere samples were ground with pestle and mortar and diluted with 0.1 M MgSO 4 .…”

Section: Isolation Of Rhizobacteria From Plants Various Plantsmentioning

confidence: 99%

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Kim¹,

Cho²,

Kim³

et al. 2012

The Plant Pathology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…En el suelo, la microbiota interviene en procesos dinamizadores de nutrientes como la fijación del nitrógeno, la solubilización de fosfatos y la degradación de compuestos carbonados, influyendo en el contenido de materia orgánica, la estabilidad y fertilidad del suelo, contribuyendo al establecimiento y desarrollo de las comunidades vegetales (Kim et al, 2011;Waring et al, 2014). Con el fin de conocer la abundancia de microorganismos cultivables y determinar grupos funcionales en el suelo rizosférico paramuno, en este trabajo se determinó la concentración celular bacteriana y fúngica cultivable asociada a la rizósfera de Espeletia spp.…”

Section: Introductionunclassified

“…Estas bacterias se han reportado también en suelos con cultivos agrícolas y se caracterizan por descomponer la materia orgánica, hacer disponible el fósforo y por producir compuestos que promueven el crecimiento de las plantas. Sin embargo, algunas especies se han reportado como fitopatógenas (Kim et al, 2011).…”

unclassified

Rizospheric Microbiota of Espeletia spp. from Santa Inés and Frontino-Urrao Paramos in Antioquia, Colombia

Medina¹,

Vásquez²

2014

Acta biol. colomb.

View full text Add to dashboard Cite

“…A nonpathogenic P. ananatis strain, B1-9, was isolated from rhizosphere of green onion in Korea and was capable of promoting plant growth. This strain has phosphate solubilization activity, engages in nitrogen fixation and siderophore production, and produces high levels of indole (5). Root drenching with P. ananatis B1-9 enhanced red pepper crop yield in a field about 3 times (4).…”

mentioning

confidence: 99%

Draft Genome Sequence of Pantoea ananatis B1-9, a Nonpathogenic Plant Growth-Promoting Bacterium

et al. 2012

View full text Add to dashboard Cite

Pantoea ananatis B1-9 is an endophytic Gram-negative rhizobacterium that was isolated for its ability to promote plant growth and improve crop yield in the field. Here we report the draft genome sequence of P. ananatis B1-9. Comparison of this sequence to the sequenced genome of a plant-pathogenic P. ananatis strain, LMG20103, indicated that the pathogenesis-related genes were absent, but a subset of gene functions that may be related to its plant growth promotion were present. Pantoea ananatis is one of a number of well-known bacterial species that cause diverse symptoms, depending on their hosts, and are biocontrol agents possessing antifungal and antibacterial properties (2). A nonpathogenic P. ananatis strain, B1-9, was isolated from rhizosphere of green onion in Korea and was capable of promoting plant growth. This strain has phosphate solubilization activity, engages in nitrogen fixation and siderophore production, and produces high levels of indole (5). Root drenching with P. ananatis B1-9 enhanced red pepper crop yield in a field about 3 times (4). Previously, the genome sequence of a pathogenic P. ananatis strain was determined (3), but the genome sequence of a biocontrol agent of P. ananatis has not been available.The genomic DNA of P. ananatis B1-9 was isolated, and a library was prepared from a sheared-DNA fraction of ϳ300 bp using Illumina paired-end sample preparation kits according to the manufacturer's instructions. This library was sequenced on an Illumina genome analyzer II (Illumina, San Diego, CA) for 76 cycles, generating over 6 million good-quality paired-end reads amounting to over 400 million nucleotides (nt). The short-read sequences were assembled using Velvet version 0.7.55 (7, 8) into a sequence with an empirically determined optimal hash length of 39 nt and a minimum contig length of 150 nt. The assemblies were uploaded to the automated annotation platform RAST (Rapid Annotation using Subsystems Technology) server maintained by the National Microbial Pathogen Data Resource (1) and visualized with the SEED viewer (6).This shotgun genome sequence of P. ananatis B1-9 has a total of 5,105,557 nt. The annotation indicates that 169 contigs harbor a total of 4,988 protein-encoding genes (PEGs). Sequence coverage was 62-fold or greater for 99% of the annotated genes. Additionally, 81.7% of the annotated PEGs were greater than 300 nt in length. The assembly did not adequately reconstruct the rRNA genes, but 65 tRNA sequences were identified. Preliminary annotation of the draft genome sequence of P. ananatis B1-9 did not show any homologues to genes involved in pathogenesis, but several type IV secretion system homologues were identified. Potential homologues to genes involved in plant growth promotion and yield improvement were identified in the B1-9 genome sequence. The genome sequences of P. ananatis B1-9 will provide useful information and insights into plant growth promotion and improvement in crop yield.Nucleotide sequence accession numbers. The assembled shotgun genome sequence and anno...

show abstract

Genetic Diversity of Cultivable Plant Growth-Promoting Rhizobacteria in Korea

Cited by 34 publications

References 54 publications

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

Rizospheric Microbiota of Espeletia spp. from Santa Inés and Frontino-Urrao Paramos in Antioquia, Colombia

Draft Genome Sequence of Pantoea ananatis B1-9, a Nonpathogenic Plant Growth-Promoting Bacterium

Contact Info

Product

Resources

About