Genetic diversity of common bean accessions from former Yugoslav Republic of Macedonia as revealed by molecular and morphological markers

Abstract

Cultivation of common bean has a long tradition in the Former Yugoslav Republic of Macedonia (FYROM) and is still nowadays important part of the human diet. In a study reported here 71 accessions from the FYROM were assessed for genetic diversity with the aim to provide information on genetic structure of Macedonian common bean germplasm and to depict its peculiarities. A total of 71 accessions were assessed using 13 microsatellite and 16 morphological markers. The average number of alleles per microsatellite was 5.8, and ranged from three to 16 alleles. High capacity of selected markers for distinguishing genotypes was identified by the calculation of a very low value of probability of identity. The relationship among 71 studied accessions was assessed by hierarchical cluster analysis. A very clear separation of accessions into two groups was observed in the UPGMA dendrogram. The larger represented Andean gene pool and contained 40 accessions (56% of total), while the other 31 accessions (44% of total) composed Mesoamerican gene pool. The two groups were successfully discriminated by eight morphological traits. Within the larger Andean cluster in the UPGMA dendrogram a sub-group of 16 climbing accessions was separated from 24 bush accessions. The absence of the string in the pods of the climbers suggests that this sub-group comprises snap beans grown primarily for their fresh pods. There were eight morphological traits in total that distinguished the two Andean sub-groups. Assessment of genetic relationship among accessions, their classification into respective gene pool and identification of morphological peculiarities provided valuable information for the management of plant gene bank and Macedonian bean breeding program.

Morphological traits associated with anthracnose (Colletotrichum lindemuthianum) resistance in selected common bean (Phaseolus vulgaris L.) genotypes

“…The length of the common bean stem fifth internode on the main stem measured in the fifteen genotypes showed some discrimination between the resistant, tolerant and susceptible genotypes. The measurements varied among all the genotypes and these results are in agreement with the studies made by Maras et al (2016) who reported the stem length of fifth internode on the main stem of common bean genotypes to be associated with disease incidence and severity cases. Environment x genotype interaction was not significant but the genotypes were highly significant at p ≤ 0.001.…”

“…Visual observation made on the fifteen genotypes revealed broadly ovate leaflets with acuminate apices, the petial of the terminal leaflets were longer than those below. Past research made by Buah et al (2017) and Maras et al (2016) classified bracteole shape of plant leaves into; cordate, ovate, lanceolate and triangle. Therefore the recent research characterized the fifteen bean genotypes under field study as having one variable (100%), which was ovate leave shape and therefore there was no significance of leaf shapes in anthracnose resistance.…”

Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe

“…Although the variability of common-bean accessions from central and South-Eastern Europe has already been reported in terms of morphological and molecular markers [35][36][37][38], such information on the runner bean from this region was lacking. Recently, runner-bean seeds of a Slovenian collection were characterised morphologically [14], although there remain few data for other traits, and none for runner beans from the other South-Eastern European countries covered here.…”

“…Morpho-agronomic information in this study will help to promote runner-bean breeding for the development of new varieties with favourable traits. To date, only limited information is available on runner-bean accessions from South-Eastern Europe [14,37,39], as compared to the common bean [14,[35][36][37][38]40]. As a further step, analysis of this diversity using molecular approaches is recommended, to provide more information on the overall genetic diversity, and potentially on the genes responsible for the specific morpho-agronomic traits in this runner-bean collection from South-Eastern Europe.…”

“…Additionally, Group 1 included the commercial varieties 'Bonela' from the trials in Slovenia, Serbia, and Bosnia and Herzegovina, and 'Darko' from the trials in Slovenia, Serbia and North Macedonia. Group 2 included most of the accessions that originated from Slovenia (38), all of those from Bosnia and Herzegovina (10), five accessions from Serbia, and one from North Macedonia. Additionally, Group 2 included the commercial varieties 'Emergo' from all of the five country trials, 'Bonela' from the trials in Romania and North Macedonia, and 'Darko' from the trials in Romania.…”