Genetic Diversity of Bradyrhizobial Populations from Diverse Geographic Origins that Nodulate Lupinus spp. and Ornithopus spp.

Jarabo-Lorenzo, A.; Pérez-Galdona, Ricardo; Donate-Correa, Javier; Rivas, Raúl; Velázquez, Encarna; Hernández, Mariano; Temprano, F.; Martínez‐Molina, Eustoquio; Ruiz-Argüeso, Tomás; León-Barrios, Milagros

doi:10.1078/072320203770865927

Cited by 72 publications

(49 citation statements)

References 40 publications

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“…This work has differentiated three major clades among the Bradyrhizobium nodA sequences, one of which, called clade II, was composed exclusively of the strains isolated from lupins, broom (Cytisus scoparius), and serradella. Similar phylogenetic patterns detected for nodC and nifH gene sequences corroborated the unique position of the symbiotic genes of lupin and serradella bradyrhizobia (15,49,50). These findings led to the delineation of bradyrhizobia infecting legumes of the Genisteae and Loteae tribes in a new biovariety, genistearum.…”

supporting

confidence: 52%

European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa

Stępkowski

Moulin

Krzyżańska

et al. 2005

Appl Environ Microbiol

164

119

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We applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin Bradyrhizobium strains that persist in soils of Western Australia and South Africa. The selected strains belonged to different randomly amplified polymorphic DNA (RAPD)-PCR clusters that were distinct from RAPD clusters of applied inoculant strains. Phylogenetic analyses were performed with nodulation genes (nodA, nodZ, nolL, noeI), housekeeping genes (dnaK, recA, glnII, atpD), and 16S-23S rRNA intergenic transcribed spacer sequences. Housekeeping gene phylogenies revealed that all serradella and Lupinus cosentinii isolates from Western Australia and three of five South African narrow-leaf lupin strains were intermingled with the strains of Bradyrhizobium canariense, forming a well supported branch on each of the trees. All nodA gene sequences of the lupin and serradella bradyrhizobia formed a single branch, referred to as clade II, together with the sequences of other lupin and serradella strains. Similar patterns were detected in nodZ and nolL trees. In contrast, nodA sequences of the strains isolated from native Australian legumes formed either a new branch called clade IV or belonged to clade I or III, whereas their nonsymbiotic genes grouped outside the B. canariense branch. These data suggest that the lupin and serradella strains, including the strains from uncultivated L. cosentinii plants, are descendants of strains that most likely were brought from Europe accidentally with lupin and serradella seeds. The observed dominance of B. canariense strains may be related to this species' adaptation to acid soils common in Western Australia and South Africa and, presumably, to their intrinsic ability to compete for nodulation of lupins and serradella.

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supporting

confidence: 52%

European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa

Stępkowski

Moulin

Krzyżańska

et al. 2005

Appl Environ Microbiol

164

119

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“…Plants of the genus Lupinus, which accomodates around 250 species of herbs and shrubs distributed in the Mediterranean area and in the American continent, are nodulated by Bradyrhizobium spp. (Jarabo-Lorenzo et al, 2003;Vinuesa et al, 2005;Stepkowski et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius

et al. 2010

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Our current knowledge of plant-microbe interactions indicate that populations inhabiting a host plant are not restricted to a single microbial species but comprise several genera and species. No one knows if communities inside plants interact, and it has been speculated that beneficial effects are the result of their combined activities. During an ecological study of nitrogen-fixing bacterial communities from Lupinus angustifolius collected in Spain, significant numbers of orangepigmented actinomycete colonies were isolated from surface-sterilized root nodules. The isolates were analysed by BOX-PCR fingerprinting revealing an unexpectedly high genetic variation. Selected strains were chosen for 16S rRNA gene sequencing and phylogenetic analyses confirmed that all strains isolated belonged to the genus Micromonospora and that some of them may represent new species. To determine the possibility that the isolates fixed atmospheric nitrogen, chosen strains were grown in nitrogen-free media, obtaining in some cases, significant growth when compared with the controls. These strains were further screened for the presence of the nifH gene encoding dinitrogenase reductase, a key enzyme in nitrogen fixation. The partial nifH-like gene sequences obtained showed a 99% similarity with the sequence of the nifH gene from Frankia alni ACN14a, an actinobacterium that induces nodulation and fixes nitrogen in symbiosis with Alnus. In addition, in situ hybridization was performed to determine if these microorganisms inhabit the inside of the nodules. This study strongly suggests that Micromonospora populations are natural inhabitants of nitrogen-fixing root nodules.

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“…The nifH and nodC phylogenies correlated well with the host range of the ECGL isolates (Jarabo-Lorenzo et al, 2003;Vinuesa et al, 2005), but were incongruent with the maximum-likelihood species phylogeny (Felsenstein, 2004;Nichols, 2001) inferred from combined and congruent glnII plus recA (compare Figs C and D, available as supplementary material in IJSEM Online) partitions (Vinuesa et al, 2005). Regardless of their geographical origin and (geno)species assignation, all isolates from genistoid legumes and Ornithopus spp.…”

mentioning

confidence: 94%

“…At the molecular level this species can be easily distinguished from strains of its sister species B. japonicum and all other described Bradyrhizobium species by the unique 16S rRNA PCR-RFLP genotype obtained with the endonucleases HhaI, DdeI and HinfI (Jarabo- Lorenzo et al, 2000Lorenzo et al, , 2003Vinuesa et al, 1998Vinuesa et al, , 1999Vinuesa et al, , 2005. B. canariense strains also display a distinct fingerprint of stable low-molecular-weight RNAs (Jarabo-Lorenzo et al, 2000).…”

mentioning

confidence: 99%

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Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta

Vinuesa

León-Barrios

Silva

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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248

136

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Genetic Diversity of Bradyrhizobial Populations from Diverse Geographic Origins that Nodulate Lupinus spp. and Ornithopus spp.

Cited by 72 publications

References 40 publications

European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa

European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa

The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius

Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta

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