Genetic diversity of Ceratoides arborescens, a species endemic to China, detected by inter-simple sequence repeat (ISSR)

Abstract

ABSTRACT. In order to investigate genetic diversity and population structure of Ceratoides arborescens, six populations were selected from different steppe types in Inner Mongolia grasslands of China. Intersimple sequence repeat (ISSR) markers were used to assess the genetic diversity within and among natural populations of C. arborescens. Thirteen ISSR primers generated 154 discernible DNA bands, of which 151 (98.05%) were polymorphic. High genetic diversity was detected at the species level [percentage of polymorphic loci (PPB) = 98.05%; H = 0.2984; I = 0.4557], whereas, relatively low genetic diversity existed within populations (PPB = 80.62%; H = 0.2675; I = 0.4031). Analysis of molecular variance showed that variation existed mainly within populations (73.25%) rather than among populations (26.75%), which was in line with the high level of gene flow (N m = 4.3332). The Mantel test found no significant correlation between genetic distance and geographic distance (r = 0.7522, P < 0.05). Six populations were clustered into two main groups, a desert steppe group and a typical steppe group.

Molecular Characterization of Tinospora cordifolia (Willd.) Miers Using Novel g-SSR Markers and Their Comparison with EST-SSR and SCoT Markers for Genetic Diversity Study

“…The Polymorphic Information Content ( PIC ) in Tinospora varied from 0.03 to 0.53 with a mean value of 0.33, which is less than the mean PIC observed in other medicinal plants. Cheng et al [ 42 ] observed a PIC value greater than 0.7 in Paeonia lactiflora , a PIC value of 0.76 was observed in Astragalus mongholicus [ 43 ], and a value of more than 0.7 was seen in Smilax brasiliensis [ 44 ]. However, there are reports that showed low PIC values such as in the case of Groundnut (0.25) [ 39 ] and Moringa (0.15) [ 45 ].…”

“…In the common bean Phaseolus vulgaris , the PCoA also showed a separation of 53% along coordinate 1 and 13% along coordinate 2 [ 46 ]. In the grass pea, the first two principal components explained 43.42% and 29.17% of the molecular variance, respectively [ 43 ]. Population Structure analysis also showed differentiation among T. cordifolia , T. sinensis , and T. rumphii study based on g-SSR markers.…”

Nuclear genome size analysis in Krascheninnikovia ceratoides s. l. (Amaranthaceae)

“…At the same time, PérezCollazos & Catalán (2007) showed a sufficiently high genetic variability of two tetraploid populations of K. ce ra to ides from Spain by studying more variable DNA sec tions by ISSR analysis. This analysis was also used by Wang et al (2015) for K. arborescens in China. It was found that K. ar bo res cens possesses an unexpectedly high rate of genetic di ver sity in the studied area.…”