Genetic Diversity of Cowpea Mild Mottle Irus on Soybean in Several Region in Indonesia

Sutrawati, Mimi; Hidayat, Sri Hendrastuti; Sukarno, Bonny Purnomo Wahyu; Suastika, Gede; Nurmansyah, Ali

doi:10.5220/0009935718151819

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“…Virus dapat menyebar ke bagian tanaman yang masih muda dengan cepat ini disebabkan tanaman muda belum mempunyai sistem pertahanan yang kuat (Sudiono et al 2001). Sutrawati et al, (2020) melaporkan adanya infeksi Begomovirus pada tanaman papaya yang berada di provinsi Bengkulu dengan insidensi penyakit hingga 100 %. Penyakit virus terbawa benih oleh Begomovirus menjadi penting karena mampu menjadi sumber inokulum awal di lahan pertanaman.…”

Section: Pendahuluanunclassified

“…Berdasarkan hasil pengamatan di lapangan pada lokasi pertama di desa Pematang Donok, kecamatan Kabawetan pepaya gejala mosaik kuning tersebut sejak berumur kurang lebih 3 bulan dengan penyebaran yang cukup cepat disebabkan oleh kondisi lahan yang ditumbuhi gulma yang menjadi tempat tinggal untuk beberapa serangga, gejala mosaik kuning ini dapat menyebabkan pohon papaya menjadi kerdil (tumbuh secara tidak normal) serta daun yang menggulung ke atas dan beberapa ukuran buah yang tidak berkembang secara normal. Hal ini sesuai dengan penelitian yang dilakukan oleh Sutrawati et al, (2020) bahwa beberapa kebun papaya di temukannya gejala sistemik dari mosaik kuning, daun menggulung ke atas, kerdil pada tangkai daun dan binti-bintik hijau pada buah papaya.…”

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Deteksi Begomovirus Pada Benih Pepaya Dan Pengendaliannya Dengan Metode Hot Water Treatment

Pangesti

Sutrawati²,

Bustamam³

2022

Agropross

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Begomovirus is a virus that has been reported as the cause of yellow leaf curl disease on papaya plants in Bengkulu. Incidence of yellow mosaic disease on papaya plants in Bengkulu up to 100% in papaya cultivation centers. So that it strengthens the suspicion of the potential for seed transmission Begomovirus in papaya plants. Seed-borne viruses by Begomovirus are important because : can be a source of initial inoculum in the planted area. Then the control of the virus is carried by the seeds. It is important to suppress the initial source of inoculum in the field. This research aims to Begomovirus detection in papaya seeds was carried out by PCR method using SPG1 . Primer and SPG 2, and control of virus in seeds by controlling Hot Water Treatment. Seed Papaya plants infected with Begomovirus were given hot water treatment which consisted of two treatments temperature is 56℃ for 60 minutes and 58℃ for 40 minutes in a water bath. positive control are seeds that were confirmed positive for begomovirus without Hot Water Treatment (HWT) while the negative control seeds were confirmed negative for begomovirus. The results of this study showed that Begomovirus was not detected at the age of 7 (primary leaves) days after sowing, however detected in plants aged 14 days after sowing (first leaf) using primers universal SPG 1 and SPG 2. After the papaya seeds were treated (HWT with a temperature of 56℃ for 60 minutes and 58℃ for 40 minutes showed negative results in plants aged 7 days after seedlings and 14 days after sowing. This shows that the HWT treatment is able to suppres Begomovirus in papaya seeds.

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