Genetic diversity of cucumber genotypes revealed by SSR markers and agronomic traits

Abstract

Understanding the level of genetic diversity and structure of landraces is essential for economical use of genetic resources. In the present study, we investigated the genetic diversity of 36 representative sample of cucumber genotypes based on 10 quantitative traits and 17 polymorphic simple sequence repeat (SSR) markers. Our result revealed variability in flowering behavior, growth habit and fruit characters. We found that the traits, days to opening of first female flower varied from 39.03 to 51.94, fruit length 6.09 to 30.01(cm), fruit weight 104.39 to 277.05(g) and yield per plant 699.38 to 1670.93(g). Genetic distance based on 17 SSR markers among 36 genotypes was quantified ranging from 0.03 to 0.70 indicated a wide diversity among the genotypes selected for evaluation in the present study. These primers produced 2-4 number of alleles with an average 2.76. Polymorphism information content varied from 0.005 to 0.550 with an average of 0.348. The observed heterozygosity mean was 0.098, while gene diversity or expected heterozygosity was 0.625 to 0.057. The distinct genotypes found in this study based on 406

Phylogeny and diversity of lamiaceae based on rps14 gene in Pakistan

“…Thus, researchers have examined the idea that an easy and rapid way of species identification and defining species boundaries is possible with the use of short DNA sequence (ERIĆ et al, 2019;SEVINDIK, 2019), which possess a standardized position in the genome, called as DNA barcode (HEBERT et al, 2004;FRÉZAL and LEBLOIS, 2008). DNA barcoding is a new approach designed to provide rapid, accurate and automatable species identification by using short standardized gene regions as internal species tags (AKINRO et al, 2019;GRITSENKO et al, 2019;PATI et al, 2019). Molecular identification methods have been used in forensics, food and taxonomy-based identification (TELETCHEA et al, 2008).…”