Genetic diversity of Chinese natural bermudagrass (Cynodon dactylon) germplasm using ISSR markers

Li, Huiying; Liu, Li; Lou, Yanhong; Hu, Tao; Fu, Jinmin

doi:10.1016/j.scienta.2010.12.001

Cited by 22 publications

(18 citation statements)

References 23 publications

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“…Both marker types have been successfully used for genetic analyses of warm-season turfgrasses, such as Zoysia japonica Steud. (Chen et al, 2009;Xie et al, 2012), Cynodon dactylon (Huang et al, 2010;Li et al, 2011;Ling et al, 2010;Wang et al, 2009bWang et al, , 2013Yi et al, 2008), Cynodon arcuatus J. Presl (Huang et al, 2013), and Eremochloa ophiuroides (Munro) Hack. (Zheng et al, 2009(Zheng et al, , 2013.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers

Zhang¹,

Liao²,

Wang³

et al. 2016

horts

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Molecular genetic diversity and relationships among 86 Chrysopogon aciculatus (Retz.) Trin. accessions were assessed using intersimple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. Twenty-five ISSR markers generated 283 amplification bands, of which 266 were polymorphic. In addition, 576 polymorphic bands were detected from 627 bands amplified using 30 SRAP primers. Both marker types revealed a high level of genetic diversity, with ISSR markers showing a higher proportion of polymorphic loci (PPL; 94%) than SRAP markers (91.87%). The ISSR and SRAP data were significantly correlated (r = 0.8023). Cluster analysis of the separate ISSR and SRAP data sets clustered the accessions into three groups, which generally were consistent with geographic provenance. Cluster analysis of the combined ISSR and SRAP data set revealed four major groups similar to those based solely on ISSR or SRAP markers. The findings demonstrate that ISSR and SRAP markers are reliable and effective tools for analysis of genetic diversity in C. aciculatus.

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Section: Discussionmentioning

confidence: 99%

Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers

Zhang¹,

Liao²,

Wang³

et al. 2016

horts

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“…in Chinese natural bermudagrass ( Cynodon dactylon ) germplasm using ISSR markers [22]. This showed that there was an incomplete direct relationship between the origin of the accessions and the molecular clusters.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers

Farsani

Etemadi

Sayed-Tabatabaei

et al. 2011

IJMS

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Bermudagrass (Cynodon spp.) is a major turfgrass for home lawns, public parks, golf courses and sport fields and is known to have originated in the Middle East. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences between them are often subtle and subjected to environmental influences. In this study, twenty seven bermudagrass accessions and introductions, mostly from different parts of Iran, were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. Fourteen ISSR primers amplified 389 fragments of which 313 (80.5%) were polymorphic. The average polymorphism information content (PIC) was 0.328, which shows that the majority of primers are informative. Cluster analysis using the un-weighted paired group method with arithmetic average (UPGMA) method and Jaccard’s similarity coefficient (r = 0.828) grouped the accessions into six main clusters according to some degree to geographical origin, their chromosome number and some morphological characteristics. It can be concluded that there exists a wide genetic base of bermudograss in Iran and that ISSR markers are effective in determining genetic diversity and relationships among them.

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“…ISSR-PCR was carried out according to Li et al [63] as follows: the total reaction volume was 15 μL and contained 20 ng template DNA, approximately 1.0 μM primer, 7.5 μL Mix (10 × PCR buffer, Mg 2+ , dNTPs; Tiangen Biotech, Beijing, China), and 1 U Taq polymerase. Amplifications were performed in a BioRad iCycle PCR machine (BIO-RAD Certified) under the following conditions: 95 °C for 5 min, followed by 35 cycles of the following: 95 °C for 45 s, 52–55 °C for 45 s, and 72 °C for 90 s. A final extension was conducted at 72 °C for 7 min.…”

Section: Methodsmentioning

confidence: 99%

Genetic variation, population structure and linkage disequilibrium in Switchgrass with ISSR, SCoT and EST-SSR markers

et al. 2016

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BackgroundTo evaluate genetic variation, population structure, and the extent of linkage disequilibrium (LD), 134 switchgrass (Panicum virgatum L.) samples were analyzed with 51 markers, including 16 ISSRs, 20 SCoTs, and 15 EST-SSRs.ResultsIn this study, a high level of genetic variation was observed in the switchgrass samples and they had an average Nei’s gene diversity index (H) of 0.311. A total of 793 bands were obtained, of which 708 (89.28 %) were polymorphic. Using a parameter marker index (MI), the efficiency of the three types of markers (ISSR, SCoT, and EST-SSR) in the study were compared and we found that SCoT had a higher marker efficiency than the other two markers. The 134 switchgrass samples could be divided into two sub-populations based on STRUCTURE, UPGMA clustering, and principal coordinate analyses (PCA), and upland and lowland ecotypes could be separated by UPGMA clustering and PCA analyses. Linkage disequilibrium analysis revealed an average r2 of 0.035 across all 51 markers, indicating a trend of higher LD in sub-population 2 than that in sub-population 1 (P < 0.01).ConclusionsThe population structure revealed in this study will guide the design of future association studies using these switchgrass samples.

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Genetic diversity of Chinese natural bermudagrass (Cynodon dactylon) germplasm using ISSR markers

Cited by 22 publications

References 23 publications

Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers

Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers

Assessment of Genetic Diversity of Bermudagrass (Cynodon dactylon) Using ISSR Markers

Genetic variation, population structure and linkage disequilibrium in Switchgrass with ISSR, SCoT and EST-SSR markers

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